Authorship Diversity in General Surgery Related Cochrane Systematic Reviews

Background This study sought to determine the gender and country diversity in authorship representation in the authorship of Cochrane systematic reviews related to General Surgery.   Methods We searched and extracted data from the Cochrane Library on 3 September 2022 using ‘keyword:General surgery’, and included published reviews, protocols, and withdrawn publications. We extracted authors’ details and searched online to determine their gender, attempting to capture at least one webpage demonstrating it. Authors whose gender could not be ascertained were excluded from gender-based analyses. For graphical representation, we used a choropleth-style map. We treated a collaborative author group belonging to a single country, e.g., MRC Clinical Trials Unit (UK), as a single author. A second author independently cross-verified the extracted data.   Result Two hundred and fifty publications with a total of 1420 authors were included in the current study. Four authors had affiliation to two countries. The leading five represented nations (Figure 1A) in authorship were United Kingdom (n=562, 39.4%), China (n=163, 11.5%), Italy (n=144, 10.1%), Canada (n=91, 6.4%), and United States of America (n=89, 6.2%).   Syria is the only country among all the low-income countries which had authorship representation and constituted 0.34% (n=5) of all the authors. India (n=8, 0.6%) and Nigeria (n=2, 0.1%) were the only countries from lower-middle income groups who had representation. Male (n=957) to female (n=453) ratio in this study was 2.11:1 (Figure 1B). Sex data for ten authors couldn’t be retreived and were categorized as ‘unknown’ group.  There were 169 (67.3%) male and 82 (32.6%) female first authors (sex ratio 2.06:1). One study had designated two authors as co-first authors. Women (n= 81) constituted 32.4% of all the corresponding authors (sex ratio 2.06:1). One article didn’t have any designated corresponding author. One hundred and fifty (60%) studies didn’t have any female representation in any lead author (corresponding or first author) position. Fifty-eight (23.2%) studies didn’t have any female authors at all, whereas in contrast there were only eight studies (3.2%) which did not have any male authors.   Conclusion Authors from high-income countries continue to be the largest contributors to Cochrane systematic reviews in General Surgery, source of one of the highest quality evidence. There is extremely poor representation of female authors and authors from low and low-middle-income countries. Active capacity-building efforts are needed in several countries for advancing authorship diversity

Premature termination of transcription is shaped by Rho and translated uORFS in Mycobacterium tuberculosis

Little is known about the decisions behind transcription elongation versus termination in the human pathogen Mycobacterium tuberculosis (M.TB). By applying Term-seq to M.TB we found that the majority of transcription termination is premature and associated with translated regions, i.e., within previously annotated or newly identified open reading frames. Computational predictions and Term-seq analysis, upon depletion of termination factor Rho, suggests that Rho-dependent transcription termination dominates all transcription termination sites (TTS), including those associated with regulatory 5′ leaders. Moreover, our results suggest that tightly coupled translation, in the form of overlapping stop and start codons, may suppress Rho-dependent termination. This study provides detailed insights into novel M.TB cis-regulatory elements, where Rho-dependent, conditional termination of transcription and translational coupling together play major roles in gene expression control. Our findings contribute to a deeper understanding of the fundamental regulatory mechanisms that enable M.TB adaptation to the host environment offering novel potential points of intervention

A novel regulatory interplay between atypical B12 riboswitches and uORF translation in Mycobacterium tuberculosis

Vitamin B12 is an essential cofactor in all domains of life and B12-sensing riboswitches are some of the most widely distributed riboswitches. Mycobacterium tuberculosis, the causative agent of tuberculosis, harbours two B12-sensing riboswitches. One controls expression of metE, encoding a B12-independent methionine synthase, the other controls expression of ppe2 of uncertain function. Here, we analysed ligand sensing, secondary structure and gene expression control of the metE and ppe2 riboswitches. Our results provide the first evidence of B12 binding by these riboswitches and show that they exhibit different preferences for individual isoforms of B12, use distinct regulatory and structural elements and act as translational OFF switches. Based on our results, we propose that the ppe2 switch represents a new variant of Class IIb B12-sensing riboswitches. Moreover, we have identified short translated open reading frames (uORFs) upstream of metE and ppe2, which modulate the expression of their downstream genes. Translation of the metE uORF suppresses MetE expression, while translation of the ppe2 uORF is essential for PPE2 expression. Our findings reveal an unexpected regulatory interplay between B12-sensing riboswitches and the translational machinery, highlighting a new level of cis-regulatory complexity in M. tuberculosis. Attention to such mechanisms will be critical in designing next-level intervention strategies

The General Impact Of White Collar Crime: Reference To India And Kenya

White collar crime is a disease that frankly speaking is increasing at an alarming rate all across the globe. While the numbers of white collar crime increase, the impact it leaves behind down the line is astonishingly high after their ripple effect. First the impact of white collar crime to economy of the country is destroyed immensely leaving behind an economy that burdens the citizens who are trying to make end meet. Secondly, Political impact of these crimes on the other hand gives rise to civil unrests and protests perpetuated by citizens to their government. Leaving behind a state of political instability and a nation that harbors mistrust towards the governments. Thirdly, the impact it leaves towards the social fabric is astoundingly devastating, because it leads to lawlessness, increased crime rates and a culture of corruption in the country; which later becomes an accepted way of life. Fourth is the Impact it has on the environment; this is evident with the high cases of adulterated food in the market that poses a health problem to its consumers and pollution to the environment. Lastly is the impact on various professions, with reference to India and Kenya

The Mycobacterium tuberculosis sRNA F6 Modifies Expression of Essential Chaperonins, GroEL2 and GroES.

Almost 140 years after the identification of Mycobacterium tuberculosis as the etiological agent of tuberculosis, important aspects of its biology remain poorly described. Little is known about the role of posttranscriptional control of gene expression and RNA biology, including the role of most of the small RNAs (sRNAs) identified to date. We have carried out a detailed investigation of the M. tuberculosis sRNA F6 and shown it to be dependent on SigF for expression and significantly induced in starvation conditions in vitro and in a mouse model of infection. Further exploration of F6 using an in vitro starvation model of infection indicates that F6 affects the expression of the essential chaperonins GroEL2 and GroES. Our results point toward a role for F6 during periods of low metabolic activity typically associated with long-term survival of M. tuberculosis in human granulomas. IMPORTANCE Control of gene expression via small regulatory RNAs (sRNAs) is poorly understood in one of the most successful pathogens, Mycobacterium tuberculosis. Here, we present an in-depth characterization of the sRNA F6, including its expression in different infection models and the differential gene expression observed upon deletion of the sRNA. Our results demonstrate that deletion of F6 leads to dysregulation of the two essential chaperonins GroEL2 and GroES and, moreover, indicate a role for F6 in the long-term survival and persistence of M. tuberculosis in the human host

The mycobacterium tuberculosis sRNA F6 modifies expression of essential chaperonins, GroEL2 and GroES

Almost 140 years after the identification of Mycobacterium tuberculosis as the etiological agent of tuberculosis, important aspects of its biology remain poorly described. Little is known about the role of posttranscriptional control of gene expression and RNA biology, including the role of most of the small RNAs (sRNAs) identified to date. We have carried out a detailed investigation of the M. tuberculosis sRNA F6 and shown it to be dependent on SigF for expression and significantly induced in starvation conditions in vitro and in a mouse model of infection. Further exploration of F6 using an in vitro starvation model of infection indicates that F6 affects the expression of the essential chaperonins GroEL2 and GroES. Our results point toward a role for F6 during periods of low metabolic activity typically associated with long-term survival of M. tuberculosis in human granulomas. IMPORTANCE Control of gene expression via small regulatory RNAs (sRNAs) is poorly understood in one of the most successful pathogens, Mycobacterium tuberculosis. Here, we present an in-depth characterization of the sRNA F6, including its expression in different infection models and the differential gene expression observed upon deletion of the sRNA. Our results demonstrate that deletion of F6 leads to dysregulation of the two essential chaperonins GroEL2 and GroES and, moreover, indicate a role for F6 in the long-term survival and persistence of M. tuberculosis in the human host

International Perspectives on Nonformal Education

The New England Regional Meeting of the Comparative and International Education Society was held on the campus of the University of Massachusetts, Amherst, on 3 May, 1979. The conference was co-sponsored by the Division of Community Education, Springfield College, and the Center for International Education, School of Education, University of Massachusetts. The theme of the conference was International Perspectives on Nonformal Education. The papers delivered ranged in topics from the use of traditional art forms and poetry as a medium of nonformal education to the discussion of the philosophical foundations underlying the field. Case studies of specific nonformal education projects helped to highlight the conference. A total fifteen papers were delivered, all of which are included in their original form in this document. The papers represent the views of the authors and not necessarily those of the organizers