4 research outputs found
Live imaging of mRNA using RNA-stabilized fluorogenic proteins
Fluorogenic RNA aptamers bind and activate the fluorescence of otherwise nonfluorescent dyes. However, fluorogenic aptamers are limited by the small number of fluorogenic dyes suitable for use in live cells. In this communication, fluorogenic proteins whose fluorescence is activated by RNA aptamers are described. Fluorogenic proteins are highly unstable until they bind RNA aptamers inserted into messenger RNAs, resulting in fluorescent RNA–protein complexes that enable live imaging of mRNA in living cells
Liposome-Based Engineering of Cells To Package Hydrophobic Compounds in Membrane Vesicles for Tumor Penetration
Natural membrane vesicles (MVs) derived
from various types of cells play an essential role in transporting
biological materials between cells. Here, we show that exogenous compounds
are packaged in the MVs by engineering the parental cells via liposomes,
and the MVs mediate autonomous intercellular migration of the compounds
through multiple cancer cell layers. Hydrophobic compounds delivered
selectively to the plasma membrane of cancer cells using synthetic
membrane fusogenic liposomes were efficiently incorporated into the
membrane of MVs secreted from the cells and then transferred to neighboring
cells via the MVs. This liposome-mediated MV engineering strategy
allowed hydrophobic photosensitizers to significantly penetrate both
spheroids and in vivo tumors, thereby enhancing the therapeutic efficacy.
These results suggest that innate biological transport systems can
be in situ engineered via synthetic liposomes to guide the penetration
of chemotherapeutics across challenging tissue barriers in solid tumors
Live imaging of mRNA using RNA-stabilized fluorogenic proteins
Fluorogenic RNA aptamers bind and activate the fluorescence of otherwise nonfluorescent dyes. However, fluorogenic aptamers are limited by the small number of fluorogenic dyes suitable for use in live cells. In this communication, fluorogenic proteins whose fluorescence is activated by RNA aptamers are described. Fluorogenic proteins are highly unstable until they bind RNA aptamers inserted into messenger RNAs, resulting in fluorescent RNA–protein complexes that enable live imaging of mRNA in living cells