Alternatives for jet engine control

The development of models of tensor type for a digital simulation of the quiet, clean safe engine (QCSE) gas turbine engine; the extension, to nonlinear multivariate control system design, of the concepts of total synthesis which trace their roots back to certain early investigations under this grant; the role of series descriptions as they relate to questions of scheduling in the control of gas turbine engines; the development of computer-aided design software for tensor modeling calculations; further enhancement of the softwares for linear total synthesis, mentioned above; and calculation of the first known examples using tensors for nonlinear feedback control are discussed

Factors Affecting the Distribution of Yeasts in the Antarctic Ross Desert

This thesis is comprised of four main parts--Chapters I, II, III, and an Appendix. The first chapter contains the Introduction and Literature Search. The remaining two chapters are manuscripts in preparation to be submitted to the Journal of General Microbiology. Chapter II is "A Comparison of Yeasts from Arid and Well-Watered Ross Desert Sites," and is a paper in preparation as "The morphology of Antarctic yeasts is correlated with aridity." This title reflects the conclusions of identifying yeasts from a well watered habitat (glacial melt stream) and comparisons with those found (as described species or biovars) in arid soil. Chapter III in this thesis is "The Effect of Soil Mineral Salts on Yeast Distribution in the Ross Desert," and is a paper in preparation as "Soil parameters limiting the distribution of yeasts in the Ross Desert." The Appendix describes in greater detail the various materials and methods referred to only briefly in the manuscripts. Approval for presenting this thesis in the above manner is based upon the Graduate College's policy of accepting a thesis in manuscript form and is subject to the Graduate College's approval of the major professor's request for a waiver of the standard format which will be submitted in March 1987.Microbiolog

The iBeetle large-scale RNAi screen reveals gene functions for insect development and physiology

Genetic screens are powerful tools to identify the genes required for a given biological process. However, for technical reasons, comprehensive screens have been restricted to very few model organisms. Therefore, although deep sequencing is revealing the genes of ever more insect species, the functional studies predominantly focus on candidate genes previously identified in Drosophila, which is biasing research towards conserved gene functions. RNAi screens in other organisms promise to reduce this bias. Here we present the results of the iBeetle screen, a large-scale, unbiased RNAi screen in the red flour beetle, Tribolium castaneum, which identifies gene functions in embryonic and postembryonic development, physiology and cell biology. The utility of Tribolium as a screening platform is demonstrated by the identification of genes involved in insect epithelial adhesion. This work transcends the restrictions of the candidate gene approach and opens fields of research not accessible in Drosophila

<em>In vivo</em> imaging of MMP-13 activity using a specific polymer-FRET peptide conjugate detects early osteoarthritis and inhibitor efficacy.

Imaging early molecular changes in osteoarthritic (OA) joints is instrumental for the development of disease-modifying drugs. To this end, a fluorescent resonance energy transfer-based peptide probe that is cleavable by matrix metalloproteinase 13 (MMP-13) has been developed. This protease degrades type II collagen, a major matrix component of cartilage. The probe exhibits high catalytic efficiency (k(cat)/K-M = 6.5 x 10(5) m(-1) s(-1)) and high selectivity for MMP-13 over a set of nine MMPs. To achieve optimal in vivo pharmacokinetics and tissue penetration, the probe has been further conjugated to a linear l-polyglutamate chain of 30 kDa. The conjugate detects early biochemical events that occur in a surgically induced murine model of OA before major histological changes. The nanometric probe is suitable for the monitoring of in vivo efficacy of an orally bioavailable MMP-13 inhibitor, which effectively blocks cartilage degradation during the development of OA. This new polymer-probe can therefore be a useful tool in detecting early OA, disease progression, and in developing MMP-13-based disease-modifying drugs for OA