7 research outputs found
Imaging and Three-Dimensional Reconstruction of Chemical Groups in a Protein Complex using DNA Labels
Angstrom-Scale Chemical Microscopy: Multicolor Single-Molecule Imaging with Energy Landscape Engineering
Contrast simulation in the phase shift dynamic magnetic force microscopy depending on the MFM probe geometry and materials
Ferritin-Based New Magnetic Force Microscopic Probe Detecting 10 nm Sized Magnetic Nanoparticles
A single-molecule ferritin picking-up process was realized with the use of AFM, which was enhanced by employing controlled dendron surface chemistry. The approach enabled the placement of a single ferritin protein molecule at the very end of an AFM tip. When used for magnetic force microscopy (MFM) imaging, the tips were able to detect magnetic interactions of approximately 10 nm sized magnetic nanoparticles. The single ferritin tip also showed the characteristics of a “multifunctional” MFM probe that can sense the magnetic force from magnetic materials as well as detect the biomolecular interaction force with DNAs on the surface. The multifunctional tip enabled us not only to investigate the specific molecular interaction but also to image the magnetic interaction between the probe and the substrate, in addition to allowing the common capability of topographic imaging. Because the protein engineering of ferritin and the supporting coordination and conjugation chemistry are well-established, we envisage that it would be straightforward to extend this approach to the development of various single magnetic particle MFM probes of different compositions and sizes