21 research outputs found
Isolation of Bacterial Ribosomes with Monolith Chromatography
We report the development of a rapid chromatographic method for the isolation of bacterial ribosomes from crude cell lysates in less than ten minutes. Our separation is based on the use of strong anion exchange monolithic columns. Using a simple stepwise elution program we were able to purify ribosomes whose composition is comparable to those isolated by sucrose gradient ultracentrifugation, as confirmed by quantitative proteomic analysis (iTRAQ). The speed and simplicity of this approach could accelerate the study of many different aspects of ribosomal biology
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Transit of tRNA through the Escherichia coli ribosome: cross-linking of the 3 \u27 end of tRNA to ribosomal proteins at the P and E sites
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Peptidyl transferase and the movement of transfer RNA across the Escherichia coli ribosome.
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