High energy-charged cell factory for heterologous protein synthesis

Overexpression of gluconeogenic phosphoenolpyruvate carboxykinase (PCK) under glycolytic conditions enables Escherichia coli to maintain a greater intracellular ATP concentration and, consequently, to up-regulate genes for amino acid and nucleotide biosynthesis. To investigate the effect of a high intracellular ATP concentration on heterologous protein synthesis, we studied the expression of a foreign gene product, enhanced green fluorescence protein (eGFP), under control of the T7 promoter in E. coli BL21(DE3) strain overexpressing PCK. This strain was able to maintain twice as much intracellular ATP and to express two times more foreign protein than the control strain. These results indicate that a high energy-charged cell can be beneficial as a protein-synthesizing cell factory. The potential uses of such a cell factory are discussed

Video-Based Stylized Rendering using Frame Difference

In this paper, we suggest video based stylized rendering using frame difference. Stylized rendering using video frame has a temporal problem that occurs a difference between the previous and current frame. To reduce the temporal problem, we generate reference maps using temporal frame difference in correction and rendering steps. A correction method using reference maps can be reduced flickering effect caused by frame difference between the previous and current frame. We use a background map, an average map, and a quadtree-based summed area table as reference maps. Among these reference maps, the method using quadtree based summed area table can completely remove a flickering and popping effect. Also, a post-blurring method using bilateral filtering can be represented smooth, stylized rendering by removing unnecessary noise. Suggested stylized rendering system can be used in various fields such as visual art, advertisement, game and movie for stylized image contents generation

Environmental considerations of plastic behaviors for automobile applications

AbstractIt is well known fact that the thermo-mechanical behaviors of polymeric materials are strongly influenced by environmental factors, and, for automobiles, the mechanical properties of interior plastic structures are noticeably changed by being repeatedly exposed to environments such as sun light and rains. As the properties change, mechanical fits such as fasteners and clips in automobiles lose their tightness, creating unexpected noises. To consider Buzz, Squeak and Rattle (BSR) from initial stage of the interior design, it is very important to obtain, analyze and understand the structural behaviors of the materials under environmental changes as well as time. In this report, the mechanical property changes of the plastics for automobiles are measured to investigate the temperature and humidity effects. The samples are undergone different temperature and humidity conditions, and regularly taken out to measure the thermo-mechanical properties. The data are compared with the original samples, and analyzed for the properties change. Viscoelastic characteristics such as glass transition temperatures and storage/loss modulus were also investigate

anti-9,10-Di(1-naphth­yl)anthracene pyridine disolvate

In the title compound, C34H22·2C5H5N, there is a crystallographic inversion center in the middle of the anthracene ring system. The dihedral angle between the mean planes of the anthracene and naphthalene ring systems is 83.96 (4)°. The crystal structure is stabilized by weak inter­molecular C—H⋯N and C—H⋯π inter­actions