Septins regulate junctional integrity of endothelial monolayers

Junctional integrity of endothelial monolayers is crucial to control movement of molecules and cells across the endothelium. Examining the structure and dynamics of cell junctions in endothelial monolayers, we discovered a role for septins. Contacts between adjacent endothelial cells were dynamic, with protrusions extending above or below neighboring cells. Vascular endothelial cadherin (VE-cadherin) was present at cell junctions, with a membrane-associated layer of F-actin. Septins localized at cell-junction membranes, in patterns distinct from VE-cadherin and F-actin. Septins assumed curved and scallop-shaped patterns at junctions, especially in regions of positive membrane curvature associated with actin-rich membrane protrusions. Depletion of septins led to disrupted morphology of VE-cadherin junctions and increased expression of VE-cadherin. In videos, septin-depleted cells displayed remodeling at cell junctions; regions with VE-cadherin were broader, and areas with membrane ruffling were wider. Septin depletion and junction disruption led to functional loss of junctional integrity, revealed by decreased transendothelial electric resistance and increased transmigration of immune cells. We conclude that septins, as cytoskeletal elements associated with the plasma membrane, are important for cell junctions and junctional integrity of endothelial monolayers, functioning at regions of positive curvature in support of actin-rich protrusions to promote cadherin-based cell junctions.</jats:p

Clyde tributaries : report of urban stream sediment and surface water geochemistry for Glasgow

This report presents the results of an urban drainage geochemical survey carried out jointly by the British Geological Survey (BGS) and Glasgow City Council (GCC) during June 2003. 118 stream sediment and 122 surface water samples were collected at a sample density of 1 per 1 km2 from all tributaries draining into the River Clyde within the GCC administrative area. The study was carried out as part of the BGS systematic Geochemical Surveys of Urban Environments (GSUE) programme. Stream sediment and surface water samples underwent analysis for approximately 46 chemical elements including contaminants such as As, Al, Cd, Cu, Cr, Ni, Pb, Se, V and Zn according to standard GSUE procedures. In addition, parameters such as ammonium, asbestos and Hg as well as organic contaminants such as total petroleum hydrocarbons (TPH), polycyclic aromatic hydrocarbons (PAH), poly-chlorinated biphenyls (PCB) and organo-tin compounds were assessed. The aim of the project was to provide an overview of urban drainage geochemistry in Glasgow to link to an on-going sister project, which is investigating the geochemistry of the Clyde estuary. This report presents the initial findings of the Clyde tributaries survey but it is envisaged that the data will be interpreted in more detail as part of a wider Clyde basin study once the Clyde estuary survey is completed

Capping protein binding to actin in yeast: biochemical mechanism and physiological relevance

The mechanism by which capping protein (CP) binds barbed ends of actin filaments is not understood, and the physiological significance of CP binding to actin is not defined. The CP crystal structure suggests that the COOH-terminal regions of the CP α and β subunits bind to the barbed end. Using purified recombinant mutant yeast CP, we tested this model. CP lacking both COOH-terminal regions did not bind actin. The α COOH-terminal region was more important than that of β. The significance of CP's actin-binding activity in vivo was tested by determining how well CP actin-binding mutants rescued null mutant phenotypes. Rescue correlated well with capping activity, as did localization of CP to actin patches, indicating that capping is a physiological function for CP. Actin filaments of patches appear to be nucleated first, then capped with CP. The binding constants of yeast CP for actin suggest that actin capping in yeast is more dynamic than in vertebrates

A Grand Canonical Ensemble Approach to the Thermodynamic Properties of the Nucleon in the Quark-Gluon Coupling Model

In this paper, we put forward a way to study the nucleon's thermodynamic properties such as its temperature, entropy and so on, without inputting any free parameters by human hand, even the nucleon's mass and radius. First we use the Lagrangian density of the quark gluon coupling fields to deduce the Dirac Equation of the quarks confined in the gluon fields. By boundary conditions we solve the wave functions and energy eigenvalues of the quarks, and thus get energy-momentum tensor, nucleon mass, and density of states. Then we utilize a hybrid grand canonical ensemble, to generate the temperature and chemical potentials of quarks, antiquarks of three flovars by the four conservation laws of the energy and the valence quark numbers, after which, all other thermodynamic properties are known. The only seemed free paremeter, the nucleon radius is finally determined by the grand potential minimal principle.Comment: 5 pages, LaTe

Finite Size Scaling and ``perfect'' actions: the three dimensional Ising model

Using Finite-Size Scaling techniques, we numerically show that the first irrelevant operator of the lattice $\lambda\phi^4$ theory in three dimensions is (within errors) completely decoupled at $\lambda=1.0$. This interesting result also holds in the Thermodynamical Limit, where the renormalized coupling constant shows an extraordinary reduction of the scaling-corrections when compared with the Ising model. It is argued that Finite-Size Scaling analysis can be a competitive method for finding improved actions.Comment: 13 pages, 3 figure

High speed jet noise research at NASA Lewis

The source noise portion of the High Speed Research Program at NASA LeRC is focused on jet noise reduction. A number of jet noise reduction concepts are being investigated. These include two concepts, the Pratt & Whitney ejector suppressor nozzle and the General Electric (GE) 2D-CD mixer ejector nozzle, that rely on ejectors to entrain significant amounts of ambient air to mix with the engine exhaust to reduce the final exhaust velocity. Another concept, the GE 'Flade Nozzle' uses fan bypass air at takeoff to reduce the mixed exhaust velocity and to create a fluid shield around a mixer suppressor. Additional concepts are being investigated at Georgia Tech Research Institute and at NASA LeRC. These will be discussed in more detail in later figures. Analytical methods for jet noise prediction are also being developed. Efforts in this area include upgrades to the GE MGB jet mixing noise prediction procedure, evaluation of shock noise prediction procedures, and efforts to predict jet noise directly from the unsteady Navier-Stokes equation