Combined Application of 17β-Estradiol and Progesterone Enhance Vascular Endothelial Growth Factor and Surfactant Protein Expression in Cultured Embryonic Lung Cells of Mice

Preterm delivery is associated with disruption of the placental supply with 17β-estradiol (E2) and progesterone (P). The aim is to evaluate the role of E2 and P on the regulation of key proteins in lung development in embryonic lung cells. Alveolar cell type II (AT-II) and central lung fibroblast cultures were established from mouse embryos. Cells were exposed for 24 hours to E2 and/or P, the estrogen receptor antagonist ICI 182.780 (ICI) and the progesterone receptor antagonist mifepristone (RU 486). The mRNA expression of vascular endothelial growth factor (VEGF) and surfactant protein B and C (SB-B, SB-C) was determined, and protein levels of VEGF were measured. Only the combined treatment with E2 and P increased mRNA expression and VEGF protein in AT-II cells and lung fibroblasts. Combined treatment also promoted SP-B and SP-C expression in AT-II cells. Pretreatment with ICI and RU 486 completely abolished the E2 and P induced effects. E2 and P enhanced expression of VEGF and surfactant proteins in primary embryonic lung cells and may be involved in regulating expression of key molecules for the prenatal lung development and postnatal lung function

Superhelasticity in aged single crystals of FeMnAlNi alloy

На [112]-монокристаллах Fe[43,5]Mnx[34]Al[15]Ni[7,5] показано, что при старении при T=437 K, 3 h наблюдается высокотемпературная сверхэластичность при деформации растяжением в температурном интервале от 298 K до 473 K. Экспериментально установлено, что механические и функциональные свойства зависят от ориентации кристалла, которая определяет вклад деформации раздвойникованием [epsilon][detw] в теоретическую величину деформации решетки

Factors impeding innovation. large and medium enterprises of Tomsk region in the period 2006-2016

The article analyzes the importance of factors impeding innovation of large and mediumsized enterprises of the Tomsk region from 2006 to 2016. The federal and regional statistical data are used for the analysis

The Liebherr Intelligent Hydraulic Cylinder as building block for innovative hydraulic concepts

We present hereafter the development of the Liebherr Intelligent Hydraulic Cylinder, in which the hydraulic component is used as smart sensing element providing useful information for the system in which the cylinder is operated. The piston position and velocity are the most important signals derived from this new measuring approach. The performance under various load and temperature conditions (measured both on dedicated test facilities and in field in a real machine) will be presented. An integrated control electronics, which is performing the cylinder state processing, additionally allows the synchronized acquisition of external sensors. Providing comprehensive state information, such as temperature and system pressure, advanced control techniques or monitoring functions can be realized with a monolithic device. Further developments, trends and benefits for the system architecture will be briefly analyzed and discussed

Intra-abdominal adipose tissue of laboratory mice adapted to different temperature regimes

For the first time the presence of beige adipocytes, cellularity, basic metabolic parameters of perigonadal abdominal fat have studied in autbred laboratory mice kept at different temperature regimes: 1) 30°С (thermoneutral zone) and 2) regular daily 8-hour cold exposures. Unlike brown fat in the abdominal depot, temperature-dependent changes of these parameters were not detected. The functions of the beige adipocytes of the abdominal depot were discussed