Novel Diagnostic Model for the Deficient and Excess Pulse Qualities

The deficient and excess pulse qualities (DEPs) are the two representatives of the deficiency and excess syndromes, respectively. Despite its importance in the objectification of pulse diagnosis, a reliable classification model for the DEPs has not been reported to date. In this work, we propose a classification method for the DEPs based on a clinical study. First, through factor analysis and Fisher's discriminant analysis, we show that all the pulse amplitudes obtained at various applied pressures at Chon, Gwan, and Cheok contribute on equal orders of magnitude in the determination of the DEPs. Then, we discuss that the pulse pressure or the average pulse amplitude is appropriate for describing the collective behaviors of the pulse amplitudes and a simple and reliable classification can be constructed from either quantity. Finally, we propose an enhanced classification model that combines the two complementary variables sequentially

Directional interacting whispering gallery modes in coupled dielectric microdisks

We study the optical interaction in a coupled dielectric microdisks by investigating the splitting of resonance positions of interacting whispering gallery modes (WGMs) and their pattern change, depending on the distance between the microdisks. It is shown that the interaction between the WGMs with odd parity about y-axis becomes appreciable at a distance less than a wavelength and causes directional emissions of the resulting interacting WGMs. The directionality of the interacting WGMs can be understood in terms of an effective boundary deformation in ray dynamical analysis. We also discuss about the oscillation of the splitting when the distance is greater than a wavelength.Comment: 7 pages, 10 figure

Human microglial cells synthesize albumin in brain

Albumin has been implicated in Alzheimer's disease since it can bind to and transport amyloid beta, the causative agent; albumin is also a potent inhibitor of amyloid beta polymerization. In a pilot phase study of Human Brain Proteome Project, we found evidence that albumin may be synthesized in immortalized human microglial cells, human primary microglial cells, and human fetal and adult brain tissues. We also found the synthesis and secretion is enhanced upon microglial activation by Amyloid [beta]~1-42~, lipopolysaccharide treatment or human Alzheimer's brain

Experimental study on the removal of sulfur compounds and siloxanes from biogas

Biogas is a renewable energy source, which can be produced by anaerobic digestion with anaerobic organisms from agriculture waste, manure, municipal waste, sewage, food waste, etc. The biogas consists primarily of methane and carbon dioxide, but also smaller amounts of nitrogen, oxygen, hydrogen and volatile organic compounds including sulfur compounds, halogenated compounds and organic silicon compounds may be present. Here, methane which is the main component in the biogas may be used as a fuel in many applications such as heating, combined heat and power systems, fuel cells, etc. For the implementation of methane, therefore, the biogas needs purification to improve its quality in most cases by removing impurities from the biogas, resulting in no corrosion and scaling problems in the applications. In the present work, a hybrid biogas purification process, consisting of a physicochemical process with an adsorption for the removal of sulfur compounds and siloxanes and a membrane separation process for the removal of carbon dioxide, has been proposed. The main focus of this study is to examine the physical properties and adsorption characteristics of adsorbents being used to remove sulfur compounds and siloxanes from the biogas. Indeed, recent studies are on the desulfurization and siloxane removal process using an activated carbon and impregnated activated carbon. However, there are many different types of sulfur compounds and siloxanes in the biogas and each may have a different reaction rate and adsorption capacity. In this study, therefore, several commercially-available adsorbents are selected to analyze their removal capacities for the main components (methane) and major impurities in the biogas. The main impurities considered in this work are hydrogen sulfide (H2S), carbonyl sulfide (COS), carbon disulfide (CS2), Octamethylcyclotetrasiloxane (D4) and Decamethylcyclopentasiloxane (D5), based on the measurements from the on-site sewage treatment plant in Incheon, Korea. In the bench-scale adsorption experiments, iron oxide, activated carbon, impregnated activated carbon and inorganic adsorbents such as zeolite and silica gel are used as adsorbents for the removal of impurities from synthetic biogas and their physical properties are analyzed with XRF, SEM and BET analyses. The experimental results show that the adsorption capacity of hydrogen sulfide in the iron oxide (IO) is superior to those of the activated carbon (AC) and impregnated activated carbon (IAC) with a relatively good adsorption capacity (Figure 1). In addition, the removal efficiency of carbonyl sulfide and carbon disulfide with the activated carbon is more effective than using the iron oxide having a very poor adsorption capacity (Figure 2). It is also shown that both activated carbon and zeolite exhibit a high adsorption capacity of siloxanes D4 and D5. Especially, in case of siloxane D5, the zeolite has a better adsorption capacity than the activated carbon (Figure 3). More detailed results will be presented at the conference. Please click Additional Files below to see the full abstract

Novel Diagnostic Algorithm for the Floating and Sunken Pulse Qualities and Its Clinical Test

We propose a novel classification algorithm for the floating pulse and the sunken pulse using a newly defined coefficient (Cfs). To examine the validity of the proposed algorithm, we carried out a clinical test in which 12 oriental medical doctors made pairwise diagnoses on the pulses of volunteering subjects. 169 subjects were simultaneously diagnosed by paired doctors, and the diagnoses in 121 subjects were concordant, yielding an accuracy of 72% and a Matthews correlation coefficient of 0.42, which indicates reasonable agreement between doctors. Two sample T-tests showed that subjects in the sunken pulse group had significantly higher BMI and Cfs (P < .05) than those in the floating pulse group. The pulse classification by the algorithm converged with the diagnoses of paired doctors with an accuracy up to 69%. With these results, we confirm the validity of the novel classification algorithm for the floating and sunken pulses

Akt regulates the expression of MafK, synaptotagmin I, and syntenin-1, which play roles in neuronal function

<p>Abstract</p> <p>Background</p> <p>Akt regulates various cellular processes, including cell growth, survival, and metabolism. Recently, Akt's role in neurite outgrowth has also emerged. We thus aimed to identify neuronal function-related genes that are regulated by Akt.</p> <p>Methods</p> <p>We performed suppression subtractive hybridization on two previously established PC12 sublines, one of which overexpresses the wild-type (WT) form and the other, the dominant-negative (DN) form of Akt. These sublines respond differently to NGF's neuronal differentiation effect.</p> <p>Results</p> <p>A variety of genes was identified and could be classified into several functional groups, one of which was developmental processes. Two genes involved in neuronal differentiation and function were found in this group. v-Maf musculoaponeurotic fibrosarcoma oncogene homolog K (MafK) induces the neuronal differentiation of PC12 cells and immature telencephalon neurons, and synaptotagmin I (SytI) is essential for neurotransmitter release. Another gene, <it>syntenin-1 </it>(<it>Syn-1</it>) was also recognized in the same functional group into which <it>MafK </it>and <it>SytI </it>were classified. Syn-1 has been reported to promote the formation of membrane varicosities in neurons. Quantitative reverse transcription polymerase chain reaction analyses show that the transcript levels of these three genes were lower in PC12 (WT-Akt) cells than in parental PC12 and PC12 (DN-Akt) cells. Furthermore, treatment of PC12 (WT-Akt) cells with an Akt inhibitor resulted in the increase of the expression of these genes and the improvement of neurite outgrowth. These results indicate that dominant-negative or pharmacological inhibition of Akt increases the expression of <it>MafK</it>, <it>SytI</it>, and <it>Syn-1 </it>genes. Using lentiviral shRNA to knock down endogenous Syn-1 expression, we demonstrated that Syn-1 promotes an increase in the numbers of neurites and branches.</p> <p>Conclusions</p> <p>Taken together, these results indicate that Akt negatively regulates the expression of <it>MafK</it>, <it>SytI</it>, and <it>Syn-1 </it>genes that all participate in regulating neuronal integrity in some way or another.</p

Zero mode in the time-dependent symmetry breaking of λϕ4\lambda\phi^4 theory

We apply the quartic exponential variational approximation to the symmetry breaking phenomena of scalar field in three and four dimensions. We calculate effective potential and effective action for the time-dependent system by separating the zero mode from other non-zero modes of the scalar field and treating the zero mode quantum mechanically. It is shown that the quantum mechanical properties of the zero mode play a non-trivial role in the symmetry breaking of the scalar $\lambda \phi^4$ theory.Comment: 10 pages, 3 figure

Carotid Endarterectomy for Symptomatic Complete Occlusion of the Internal Carotid Artery

We described 9 consecutive patients who underwent operative carotid artery exploration with attempted carotid endarterectomy (CEA) for symptomatic internal carotid artery (ICA) occlusion. Indications for this surgery based on vascular imaging included segmental occlusion of the proximal ICA and also extensive occlusion of the distal ICA in selected patients in whom color-flow duplex ultrasound showed a poorly echogenic or anechoic thrombus with a flow void, suggestive of an acute thrombus. CEA was performed successfully to restore blood flow in all 9 patients:CEA in 5 and CEA with Fogarty thrombectomy in 4. Postoperative magnetic resonance (MR) angiography confirmed that revascularization had been successful in all 9 patients, and MR imaging displayed improved perfusion in 4 patients. Despite the lack of a generalized efficacy of surgical revascularization for symptomatic ICA occlusion, our study demonstrated that preoperative vascular imaging allows the selection of patients who may benefit from CEA

A Clinical Study of the Pulse Wave Characteristics at the Three Pulse Diagnosis Positions of Chon, Gwan and Cheok

In this work, we analyze the baseline, signal strength, aortic augmentation index (AIx), radial AIx, time to reflection and P_T2 at Chon, Gwan, and Cheok, which are the three pulse diagnosis positions in Oriental medicine. For the pulse measurement, we used the SphygmoCor apparatus, which has been widely used for the evaluation of the arterial stiffness at the aorta. By two-way repeated measures analysis of variance, we tested two independent measurements for repeatability and investigated their mean differences among Chon, Gwan and Cheok. To characterize further the parameters that were shown to be different between each palpation position, we carried out Duncan's test for the multiple comparisons. The baseline and signal strength were statistically different (P < .05) among Chon, Gwan and Cheok, respectively, which supports the major hypothesis of Oriental medicine that all of the three palpation positions contain different clinical information. On the other hand, aortic AIx and time to reflection were found to be statistically different between Chon and the others, and radial AIx and P_T2 did not show any difference between pulse positions. In the clinical sense, however, the aortic AIx at each palpation position was found to fall within the 90% confidence interval of normal arterial compliance. The results of the multiple comparisons indicate that the parameters of arterial stiffness were independent of the palpation positions. This work is the first attempt to characterize quantitatively the pulse signals at Chon, Gwan and Cheok with some relevant parameters extracted from the SphygmoCor apparatus

Cross-genotype protection of live-attenuated vaccine candidate for severe fever with thrombocytopenia syndrome virus in a ferret model

Severe fever with thrombocytopenia syndrome (SFTS) virus (SFTSV) is an emerging tick-borne virus classified within the Banyangvirus genus. SFTS disease has been reported throughout East Asia since 2009 and is characterized by high fever, thrombocytopenia, and leukopenia and has a 12 to 30% case fatality rate. Due to the recent emergence of SFTSV, there has been little time to conduct research into preventative measures aimed at combatting the virus. SFTSV is listed as one of the World Health Organization’s Prioritized Pathogens for research into antiviral therapeutics and vaccine development. Here, we report 2 attenuated recombinant SFTS viruses that induce a humoral immune response in immunized ferrets and confer complete cross-genotype protection to lethal challenge. Animals infected with rHB29NSsP102A or rHB2912aaNSs (both genotype D) had a reduced viral load in both serum and tissues and presented without high fever, thrombocytopenia, or mortality associated with infection. rHB29NSsP102A- or rHB2912aaNSs-immunized animals developed a robust anti-SFTSV immune response against cross-genotype isolates of SFTSV. This immune response was capable of neutralizing live virus in a focus-reduction neutralization test (FRNT) and was 100% protective against a cross-genotype lethal challenge with the CB1/2014 strain of SFTSV (genotype B). Thus, using our midsized, aged ferret infection model, we demonstrate 2 live attenuated vaccine candidates against the emerging pathogen SFTSV