Fine Mapping of Shoot Fly Resistance and Stay-Green QTLS on Sorghum Chromosome SBI-10

Sorghum [Sorghum bicolor (L.) Moench, 2n = 2x = 20] is the fifth most important cereal crop globally, and is grown primarily in arid and semi-arid conditions. Major biotic and abiotic constraints hampering sorghum production include shoot fly infestation during early stages of crop development (seedling establishment and early growth stages; but only present in the eastern hemisphere) and terminal drought stress during post-flowering growth stages. These two stresses can devastate the crop. Hence developing sorghum varieties with resistance for these two stresses is critical. In order to understand the genetic basis of sensitivity to these two stresses and to genetically dissect host plant resistance to shoot fly and tolerance to terminal drought stress, the following objectives were proposed for the present study: to develop an introgression-line cross-based fine-mapping population for morphological components of shoot fly resistance and for the stay-green mechanism of terminal drought tolerance previously mapped to sorghum chromosome SBI-10L; to fine-map the target traits by combining genotyping and phenotyping datasets of a selected recombinant sub-set of the fine-mapping population; to annotate functionally and characterize candidate genes identified in the target region; and to identify recombinant progenies with pyramided traits of interest

Metallurgical investigation of cracked Al–5.5Zn–2.5Mg–1.5Cu aluminium alloy valve

AbstractThe high strength aluminium alloy Al–5.5Zn–2.5Mg–1.5Cu (AA7075) is being widely used in realisation of aerospace components. A component ‘fill and vent valve’ used in liquid propulsion system was fabricated from AA 7075 forgings in T7352 temper condition, and subsequently undergone various functional tests, four years back. Recently, during dye penetrant test after proof pressure test at 525bar, a valve indicated presence of a crack. Detailed metallurgical investigation indicated that failure was caused by stress corrosion cracking

STANDARDIZED CREATIVE FIND METHOD FOR RELATIONAL INFORMATION

Stretching The Keyword Search Concept Towards Relational Information Is An Engaged Portion Of Study Within Database And Understanding Retrieval Community In The Last Few Years. Abundant Techniques Were Forecasted, However No Matter Several Guides There Remain Inadequate Consistency For Assessment Of Forecasted Search Techniques. Our Understanding With Conventional Techniques Of Search Techniques Submit That Random Evaluations That Can Come Into View Inside The Literature Aren't Enough. They Were According To Survey Of Existing Evaluations By Information Retrieval Community For Assessment Of Retrieval Systems. Our Earlier Efforts Have In Contrast Techniques Of Relational Keyword Search Regarding Search Efficiency Try Not To Imagine Runtime Performance. Inside Our Work We Submit Most Meticulous Assessment Of Empirical Performance Concerning Relational Keyword Search That Has Came Out Up To Now Inside The Literature. Modified From Numerous Evaluations That Have Been Reported In Literature, Ours Examine Overall, Finish-To-Finish Performance Of Techniques Concerning Relational Keyword Search. Unlike Several Evaluations That Can Come Into View Inside The Literature, Our Benchmark Utilize Reasonable Data Sets And Practical Queries To Look At The Different Tradeoffs Created In Fashion Of Search Techniques. It Is The First Effort To Combine Performance And Appearance Efficiency In Character In Particular Figures Of Search Techniques

PROVIDING LEADING SESSION MODEL IN RATING SCHEME

Inside the literature works since there are some related studies, like web ranking junk e-mail recognition, recognition of internet review junk e-mail additionally to mobile application recommendation, impracticality of recognition of ranking fraud for mobile programs remains under-investigated. For achieving from the crucial void, we advise to build up a ranking fraud recognition system intended for mobile programs. We submit an all-natural vision of ranking fraud while increasing your ranking fraud recognition system intended for mobile programs. It's extended by means of other domain created particulars for ranking fraud recognition. Inside the recommended system of ranking fraud recognition system for mobile programs, it's worth watching the whole evidences are acquired by means of modelling of programs ranking, rating and review behaviours completely through record ideas tests

SmallSat Solar Axion and Activity X-ray Imager (SSAXI)

Axions are a promising dark matter candidate as well as a solution to the strong charge-parity (CP) problem in quantum chromodynamics (QCD). We describe a new mission concept for SmallSat Solar Axion and Activity X-ray Imager (SSAXI) to search for solar axions or axion-like particles (ALPs) and to monitor solar activity of the entire solar disc over a wide dynamic range. SSAXI aims to unambiguously identify X-rays converted from axions in the solar magnetic field along the line of sight to the solar core, effectively imaging the solar core. SSAXI also plans to establish a statistical database of X-ray activities from Active Regions, microflares, and Quiet Sun regions to understand the origin of the solar corona heating processes. SSAXI employs Miniature lightweight Wolter-I focusing X-ray optics (MiXO) and monolithic CMOS X-ray sensors in a compact package. The wide energy range (0.5 - 6 keV) of SSAXI can easily distinguish spectra of axion-converted X-rays from typical X-ray spectra of solar activities, while encompassing the prime energy band (3 - 4.5 keV) of axion-converted X-rays. The high angular resolution (30 arcsec HPD) and large field of view (40 arcmin) in SSAXI will easily resolve the enhanced X-ray flux over the 3 arcmin wide solar core while fully covering the X-ray activity over the entire solar disc. The fast readout in the inherently radiation tolerant CMOS X-ray sensors enables high resolution spectroscopy with a wide dynamic range in a broad range of operational temperatures. SSAXI will operate in a Sun-synchronous orbit for 1 yr preferably near a solar minimum to accumulate sufficient X-ray photon statistics.Comment: 12 pages, 9 figures, 1 table, Presented at the SPIE Optics + Photonics Conference, August 2019, San Diego, C

Evaluation of QTLs for Shoot Fly (Atherigona soccata) Resistance Component Traits of Seedling Leaf Blade Glossiness and Trichome Density on Sorghum (Sorghum bicolor) Chromosome SBI-10L

Shoot fly is a major insect pest of sorghum damaging early crop growth, establishment and productivity. Host plant resistance is an efficient approach to minimize yield losses due to shoot fly infestation. Seedling leaf blade glossiness and trichome density are morphological traits associated with shoot fly resistance. Our objective was to identify and evaluate QTLs for glossiness and trichome density using- i) 1894 F2s, ii) a sub-set of 369 F2-recombinants, and iii) their derived 369 F2:3 progenies, from a cross involving introgression lines RSG04008-6 (susceptible) × J2614-11 (resistant). The QTLs were mapped to a 37–72 centimorgan (cM) or 5–15 Mb interval on the long arm of sorghum chromosome 10 (SBI-10L) with flanking markers Xgap001 and Xtxp141. One QTL each for glossiness (QGls10) and trichome density (QTd10) were mapped in marker interval Xgap001-Xnhsbm1044 and Xisep0630-Xtxp141, confirming their loose linkage, for which phenotypic variation accounted for ranged from 2.29 to 11.37 % and LOD values ranged from 2.03 to 24.13, respectively. Average physical map positions for glossiness and trichome density QTLs on SBI-10 from earlier studies were 4 and 2 Mb, which in the present study were reduced to 2 Mb and 800 kb, respectively. Candidate genes Glossy15 (Sb10g025053) and ethylene zinc finger protein (Sb10g027550) falling in support intervals for glossiness and trichome density QTLs, respectively, are discussed. Also we identified a sub-set of recombinant population that will facilitate further fine mapping of the leaf blade glossiness and trichome density QTLs on SBI-10

Full-Shell X-Ray Optics Development at NASA Marshall Space Flight Center

NASAs Marshall Space Flight Center (MSFC) maintains an active research program toward the development of high-resolution, lightweight, grazing-incidence x-ray optics to serve the needs of future x-ray astronomy missions such as Lynx. MSFC development efforts include both direct fabrication (diamond turning and deterministic computer-controlled polishing) of mirror shells and replication of mirror shells (from figured, polished mandrels). Both techniques produce full-circumference monolithic (primary + secondary) shells that share the advantages of inherent stability, ease of assembly, and low production cost. However, to achieve high-angular resolution, MSFC is exploring significant technology advances needed to control sources of figure error including fabrication- and coating-induced stresses and mounting-induced distortions

Biotechnological Approaches to Evolve Sorghum (Sorghum bicolor (L.) Moench) for Drought Stress Tolerance and Shoot fly Resistance

Sorghum is a model tropical grass that uses C4 photosynthetic activity. But its yield is affected by many abiotic stresses likeheat, drought, cold, salt and also biotic stresses such as shoot fly, midges, and stem borerfromseedling stages to maturity. This article summarizes the terminal drought stress tolerance mechanism with staygreen phenotype expression during postflowering and also mechanisms of early shoot fly resistance during seedling stages of crop growth. The trait stay-green is extensively studied and its correlation to yield makes the stay-green trait more special for research and in marker assisted back cross programs. Under terminal drought stress conditions, stay-green trait is expressed with a complex mechanism involving many transcription factors, chlorophyll retention and nitrogen remobilization from leaves to maintain longer photosynthetic activity. Shoot fly resistance on the other hand, involves manyphysico-chemical, biologicaland morphological traits. Out of the many morphological traits, seedling leaf blade glossiness and trichome density are well characterized at genetic level and can assist as shoot fly resistance sources in marker-assisted breeding programs as they are highly negatively correlated with shoot fly dead heart formation. However, quantitative trait loci (QTL) mapping studies and candidate genes identified for the stay-green and shoot fly component traits need to be further validated with fine mapping, gene cloning and expression level studies. Pyramiding these two traits into a high yielding sorghum variety may lead to multiple stress resistance which could ultimately benefit the marginal farmers in India

Fine genetic mapping of combined shoot fly resistance (SFR) and stay green (STG) traits on sorghum chromosome SBI-10

Sorghum is fifth most important C4 cereal crop used as food, feed, fodder, fuel and a “fail safe” source in semi-arid tropics of the world..

Fine mapping of stay-green QTLs on sorghum chromosome SBI-10L ‒ An approach from genome to phenome

Sorghum is the fifth most important C4 cereal crop grown globally in arid and semi-arid climatic conditions. Drought is the major cause for loss of productivity worldwide. Delayed senescence of plants leads to adaptation to drought stress conditions by staying-green and giving high yields. In order to identify and dissect the stay-green genomic regions we have developed a high resolution fine mapping population from introgression line cross RSG04008-6 (stay-green) × J2614-11 (shoot fly resistant). Nearly 1894 F2 genotypes were screened with 8 SSR in order to identify double recombinants for both the parents in the sorghum chromosome-10 long arm (SBI-10L). The selected F2:4 recombinants were GBSed to increase the marker density between flanking markers Xgap001- Xtxp141 for stay-green QTLs on SBI-10L and a high resolution linkage map was developed using GBS SNPs. Out of 182 only 152 recombinants were replicated thrice in field for staygreen screening for two seasons (Summer 2013 and 2014). Fine mapping of the per cent Green Leaf Area (%GLA) traits identified 33 QTLs and 19QTLs were clustered into 7groups where 8 genes were identified. These were AP2/ERF transcription factor family (Sb10g025053), Ankyrin-repeat protein (Sb10g025310), WD40 repeat protein (Sb10g025320), NBSLRR Protein (Sb10g025283), Calcium dependant protein kinase (Sb10g030150), LEA2 protein (Sb10g029570), a putative uncharacterized protein (Sb10g024920) and senescence associated protein (Sb10g030520). With this study SBI-10L staygreen genomic regions were delimited from 15Mb to 8 genes co-localized with GWAS MTAs. Further cloning and expression level studies of the identified candidate genes will improve the development of drought tolerant genotypes