Interaction Effects between the 5-HTTLPR Genotype and Family Environments on Adolescent Alcohol Use and Misuse

Emerging evidence shows that the 5-HTTLPR genotype interacts with social environments and influences drinking behaviors. However, few studies have examined interaction effects between the 5-HTTLPR genotype and specific aspects of family environments on adolescent drinking. The present study investigated whether the effects of family conflict or parental monitoring on adolescent drinking differed as a function of the 5-HTTLPR genotype cross-sectionally and longitudinally. It was replicated in two independent samples consisting of 175 adolescents in the U.S. and 4,916 adolescents in the U.K. The results of path analyses and multi-group analyses showed that, in the two samples, the 5-HTTLPR low-activity allele carriers exposed to high levels of family conflict were more likely to engage in concurrent alcohol misuse at Time 1 than non-carriers. The results of analyses testing the interaction between the 5-HTTLPR genotype and parental monitoring were inconsistent in the two samples. Overall, our results suggest that the 5-HTTLPR low activity allele carriers are more susceptible to the effect of family conflict on concurrent alcohol misuse. This finding highlights the importance of identifying and reducing family conflict in alcohol prevention and intervention programs in adolescents with the 5-HTTLPR low-activity allele

Interaction Effects between the Cumulative Genetic Score and Psychosocial Stressor on Drinking Urge and Attentional Bias for Alcohol: A Human Laboratory Study

Stressful life events have been positively associated with alcohol use and misuse in young adults; however, individual differences in the association suggest the presence of moderators. Findings from observational studies suggest that the effects of stressful environments on drinking behavior may differ as a function of diverse single monoamine genes regulating serotonin and dopamine neurotransmission. However, research has not utilized an experimental design to examine whether the monoamine genes collectively are associated with the degree to which exposure to stressors affects alcohol endophenotypes. The current study examined whether the effects of an experimentally manipulated psychosocial stressor on drinking urge and attentional bias for alcohol cues differ as a function of the cumulative genetic index of 5-HTTLPR, MAO-A, DRD4, DAT1, and DRD2 genotypes (candidate genes and environment interaction; cGxE). The current study also examined whether salivary alpha-amylase level or anxiety state mediate the cGxE effects. One hundred five Caucasian young adults (mean age = 19.83; 61% male) went through both control and experimental stress conditions in order. Results showed that, as the cumulative genetic score of the five monoamine genes increased, attentional bias for alcohol-related stimuli elevated in the stress condition but not in the control condition. No mediating roles of salivary alpha-amylase and anxiety state in the cGxE effect were found, however. High cumulative genetic score of the five monoamine genes was associated with elevated drinking urge both in the control and stress conditions. Although replication is necessary, the findings suggest that the five monoamine genes collectively were positively associated with the cognitive process of an individual’s drive for alcohol (i.e., attentional bias) in stressful situations. The underlying psychological and neurobiological mechanisms need to be further characterized

Correlation between Galaxy Mergers and Luminous AGN

It is not yet clear what triggers the activity of active galactic nuclei (AGNs), but galaxy merging has been suspected to be one of the main mechanisms fuelling the activity. Using deep optical images taken at various ground-based telescopes, we investigate the fraction of galaxy mergers in 39 luminous AGNs (M$_{R}\, \lesssim$ -22.6 mag) at $z \leq$ 0.3 (a median redshift of 0.155), of which the host galaxies are generally considered as early-type galaxies. Through visual inspection of the images, we find that 17 of 39 AGN host galaxies (43.6%) show the evidence for current or past mergers like tidal tails, shells, and disturbed morphology. In order to see if this fraction is abnormally high, we also examined the merging fraction of normal early-type galaxies in the Sloan Digital Sky Survey (SDSS) Strip 82 data (a median redshift of 0.04), of which the surface-brightness limit is comparable to our imaging data. To correct for the effects related to the redshift difference of the two samples, we performed an image simulation by putting a bright point source as an artificial AGN in the images of SDSS early-type galaxies and placing them onto the redshifts of AGNs. The merging fraction in this realistic sample of simulated AGNs is only $\sim 5 - 15\%$ ($1/4$ to $1/8$ of that of real AGNs). Our result strongly suggests that luminous AGN activity is associated with galaxy merging.Comment: 57 pages, 19 figures, published in Astrophysical Journa

Mycotoxin production of Alternaria strains isolated from Korean barley grains determined by LC-MS/MS

Twenty-four Alternaria strains were isolated from barley grain samples. These strains were screened for the production of mycotoxins on rice medium using thin layer chromatography. All 24 strains produced at least one of the five mycotoxins (ALT, AOH, ATX-I, AME, and TeA). Three representative strains, namely EML-BLDF1-4, EML-BLDF1-14, and EML-BLDF1-18, were further analyzed using a new LC–MS/MS-based mycotoxin quantification method. This method was used to detect and quantify Alternaria mycotoxins. We used positive ion electrospray mass spectrometry with multiple reaction mode (MRM) for the simultaneous quantification of various Alternaria mycotoxins produced by these strains. Five Alternaria toxins (ALT, ATX-I, AOH, AME, and TeA) were detected and quantified. Sample preparation included methanol extraction, concentration, and injection into LC–MS/MS. Limit of detection ranged from 0.13 to 4 μg/mL and limit of quantification ranged from 0.25 to 8 μg/mL

Ecstasy use and suicidal behavior among adolescents: findings from a national survey

The relationship between ecstasy use and suicidal behavior among adolescents in the United States was examined. Data from the adolescent subsample (ages 12-17, N = 19,301) of the 2000 National Household Survey on Drug Abuse were used in the analyses. Information on adolescent substance use, suicidal behaviors, and related sociodemographic, family, and individual factors was obtained in the survey. The rate of past year suicide attempt among adolescents with lifetime ecstasy use was almost double that of adolescents who had used other drugs only, and nine times that of adolescents with no history of illicit drug use. In multinomial logistic regression analyses controlling for related factors, the effect of ecstasy use remained significant. Adolescent ecstasy users may require enhanced suicide prevention and intervention efforts

Discovery of a Supercluster at z ~ 0.91 and Testing the ΛCDM Cosmological Model

The ΛCDM cosmological model successfully reproduces many aspects of the galaxy and structure formation of the universe. However, the growth of large-scale structures (LSSs) in the early universe is not well tested yet with observational data. Here, we have utilized wide and deep optical–near-infrared data in order to search for distant galaxy clusters and superclusters (0.8 < z < 1.2). From the spectroscopic observation with the Inamori Magellan Areal Camera and Spectrograph (IMACS) on the Magellan telescope, three massive clusters at z ~ 0.91 are confirmed in the SSA22 field. Interestingly, all of them have similar redshifts within Δ z ~ 0.01 with velocity dispersions ranging from 470 to 1300 km s−1. Moreover, as the maximum separation is ~15 Mpc, they compose a supercluster at z ~ 0.91, meaning that this is one of the most massive superclusters at this redshift to date. The galaxy density map implies that the confirmed clusters are embedded in a larger structure stretching over ~100 Mpc. ΛCDM models predict about one supercluster like this in our surveyed volume, consistent with our finding so far. However, there are more supercluster candidates in this field, suggesting that additional studies are required to determine if the ΛCDM cosmological model can successfully reproduce the LSSs at high redshift

Dissolution behaviors of PuO2(cr) in natural waters

PuO2(cr) dissolution in natural water was investigated at 25°C and 60°C under atmospheric conditions. The concentration of Pu in solutions [Pu], was monitored for 1 year of reaction time. PuO2(cr) dissolution in natural water reached a steady state within 2 months at 25°C. The [Pu] in groundwater and seawater at pH 8 were in the range of [Pu] = 0.9–34 and 3.4–27 nM, respectively. The [Pu] in concrete porewater (rainwater equilibrated with concrete) at pH 8.1–10.9 was in the range of 0.1–3.2 nM. The [Pu] and pH values of groundwater were similar to those of seawater samples having a high ionic strength. The measured [Pu] at equilibrium in all samples was higher than the calculated solubility curves for PuO2(am, hyd). Experimental evidence is insufficient to confirm the oxidation state of Pu in solution and solid phases. However, the results of geochemical modeling indicate that PuO2(am, hyd) and aqueous Pu(IV) species are dominant in natural water samples of this work. The dissolution behavior of PuO2(cr) in natural waters is comparable to the oxidative dissolution of PuO2(am, hyd) in the presence of PuO2(coll, hyd). The dissolution of PuO2 in groundwater decreased at higher temperatures, whereas the influence of temperature in seawater and porewater was not significant under these experimental conditions

Syk/Src Pathway-Targeted Inhibition of Skin Inflammatory Responses by Carnosic Acid

Carnosic acid (CA) is a diterpene compound exhibiting antioxidative, anticancer, anti-angiogenic, anti-inflammatory, anti-metabolic disorder, and hepatoprotective and neuroprotective activities. In this study, the effect of CA on various skin inflammatory responses and its inhibitory mechanism were examined. CA strongly suppressed the production of IL-6, IL-8, and MCP-1 from keratinocyte HaCaT cells stimulated with sodium lauryl sulfate (SLS) and retinoic acid (RA). In addition, CA blocked the release of nitric oxide (NO), tumor necrosis factor (TNF)-α, and prostaglandin E2 (PGE2) from RAW264.7 cells activated by the toll-like receptor (TLR)-2 ligands, Gram-positive bacterium-derived peptidoglycan (PGN) and pam3CSK, and the TLR4 ligand, Gram-negative bacterium-derived lipopolysaccharide (LPS). CA arrested the growth of dermatitis-inducing Gram-positive and Gram-negative microorganisms such Propionibacterium acnes, Pseudomonas aeruginosa, and Staphylococcus aureus. CA also blocked the nuclear translocation of nuclear factor (NF)-κB and its upstream signaling including Syk/Src, phosphoinositide 3-kinase (PI3K), Akt, inhibitor of κBα (IκBα) kinase (IKK), and IκBα for NF-κB activation. Kinase assays revealed that Syk could be direct enzymatic target of CA in its anti-inflammatory action. Therefore, our data strongly suggest the potential of CA as an anti-inflammatory drug against skin inflammatory responses with Src/NF-κB inhibitory properties