Intervention Strategies Based on Information-Motivation-Behavioral Skills Model for Health Behavior Change: A Systematic Review

SummaryPurposeThis study systematically reviewed research on behavioral interventions based on the information-motivation-behavioral skills (IMB) model to investigate specific intervention strategies that focus on information, motivation, and behavioral skills and to evaluate their effectiveness for people with chronic diseases.MethodsA systematic review was conducted in accordance with the guidelines of both the National Evidence-based Healthcare Collaborating Agency and Im and Chang. A literature search was conducted using electronic databases. Randomized controlled trials that tested behavioral interventions based on the IMB model for promoting health behaviors among people with chronic diseases were included. Four investigators independently reviewed the studies and assessed the quality of each study. A narrative synthesis was used.ResultsA total of 12 studies were included in the review. Nine studies investigated patients with HIV/AIDS. The most frequently used intervention strategies were instructional pamphlets for the information construct, motivational interviewing techniques for the motivation construct, and instruction or role playing for the behavioral skills construct. Ten studies reported significant behavior changes at the first post-intervention assessment.ConclusionThis review indicates the potential strength of the IMB model as a theoretical framework to develop behavioral interventions. The specific integration strategies delineated for each construct of the model can be utilized to design model-based interventions

Validation of the Korean Version of the COVID-19 Phobia Scale (K-C19PS)

The purpose of this study was to evaluate the reliability and validity of a Korean version of the 20-item COVID-19 phobia tool, which was developed through a translation-reverse translation process. These data were collected from 226 persons using a self-reported questionnaire. Exploratory and confirmatory factor analyses were used to test construct validity. Finally, for 19 out of 20 items, the item-level convergence and differential validity were confirmed. In addition, the reliability and validity of the tool as a whole has been verified. For the subscales, Cronbach’s α was 0.90 for psychological, 0.87 for psychosomatic, 0.86 for economic, and 0.87 for social. Appropriate reliability was confirmed. Correlations between the COVID-19 phobia tool and fear of COVID-19 confirmed validity. The Korean version of the COVID-19 phobia tool is an appropriate scale for measuring the fear of COVID-19 and relevant psychological characteristics. Therefore, future studies in areas such as health and nursing could use this tool as required

Actin Filaments Play a Critical Role in Vacuolar Trafficking at the Golgi Complex in Plant Cells

Actin filaments are thought to play an important role in intracellular trafficking in various eukaryotic cells. However, their involvement in intracellular trafficking in plant cells has not been clearly demonstrated. Here, we investigated the roles actin filaments play in intracellular trafficking in plant cells using latrunculin B (Lat B), an inhibitor of actin filament assembly, or actin mutants that disrupt actin filaments when overexpressed. Lat B and actin2 mutant overexpression inhibited the trafficking of two vacuolar reporter proteins, sporamin:green fluorescent protein (GFP) and Arabidopsis thaliana aleurain-like protein:GFP, to the central vacuole; instead, a punctate staining pattern was observed. Colocalization experiments with various marker proteins indicated that these punctate stains corresponded to the Golgi complex. The A. thaliana vacuolar sorting receptor VSR-At, which mainly localizes to the prevacuolar compartment, also accumulated at the Golgi complex in the presence of Lat B. However, Lat B had no effect on the endoplasmic reticulum (ER) to Golgi trafficking of sialyltransferase or retrograde Golgi to ER trafficking. Lat B also failed to influence the Golgi to plasma membrane trafficking of H(+)-ATPase:GFP or the secretion of invertase:GFP. Based on these observations, we propose that actin filaments play a critical role in the trafficking of proteins from the Golgi complex to the central vacuole

Enhancement of Enteric Adenovirus Cultivation by Viral Transactivator Proteins▿

Human enteric adenoviruses (HAdVs; serotypes 40 and 41) are important waterborne and food-borne pathogens. However, HAdVs are fastidious, are difficult to cultivate, and do not produce a clear cytopathic effect during cell culture within a reasonable time. Thus, we examined whether the viral transactivator proteins cytomegalovirus (CMV) IE1 and hepatitis B virus (HBV) X promoted the multiplication of HAdVs. Additionally, we constructed a new 293 cell line expressing CMV IE1 protein for cultivation assays. We analyzed the nucleic acid sequences of the promoter regions of both E1A and hexon genes, which are considered to be the most important regions for HAdV replication. Expression of either HBV X or CMV IE1 protein significantly increased the promoter activities of E1A and hexon genes of HAdVs by as much as 14-fold during cell cultivation. The promotion of HAdV expression was confirmed by increased levels of both adenoviral DNA and mRNA expression. Finally, the newly developed 293 cell line expressing CMV IE1 protein showed an increase in viral DNA ranging from 574% to 619% compared with the conventional 293 cell line. These results suggest that the newly constructed cell line could be useful for efficient cultivation and research of fastidious HAdVs

Identification of the Protein Storage Vacuole and Protein Targeting to the Vacuole in Leaf Cells of Three Plant Species

Protein storage vacuoles (PSVs) are specialized vacuoles devoted to the accumulation of large amounts of protein in the storage tissues of plants. In this study, we investigated the presence of the storage vacuole and protein trafficking to the compartment in cells of tobacco (Nicotiana tabacum), common bean (Phaseolus vulgaris), and Arabidopsis leaf tissue. When we expressed phaseolin, the major storage protein of common bean, or an epitope-tagged version of α-tonoplast intrinsic protein (α-TIP, a tonoplast aquaporin of PSV), in protoplasts derived from leaf tissues, these proteins were targeted to a compartment ranging in size from 2 to 5 μm in all three plant species. Most Arabidopsis leaf cells have one of these organelles. In contrast, from one to five these organelles occurred in bean and tobacco leaf cells. Also, endogenous α-TIP is localized in a similar compartment in untransformed leaf cells of common bean and is colocalized with transiently expressed epitope-tagged α-TIP. In Arabidopsis, phaseolin contained N-glycans modified by Golgi enzymes and its traffic was sensitive to brefeldin A. However, trafficking of α-TIP was insensitive to brefeldin A treatment and was not affected by the dominant-negative mutant of AtRab1. In addition, a modified α-TIP with an insertion of an N-glycosylation site has the endoplasmic reticulum-type glycans. Finally, the early step of phaseolin traffic, from the endoplasmic reticulum to the Golgi complex, required the activity of the small GTPase Sar1p, a key component of coat protein complex II-coated vesicles, independent of the presence of the vacuolar sorting signal in phaseolin. Based on these results, we propose that the proteins we analyzed are targeted to the PSV or equivalent organelle in leaf cells and that proteins can be transported to the PSV by two different pathways, the Golgi-dependent and Golgi-independent pathways, depending on the individual cargo proteins

Intraoperative Hypotension Prediction Model Based on Systematic Feature Engineering and Machine Learning

Arterial hypotension is associated with incidence of postoperative complications, such as myocardial infarction or acute kidney injury. Little research has been conducted for the real-time prediction of hypotension, even though many studies have been performed to investigate the factors which affect hypotension events. This forecasting problem is quite challenging compared to diagnosis that detects high-risk patients at current. The forecasting problem that specifies when events occur is more challenging than the forecasting problem that does not specify the event time. In this work, we challenge the forecasting problem in 5 min advance. For that, we aim to build a systematic feature engineering method that is applicable regardless of vital sign species, as well as a machine learning model based on these features for real-time predictions 5 min before hypotension. The proposed feature extraction model includes statistical analysis, peak analysis, change analysis, and frequency analysis. After applying feature engineering on invasive blood pressure (IBP), we build a random forest model to differentiate a hypotension event from other normal samples. Our model yields an accuracy of 0.974, a precision of 0.904, and a recall of 0.511 for predicting hypotensive events

Comparison of Fatigue, Quality of Life, Turnover Intention, and Safety Incident Frequency between 2-Shift and 3-Shift Korean Nurses

This study aimed to compare the fatigue, quality of life, turnover intention, and safety incident frequency between 2- and 3-shift nurses, and analyze their perceptions of the 2-shift system. Participants were 227 nurses working for one year or more in a tertiary hospital in Seoul, South Korea (113 were 2-shift nurses for two months or longer, and 114 were 3-shift nurses with no experience of 2-shift work). The Occupational Fatigue Exhaustion Recovery Scale (OFER) and Quality of Life Scale were used. Turnover intention, safety incident frequency, and perceptions of the 2-shift system were surveyed by questionnaires developed by the researchers. Results showed that 2-shift nurses had lower chronic fatigue (t = −2.38, p = 0.018) and higher recovery between shifts (t = 3.90, p < 0.001) and quality of life scores than 3-shift nurses (t = 3.69, p < 0.001). There were no significant differences for turnover intention (t = −1.48, p = 0.140), frequency of needlestick accidents (t = 0.30, p = 0.763), medication errors (t = −1.46, p = 0.146), or near-miss medication errors (t = 0.78, p = 0.437). Two-shift nurses found it easier to secure rest and personal leisure time, and their shift system was shown to improve work satisfaction by increasing the continuity of care. Additional research is necessary to examine how nurses’ health status and emotional satisfaction vary by shift type

Detection and hazard assessment of pathogenic microorganisms in medical wastes

This study was undertaken to investigate the types and concentrations of microbial agents in various medical wastes as well as to characterize their survivals in these wastes at different temperatures for microbial risk assessment. Medical wastes collected from 5 major hospitals in South Korea were classified and stored at three different temperatures (-20, 6, and 30 degrees C). Presence of various microorganisms such as pathogenic viruses and bacteria were investigated by both cultivation and by (RT)-PCR assays. A number of (opportunistic) pathogenic bacteria, including Pseudomonas spp., Lactobacillus spp., Staphylococcus spp., Micrococcus spp., Kocuria spp., Brevibacillus spp., Microbacterium oxydans, and Propionibacterium acnes, were identified from the various medical wastes. In addition, pathogenic viruses such as noroviruses and hepatitis B virus were also detected in one of the human tissue wastes. Commonly identified bacterial and viral pathogens such as Pseudomonas spp., Corynebacterium diphtheriae, Escherichia coli, Staphylococcus spp., and respiratory synctial virus (RSV) were inoculated into either gauzes or diapers, and their survivals were characterized. Viral agents such as RSV showed poor survival in most environmental conditions, and demonstrated that various pathogens could be present in medical wastes but that the associated health risk appeared to be low. However, medical waste should be carefully controlled and monitored to prevent nosocomial infection associated with the exposure to these wastes.Lee J, 2008, APPL ENVIRON MICROB, V74, P2111, DOI 10.1128/AEM.02442-07Marinkovic N, 2008, WASTE MANAGE, V28, P1049, DOI 10.1016/j.wasman.2007.01.021Shariati B, 2007, J OCCUP HEALTH, V49, P317de Bruin E, 2006, J VIROL METHODS, V137, P259, DOI 10.1016/j.jviromet.2006.06.024Garcia C, 2006, J CLIN MICROBIOL, V44, P2997, DOI 10.1128/JCM.00065-06Jang YC, 2006, J ENVIRON MANAGE, V80, P107, DOI 10.1016/j.jenvman.2005.08.018ZIEBUHR W, 2006, INT J ANTIMICROB AG, V28, P14Phipps LP, 2004, J VIROL METHODS, V122, P119, DOI 10.1016/j/jviromet.2004.08.008Loberto JCS, 2004, BRAZ J MICROBIOL, V35, P64Seo SH, 2002, NAT MED, V8, P950, DOI 10.1038/nm757Nema SK, 2002, CURR SCI INDIA, V83, P271Katayama K, 2002, VIROLOGY, V299, P225, DOI 10.1006/viro.2002.1568Katayama H, 2002, APPL ENVIRON MICROB, V68, P1033, DOI 10.1128/AEM.68.3.1033-1039.2002Monpoeho S, 2001, APPL ENVIRON MICROB, V67, P2484SALKIN IF, 2001, REV HLTH IMPACTS MICManfredi R, 2000, EUR J EPIDEMIOL, V16, P111Kuo HW, 1999, WATER AIR SOIL POLL, V114, P413Kim BJ, 1999, J CLIN MICROBIOL, V37, P1714HAAS C, 1999, QUANTITATIVE MICROBIKane A, 1999, B WORLD HEALTH ORGAN, V77, P801PRUSS A, 1999, SAFE MANAGEMENT WAST, P20Schlegel L, 1998, EUR J CLIN MICROBIOL, V17, P887Frank U, 1997, CLIN INFECT DIS, V25, P318BELL DM, 1997, AM J MED S5B, V102, P9Lee CC, 1996, J HAZARD MATER, V48, P1GOLDENBERGER D, 1995, PCR METH APPL, V4, P368OHMAN SC, 1995, ACTA ODONTOL SCAND, V53, P49PATEL R, 1994, CLIN INFECT DIS, V18, P207PATTI JM, 1994, INFECT IMMUN, V62, P152TANAKA MM, 1994RUTALA WA, 1992, INFECT CONT HOSP EP, V13, P3848EDWARDS U, 1989, NUCLEIC ACIDS RES, V17, P7843