Appendix A. Hydrological seasonality in Taraira Lake, Lower Apaporis River, Colombia, during 2009.

Hydrological seasonality in Taraira Lake, Lower Apaporis River, Colombia, during 2009

Appendix D. Biweekly fruit and flower production in the flooded forest along the Apaporis River assessed during visual censuses.

Biweekly fruit and flower production in the flooded forest along the Apaporis River assessed during visual censuses

Appendix E. Percentage frequency of occurrence and average volumetric percentage contributed by 12 broad functional food categories to diets of six frugivorous fish species during three hydrological seasons.

Percentage frequency of occurrence and average volumetric percentage contributed by 12 broad functional food categories to diets of six frugivorous fish species during three hydrological seasons

Appendix F. Carbon and nitrogen isotope ratio biplots for six frugivorous fish species and food sources during three hydrological seasons.

Carbon and nitrogen isotope ratio biplots for six frugivorous fish species and food sources during three hydrological seasons

Appendix B. Methods of collection and preparation of samples for analysis of stable isotopes.

Methods of collection and preparation of samples for analysis of stable isotopes

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Supplementary material 2 from Body size–trophic position relationships among fishes of the lower Mekong basin

Body size is frequently claimed to be a major determinant of animal trophic interactions, yet few studies have explored relationships between body size and trophic interactions in rivers, especially within the tropics. We examined relationships between body size and trophic position (TP) within fish assemblages in four lowland rivers of the Lower Mekong Basin in Cambodia. Stable isotope analysis (based on δ¹⁵N) was used to estimate TP of common fish species in each river, and species were classified according to occupation of benthic versus pelagic habitats and major feeding guilds. Regression analysis yielded strong correlations between body size and TP among fishes from the Sesan and Sreprok rivers, but not those from the Mekong and Sekong rivers. The Mekong fish assemblage had higher average TP compared with those of other rivers. The relationship between body size and TP was positive and significantly correlated for piscivores and omnivores, but not for detritivores and insectivores. The body size–TP relationship did not differ between pelagic and benthic fishes. Body size significantly predicted TP within the orders Siluriformes and Perciformes, but not for Cypriniformes, the most species-rich and ecologically diverse order in the Lower Mekong River. We conclude that for species-rich, tropical fish assemblages with many detritivores and invertivores, body size would not be an appropriate surrogate for TP in food web models and other ecological applications

Appendix A. Estimations of source contributions from MixSIR model for Poecilla mexicana from the sulfidic cave, sulfidic surface stream, non-sulfidic cave, and non-sulfidic surface stream.

Estimations of source contributions from MixSIR model for Poecilla mexicana from the sulfidic cave, sulfidic surface stream, non-sulfidic cave, and non-sulfidic surface stream

Percent volume of diet categories in 23 species of Neotropical cichlids and their phylogenetic signal.

<p>Percent volume of diet categories in 23 species of Neotropical cichlids and their phylogenetic signal.</p

Percent volume for the three dominant diet categories of the 23 species studied.

<p>Categories are: benthic prey, epibenthic prey and fish. Species are arranged in decreasing order of percent benthic diet to illustrate the apparent pattern of exclusion between benthic and fish diets. Green stars show the distribution of the EBL, and orange species names indicate mouthbrooding lineages. See text and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0033997#pone-0033997-t001" target="_blank">Table 1</a> for more details.</p