Gila River Flow Needs Assessment

The Nature Conservancy and a team of 14 academic partners (the project team) received funding from the Bureau of Reclamation’s WaterSMART program and the Desert Landscape Conservation Cooperative in 2012 to conduct this Gila River Flow Needs Assessment. The assessment describes the existing condition of the Gila River in the Cliff-Gila Valley and examines the potential impacts of CUFA diversion and climate change on the riparian and aquatic ecosystem. The project team was assisted by 35 academic, agency and consulting scientists who have expertise in some aspect of the Gila River’s hydrology and ecology. This larger team of scientists provided input on a review draft of this assessment at a workshop in January 2014. This assessment report includes 12 chapters written by project team scientists. Two chapters summarize workshop findings, including input of the larger team of scientists on how flows shape the ecosystem and how these interactions may be affected by flow alterations due to CUFA diversion and climate change

Antiproliferative Withanolides from Datura wrightii

A new withanolide, named withawrightolide (1), and four known withanolides (2−5) were isolated from the aerial parts of Datura wrightii. The structure of compound 1 was elucidated through 2D NMR and other spectroscopic techniques. In addition, the structure of withametelin L (2) was confirmed by X-ray crystallographic analysis. Using MTS viability assays, withanolides 1−5 showed antiproliferative activities against human glioblastoma (U251 and U87), head and neck squamous cell carcinoma (MDA-1986), and normal fetal lung fibroblast (MRC-5) cells with IC50 values in the range between 0.56 and 5.6 μM

Minor Withanolides of Physalis longifolia: Structure and Cytotoxicity

This is the publisher's version, also available electronically from https://www.jstage.jst.go.jp/article/cpb/60/10/60_c12-00305/_article.In our recent publication on bioactive guided isolation of compounds from Physalis longifolia (Solanaceae) novel anti-proliferative agents withalongolides A (4) and B (5), and their highly cytotoxic analogues, withalongolide A 4,19,27-triacetate (4a) and withalongolide B 4,19-diacetate (5a) were elucidated. In this study, the two lead compounds (4, 5) were re-isolated in gram quantities for the purpose of further analogue preparation and in vivo testing that would continue to probe structure–activity relationships. During this process, two additional withanolides, named withalongolides O (1) and P (2), were elucidated. Their structures were determined by spectroscopic techniques with 1 being subsequently confirmed by X-ray crystallographic analysis. Utilizing a MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] viability assay, withalongolide O (1) and its 4,7-diaceatate (1a), both containing the functionalities of Δ2-1-oxo- in A ring, a 5β,6β-epoxy in B ring, and a lactone ring in the nine-carbon side chain, exhibited potent cytotoxicity against human head and neck squamous cell carcinoma (JMAR and MDA-1986), melanoma (B16F10 and SKMEL-28), and normal fetal lung fibroblast (MRC-5) cells with IC50 values in the range between 0.15 and 2.95 µM. In addition, the previously reported α orientation of 7-acetate group in acnistins C and D should be revised to the β orientation on the basis of NMR data comparison