Miokarditis vezan uz slinavku i šap u sisajuće teladi.

Foot-and-mouth disease (FMD) can lead to myocarditis in young animals, but the age distributions of calves with myocarditis have not been described, nor the biochemical profile in these calves. In an area endemic with foot-and-mouth disease, calves less than 6 months of age in infected farms were examined for clinical lesions and abnormalities in respiratory rate, heart rate and heart rhythm. In total, 53 calves were identified to be suspected of having foot-and-mouth disease infection. In 6 calves myocarditis was suspected based on tachypnea, tachycardia and gallop rhythm. In these 6 calves, cardiac troponin-I (cTnI) and aspartate aminotransferase (AST) were significantly higher (P<0.0001), but the levels of Creatinine Kinase MB (CK-MB) and Lactate dehydrogenase (LDH) were not. These 6 calves died within 2 days and histopathology confirmed myocarditis. All calves with myocarditis were younger than 2-months old, suggesting that myocarditis caused by FMD is mainly found in very young suckling calves.Slinavka i šap može dovesti do miokarditisa u mladih životinja. Dosada nije opisana dobna raspodjela miokarditisa ni biokemijski profil u teladi oboljele od slinavke i šapa. U jednom području gdje se slinavka i šap javlja endemijski, telad mlađa od šest mjeseci bila je na zaraženim farmama klinički pretražena posebice na poremećaje u frekvenciji bila, disanja i srčanog ritma. Ukupno su 53 teleta bila sumnjiva na slinavku i šap. Sumnja na miokarditis postavljena je u šest teladi i to na osnovi tahipneje, tahikardije i galopirajućeg ritma. U te su teladi razine srčanog troponina-I (cTnI) i aspartat-aminotransferaze (AST) bile značajno više (P<0,0001), dok razine kreatinin-kinaze MB (CK-MB) i laktat-dehidrogenaze (LDH) nisu. Tih šest teleta uginulo je unutar dva dana te je u njih miokarditis bio potvrđen patohistološki. Sva telad s miokarditisom bila je mlađa od dva mjeseca, što upućuje na zaključak da se miokarditis uzrokovan virusom slinavke i šapa pretežito javlja u sisajuće teladi najranije dobi

Procjena serumskoga srčanog troponina I u ovaca s akutnom mliječnokiselinskom acidozom buraga.

Cardiac troponin I (cTnI) is known to be a sensitive cardio biomarker to determine the myocardial damage in diseases affecting the cardiac muscles. However, there has not been sufficient research about cTnI concentration, which is the most sensitive indicator of myocardial damage in sheep with acute ruminal lactic acidosis (ARLA). For this reason this study aimed to evaluate the serum cTnI concentration in sheep with ARLA. Those diagnosed with ARLA (n = 20) from the total of 40 Akkaraman (White karaman) sheep, aged between 1-2 years used in this study comprised the affected group and the healthy ones (n = 20) comprised the control group. Ruminal fluid was obtained from the animals from both groups with the help of a stomach tube, and examined immediately. Blood samples were taken from the jugular vein of the sheep and the serum was separated. Serum cTnI concentration was measured with a commercial immunoassay system, using the one-step sandwich method. Serum enzyme (ALT, AST, CK-MB and LDH) activities were determined via a clinical biochemistry autoanalyzer. The average serum cTnI concentration was at the level of 0.035 ± 0.015 ng/mL (range; 0.02-0.06 ng/mL) in the control group sheep. It was determined that there was a substantial increase in the group with ARLA and the average concentration reached the level of 0.103 ± 0.080 ng/mL (range; 0.03-1.7 ng/mL) (P<0.0001). It was observed that another cardio marker, CK-MB, was found in the group with ARLA 454.50 ± 191.88 U/L (range; 214-861 U/L) and increased in comparison with the control group 224.35 ± 83.33 U/L (range; 133-421 U/L) (P<0.0001). An increase in LDH (P<0.001) and AST (P<0.001) from liver enzymes in the group with ARLA and ALT activities compared to the control group was identified (P<0.01). In conclusion, this present study determined that the serum cTnI concentration was high in sheep with ARLA and it was concluded that it could be useful to evaluate cTnI concentration as an important marker to determine the prognosis in sheep with ARLA.U bolestima koje zahvaćaju srčani mišić, srčani troponin I (cTnI) poznat je kao osjetljivi biomarker za određivanje oštećenja srčanog mišića. Ipak, nema puno istraživanja o koncentraciji cTnI kao najosjetljivijeg indikatora za oštećenje srčanog mišića kod ovaca s akutnom mliječno-kiselinskom acidozom buraga. Zbog navedenog, u ovom istraživanju namjera je bila procijeniti koncentraciju serumskog cTnI kod ovaca s akutnom mliječno-kiselinskom acidozom. Ovce s dijagosticiranom akutnom mliječno-kiselinskom acidozom (n = 20) u skupini od ukupno 40 akaraman (bijeli karaman) ovaca, u dobi od jedne do dvije godine, činile su pokusnu skupinu bolesnih jedinki. Preostale ovce (n = 20) činile su kontrolnu skupinu zdravih jedinki. Tekućina buraga od svih pretraženih životinja dobivena je sondiranjem i odmah analizirana. Uzorci krvi uzeti su iz jugularne vene te je iz njih izdvojen serum. Koncentracija serumskog cTnI mjerena je komercijalnim imunoenzimnim testom, sendvič postupkom. Aktivnosti serumskih enzima (ALT, AST, CK-MB i LDH) utvrđene su uporabom kliničkog biokemijskog autoanalizatora. Prosječna serumska koncentracija cTnI u kontrolnoj skupini ovaca bila je na razini 0,035 ± 0,015 ng/mL (raspon: 0,02 - 0,06 ng/mL). U skupini ovaca s acidozom utvrđeno je postojano povećanje koncentracije cTnI koja je dosegla razinu od 0,103 ± 0,080 ng/mL (raspon: 0,03 - 1,7 ng/mL) (P<0,0001). U pokusnoj skupini opaženo je povećanje i drugog srčanog markera CK-MB koji je u ovoj skupini iznosio 454,50 ± 191,88 U/I (raspon: 214 - 861 U/I), a u kontrolnoj 224,35 ± 83,33 U/I (raspon: 133 - 421 U/I) (P<0,0001). Također je ustanovljeno da su ovce s acidozom u odnosu na ovce kontrolne skupine imale povišene jetrene enzime LDH (P<0,001) i AST (P<0,001), odnosno ALT (P<0,01). Zaključeno je da ovce s acidozom imaju povećanu koncentraciju serumskog cTnI koji može poslužiti kao važan biljeg za prognozu bolesti

Evaluation of arginase activity, nitric oxide and oxidative stress status in sheep with contagious agalactia

It is known that inflammatory organ damages due to various agents, such as microorganisms including mycoplasmas, lead to oxidative stress. Nitric oxide (NO) functions as an antimicrobial agent, and arginase decreases proinflammatory cytokine release. There are very few studies on arginase activity, NO level and oxidative stress status in mycoplasmal infections. Therefore, the aim of this study was to evaluate erythrocyte arginase activity, plasma NO level and oxidative stress status in sheep with contagious agalactia. The study material consisted of 10 healthy sheep and 14 sheep with contagious agalactia characterised by mastitis, arthritis and keratoconjunctivitis. Erythrocyte arginase activity, plasma NO, malondialdehyde (MDA), total oxidant capacity (TOC) and total antioxidant capacity (TAC) levels were measured. Significant decreases in erythrocyte arginase activity and plasma TAC level (P < 0.001), and significant increases in plasma NO, MDA and TOC levels (P < 0.001) were found in the diseased sheep as compared with the healthy animals. This study suggests that contagious agalactia may cause oxidative stress due to increased plasma MDA and TOC levels and decreased plasma TAC levels, and that the decrease in erythrocyte arginase activity and increase in plasma NO level may contribute to the elimination of mycoplasmal agents causing contagious agalactia

Procjena serumskoga srčanog troponina I u ovaca s akutnom mliječnokiselinskom acidozom buraga.

Cardiac troponin I (cTnI) is known to be a sensitive cardio biomarker to determine the myocardial damage in diseases affecting the cardiac muscles. However, there has not been sufficient research about cTnI concentration, which is the most sensitive indicator of myocardial damage in sheep with acute ruminal lactic acidosis (ARLA). For this reason this study aimed to evaluate the serum cTnI concentration in sheep with ARLA. Those diagnosed with ARLA (n = 20) from the total of 40 Akkaraman (White karaman) sheep, aged between 1-2 years used in this study comprised the affected group and the healthy ones (n = 20) comprised the control group. Ruminal fluid was obtained from the animals from both groups with the help of a stomach tube, and examined immediately. Blood samples were taken from the jugular vein of the sheep and the serum was separated. Serum cTnI concentration was measured with a commercial immunoassay system, using the one-step sandwich method. Serum enzyme (ALT, AST, CK-MB and LDH) activities were determined via a clinical biochemistry autoanalyzer. The average serum cTnI concentration was at the level of 0.035 ± 0.015 ng/mL (range; 0.02-0.06 ng/mL) in the control group sheep. It was determined that there was a substantial increase in the group with ARLA and the average concentration reached the level of 0.103 ± 0.080 ng/mL (range; 0.03-1.7 ng/mL) (P<0.0001). It was observed that another cardio marker, CK-MB, was found in the group with ARLA 454.50 ± 191.88 U/L (range; 214-861 U/L) and increased in comparison with the control group 224.35 ± 83.33 U/L (range; 133-421 U/L) (P<0.0001). An increase in LDH (P<0.001) and AST (P<0.001) from liver enzymes in the group with ARLA and ALT activities compared to the control group was identified (P<0.01). In conclusion, this present study determined that the serum cTnI concentration was high in sheep with ARLA and it was concluded that it could be useful to evaluate cTnI concentration as an important marker to determine the prognosis in sheep with ARLA.U bolestima koje zahvaćaju srčani mišić, srčani troponin I (cTnI) poznat je kao osjetljivi biomarker za određivanje oštećenja srčanog mišića. Ipak, nema puno istraživanja o koncentraciji cTnI kao najosjetljivijeg indikatora za oštećenje srčanog mišića kod ovaca s akutnom mliječno-kiselinskom acidozom buraga. Zbog navedenog, u ovom istraživanju namjera je bila procijeniti koncentraciju serumskog cTnI kod ovaca s akutnom mliječno-kiselinskom acidozom. Ovce s dijagosticiranom akutnom mliječno-kiselinskom acidozom (n = 20) u skupini od ukupno 40 akaraman (bijeli karaman) ovaca, u dobi od jedne do dvije godine, činile su pokusnu skupinu bolesnih jedinki. Preostale ovce (n = 20) činile su kontrolnu skupinu zdravih jedinki. Tekućina buraga od svih pretraženih životinja dobivena je sondiranjem i odmah analizirana. Uzorci krvi uzeti su iz jugularne vene te je iz njih izdvojen serum. Koncentracija serumskog cTnI mjerena je komercijalnim imunoenzimnim testom, sendvič postupkom. Aktivnosti serumskih enzima (ALT, AST, CK-MB i LDH) utvrđene su uporabom kliničkog biokemijskog autoanalizatora. Prosječna serumska koncentracija cTnI u kontrolnoj skupini ovaca bila je na razini 0,035 ± 0,015 ng/mL (raspon: 0,02 - 0,06 ng/mL). U skupini ovaca s acidozom utvrđeno je postojano povećanje koncentracije cTnI koja je dosegla razinu od 0,103 ± 0,080 ng/mL (raspon: 0,03 - 1,7 ng/mL) (P<0,0001). U pokusnoj skupini opaženo je povećanje i drugog srčanog markera CK-MB koji je u ovoj skupini iznosio 454,50 ± 191,88 U/I (raspon: 214 - 861 U/I), a u kontrolnoj 224,35 ± 83,33 U/I (raspon: 133 - 421 U/I) (P<0,0001). Također je ustanovljeno da su ovce s acidozom u odnosu na ovce kontrolne skupine imale povišene jetrene enzime LDH (P<0,001) i AST (P<0,001), odnosno ALT (P<0,01). Zaključeno je da ovce s acidozom imaju povećanu koncentraciju serumskog cTnI koji može poslužiti kao važan biljeg za prognozu bolesti

Evaluation of oxidative stress in sheep infected with Psoroptes ovis using total antioxidant capacity, total oxidant status, and malondialdehyde level

Introduction: The study aimed at evaluating oxidative stress using malondialdehyde (MDA), total antioxidant capacity (TAC), total oxidant status (TOS), and oxidative stress index (OSI) markers in sheep naturally infected with Psoroptes ovis (Acari)