Knockdown of DPAP3 does not affect maturation of SUB1 substrates.

<p>Representative western blots (n = 3–5) of schizont (Sch) and merozoite (M) lysates and culture supernatant (CS) collected after egress (SERA5) or schizont lysates (RAP1, RAMA) made from infected RBCs grown in the presence (+) or absence (-) of 2.5 mM GlcN. Densitometry of bands relative to the EXP2 loading control and between treatment groups revealed no significant difference in the processing of SERA5, RAP1 and RAMA as a result of DPAP3 knockdown.</p

The localization of DPAP3 is distinct from proteins that localize to the apical organelles.

<p><b>A.</b> IFA on RBCs infected with PfDPAP3-HAglmS parasites and fixed with acetone/methanol. DPAP3 is labeled with the anti-HA antibody. The scale bars represent 5 μm. <b>B.</b> Immuno-electron microscopy of PfDPAP3-HAglmS parasites with anti-HA antibody shows labelling for DPAP3 (as indicated by the arrowheads) in a mature schizont at the periphery of the rhoptry bulb (RB), rhoptry neck (RN) as well as at the PV/parasitophorous vacuole membrane (PVM).</p

DPAP3 expression in <i>P</i>. <i>falciparum</i> can be conditionally regulated.

<p><b>A.</b> Western blot analysis showing dose-dependent PfDPAP3-HA protein expression in two independent clones after treatment with glucosamine (GlcN) for one (upper panel) or two (lower panel) cell cycles (Cyc1 and Cyc2, respectively). EXP2 serves as the loading control. <b>B.</b> Densitometry of PfDPAP3 expression levels in PfDPAP3-HAglmS Clone 1 parasites grown in the presence or absence of glucosamine for one cycle revealed DPAP3 expression levels were knocked down by 91%. Error bars represent ± SEM from three independent experiments. <b>C.</b> IFA showing DPAP3-HA expression is significantly reduced within 24 hrs after addition of 2.5 mM GlcN when the parasites are at schizont stage. Scale bars represent 5 μm.</p