TOPICAL ANTI-PSORIATIC NANOPARTICULATE DRUG DELIVERY SYSTEM

Objective: Development of effective drug delivery in the treatment of psoriasis is the major challenge for its successful management. To develop and assess the potential of Nanostructured Lipid Carriers (NLCs) enriched with the powdered leaves extracts of Azadirachta indica (AE), Lawsonia inermis (LE) and fruit extract of Mallotus philippensis (ME) in the management of psoriasis. Methods: Drug loaded NLCs were prepared via hot homogenization technique by adopting 23 factorial design with factors X1 as the concentration of lipids, X2 concentration of surfactants and X3 being the number of homogenization cycle. The responses Y1 and Y2 were particle size and zeta potential. The optimized batch was obtained from Surface response plot and was evaluated for zeta potential, % entrapment efficiency, % drug loading, Scanning Electron Microscopy(SEM), % in vitro diffusion of drugs from the NLCs, anti-lipid peroxidation and nitric oxide scavenging activities, cytotoxicity on HaCat cell lines, Mouse Tail and Rat ultraviolet ray B photodermatitis models for Psoriasis. Results: The optimized batch of NLCs was found within the nanosized range with a relatively low polydispersity index and zeta potential of-20mV. The %EE for an optimized batch of NLCs was found to be 98.97±0.83%, 96.99±0.56% and 99.25±0.55% and the %DL of 21.84±0.15%, 8.55±045%, and 87.91±0.38% respectively for AE, LE and ME. The SEM images showed the spherical vesicular structures of drugs loaded NLCs. The in-vitro diffusion of drugs from the NLCs followed initial burst release thereafter sustained release for 24 h. The AE, LE and ME loaded NLCs proved to possess anti-lipid peroxidation and nitric oxide scavenging activities, cytotoxicity on HaCat cell lines, DNA fragmentation on HaCat cell lines which are biomarkers in the pathogenesis of psoriasis. The results of Mouse Tail and Rat ultraviolet ray B photodermatitis models for Psoriasis supported the anti-psoriatic potential of AE, LE and ME loaded NLCs. Conclusion: AE, LE and ME loaded NLCs can be used for prolonged topical delivery to the psoriatic skin for an effective treatment

DEVELOPMENT AND CHARACTERIZATION OF TOPICAL NANOPARTICULATE ANTIPSORIATIC POLYHERBAL CREAM

Objective: The lack of possible cure and associated disadvantages of allopathic medicines in the topical treatment of psoriasis has led to extensive research for anti-psoriatic activities of plant-based drugs. The present study was aimed to develop topical cream formulation containing the Nanostructured Lipid Carriers of Azadirachta indica leaves extract (AE), Lawsonia inermis leaves extract (LE) and fruit extract of Mallotus philippensis (ME) and assessing the antipsoriatic activity of prepared cream formulation. Methods: The Drug loaded NLCs were prepared via hot homogenization technique and incorporated into the water in oil (w/o) cream base that was prepared by the emulsification method. Evaluation of cream formulation included advanced preclinical trials using Rat Ultraviolet Ray-B photodermatitis and Mousetail method, anti-lipid peroxidation assay, nitric oxide scavenging activity, cytotoxicity and DNA fragmentation on HaCat cell lines to ascertain antipsoriatic potential and in vitro drug diffusion. Results: In spite of the low amount of the drug loading in NLCs their potency was much higher as displayed in Advanced Preclinical studies and cell line studies for psoriasis indicating the suitability of NLCs loaded creams for skin applications due to their various desirable effects on the skin. Occlusive properties of the prepared NLC on the skin provide an increase in drug penetration particularly via skin moisturization. The in vitro drug diffusion studies suggest the prolonged and almost complete release of AE, LE and ME from NLC based cream up to 24 h that was found to be 81.24±0.51%, 74.31±0.25% and 70.43±0.78% respectively. The literature survey supports the novelty of such topical anti-psoriatic polyherbal nanoparticulate cream. Conclusion: It can be concluded that the AE, LE and ME loaded NLC based cream can be used for prolonged topical delivery of drugs for the management of Psoriasis

FORMULATION AND DEVELOPMENT OF FENOFIBRATE LOADED LIPOSPHERE SYSTEM

Lipospheres offers a new approach to improve the solubility of poorly soluble drug. Fenofibrate is a third-generation fibric acid derivative belonging to BCS class-II, employed clinically as a hypolipidemic agent to lessen the risk caused by atherosclerosis. An attempt was made to improve aqueous solubility of FNO by aid of stearic acid and Paraffin oil.  The liposphere of FNO was prepared by melt dispersion technique using ultra turrax with %yield of 38% to 46% followed by their evaluation for saturation solubility, IR spectra, DSC, in-vitro study. Saturation solubility of FNO (92µg/ml) had improved to 184.31µg/ml with physical mixture of stearic and paraffin oil. Therefore, lipospheres of FNO were prepared using melt dispersion technique. The factorial batches were formulated using 32 factorial design with variables X1- concentration of stearic acid and X2- concentration of paraffin oil and responses Y1 - % Drug Entrapment (%DE) and Y2 - % Drug Release (% DR). The optimized batch was formulated and evaluated for Saturation Solublity, % DR, Invivo Study Thus from the present study it can be concluded that solubility of BCS class-II drugs can be improved by liposphere system. aid } �tapf�pÍ©and Paraffin oil.  The liposphere of FNO was prepared by melt dispersion technique using ultra turrax with %yield of 38% to 46% followed by their evaluation for saturation solubility, IR spectra, DSC, in-vitro study. Saturation solubility of FNO (92µg/ml) had improved to 184.31µg/ml with physical mixture of stearic and paraffin oil. Therefore, lipospheres of FNO were prepared using melt dispersion technique. The factorial batches were formulated using 32 factorial design with variables X1- concentration of stearic acid and X2- concentration of paraffin oil and responses Y1 - % Drug Entrapment (%DE) and Y2 - % Drug Release (% DR). The optimized batch was formulated and evaluated for Saturation Solublity, % DR, Invivo Study Thus from the present study it can be concluded that solubility of BCS class-II drugs can be improved by liposphere system.Keyword: Fenofibrate, Melt dispersion Technique, Liposphere.Â

AN OPEN-LABEL RANDOMIZED CLINICAL STUDY OF TOPICAL NANOPARTICULATE ANTIPSORIATIC POLYHERBAL CREAM

Objective: Objective of the study was to investigate the safety, efficacy, and antipsoriatic activity of topical cream enriched with Nanostructured Lipid Carriers (NLC) of Azadirachta indica, Lawsonia inermis, and Mallotus philippensis through the controlled clinical study. Methods: The randomized controlled trial was performed for 12 months on 65 adult patients between the age group 20 and 60 years of either sex of diagnosed, uncomplicated cases of psoriasis Vulgaris. The Test group received a local application of the herbal antipsoriatic NLC enriched cream, while the Control group was treated with Clobetasol propionate 0.05% cream twice daily for 3 months. Both groups were assessed for parameters of skin and nail examinations, lab investigation, and Psoriasis Area and Severity Index (PASI) score. The data were analyzed and interpreted statistically. Results: Significant improvement in itching and PASI score was seen during successive visits in both groups. However, recurrence of mild itching and erythema in a few patients of the control group was seen after 2 weeks, whereas no such recurrence is seen in the test group. Statistically significant reduction in eosinophilia in the control group was observed before and after treatment. The efficacy of clobetasol in the control group and the prepared formulation in the test group both show statistical efficacy at par. Conclusion: The data suggest that the NLC enriched cream exhibited significant relief in all the symptoms of psoriasis and therefore can be used as a potent antipsoriatic agent due to the easy availability of the drugs and cultural affinity for herbal formulations

DESIGN AND DEVELOPMENT OF CONTROLLED POROSITY OSMOTIC TABLETS OF GARCINIA INDICA EXTRACT

The present study deals with the development and evaluation of controlled porosity osmotic pump (CPOP) tablets containing inclusion complexes of ethanolic extract of Garcinia indica fruit rind. Wet granulation method was used for the development of core tablets. Core tablets were incorporated with different concentrations of sodium chloride as osmogen and additives. The CPOP tablets were coated with cellulose acetate as a wall forming material and HPMC acts as pore forming material in SPM. The formulated tablets were evaluated for FTIR, DSC, pre-compression parameters, post compression parameters, in vitro drug release study and scanning electron microscopy study. The optimized formulation had no significant effect on the pH and agitation intensity. SEM images revealed that no pores were found before dissolution and after dissolution had shown the porous nature of the membrane. It was found that the optimized formulation (P4) delivers a drug at a zero-order rate for 24 hours. Short term stability study at 40±2ºC /75±5% RH for the months on the optimized formulation indicated that there was no significant change in weight variation, % friability, drug content and in vitro drug release. Keywords: Osmotic system, cellulose acetate, pore former, controlled porosity, Garcinia indic

Formulation and development of antipsoriatic herbal gelcream

279-284This study presents formulation and development of patient friendly antipsoriatic topical gelcreams using Simulgel NS and Sepicide HB with Myrrh oil. Optimized formulation F5 depicted that gelcream had a viscoelastic nature with good creep recovery, and non-Newtonian behavior. Stability studies for three months at 40± 2oC and 75±5% RH of Formulation F5 showed no change in colour and consistency, and pH remained between 5.7 and 5.9. Primary skin irritation test conducted on 30 healthy volunteers demonstrated nonirritating nature of F5. Thus Simulgel NS and Sepicide HB (conc., 2% w/v each) were found to give better anti-inflammatory gelcream containing myrrh oil with good consistency and stability

A Study on Pulmonary Function Test among construction Workers

Introduction: The lungs are the only vital organ in direct contact with the external environment. Its function is to provide oxygen to the blood and remove carbon dioxide. The air passes through the trachea, bronchi and bronchioles and then finally reaches the alveoli. The occupation related lung diseases are more likely due to the deposition of dust in lungs and period of exposure, concentration and size of the airborne dust particles in the breathing zone. Methodology: This cross-sectional study was done among 120 construction workers compared with 60 controls to assess the respiratory illness, lung functions.This cross-sectional study was done at Construction sites in and around the area of Tertiary care teaching College and Hospital, and approved by institutional ethical committee. The present study was done in 120 participants (study group n = 60, Control group n = 60) selected by simple random technique.Results: The present study was done in 120 participants (study group-n=60, Control group n= 65). In our study, the most of workers age group is 18-30 years i.e., 37 out of 60 and in control group 18-30 years i.e., 39 out of 60. In study group out of 60, 42 subjects were male and 18 were female subjects. In control group 43 were male and 17 were female subjects. The Mean age of participants of study group is 33.7±7.14 and that of control group 36.23±5.2. The Mean weight of participants of study group is 57.2±08 and their mean height is 154±6.2. The Mean weight participants of control group is 59±8.1 and their mean height is 159.4±4.6. All the parameters of Pulmonary Function Test were significantly decreased (p < 0.0001) in study group as compare to control group. The prevalence of various respiratory symptoms in study and control group. Respiratory symptoms were more common among the study group as compare to control group. Overall prevalence of respiratory symptoms among study group more which is statistically significant when compared with control group.Conclusion: The present study adds evidence that cement dust adversely affects the respiratory functions and this impairment is association with duration of exposure to cement dust. Thus, this study showed existing changes in pulmonary function related to dust exposure, and generated evidence to integrate primary prevention methods towards dust- related morbidity and mortality