A High-Precision Micropipette Sensor for Cellular-Level Real-Time Thermal Characterization

We report herein development of a novel glass micropipette thermal sensor fabricated in a cost-effective manner, which is capable of measuring steady thermal fluctuation at spatial resolution of similar to 2 mu m with an accuracy of +/- 0.01 degrees C. We produced and tested various micrometer-sized sensors, ranging from 2 mu m to 30 mu m. The sensor comprises unleaded low-melting-point solder alloy (Sn-based) as a core metal inside a pulled borosilicate glass pipette and a thin film of nickel coating outside, creating a thermocouple junction at the tip. The sensor was calibrated using a thermally insulated calibration chamber, the temperature of which can be controlled with an accuracy of +/- 0.01 degrees C, and the thermoelectric power (Seebeck coefficient) of the sensor was recorded from 8.46 to 8.86 mu V/degrees C. We have demonstrated the capability of measuring temperatures at a cellular level by inserting our temperature sensor into the membrane of a live retinal pigment epithelium cell subjected to a laser beam with a focal spot of 6 mu m. We measured transient temperature profiles and the maximum temperatures were in the range of 38-55 +/- 0.5 degrees C.open111212sciescopu

Characteristics of vertical velocities estimated from drop size and fall velocity spectra of a Parsivel disdrometer

Vertical air velocities were estimated from drop size and fall velocity spectra observed by Parsivel disdrometers during intensive field observations from 13 June to 3 August 2016 around Mt. Jiri (1915ma.s.l.) in the southern Korean Peninsula. Rainfall and wind velocity data measured by Parsivel disdrometers and ultrasonic anemometers, respectively, were analyzed for an orographic rainfall event associated with a stationary front over Mt. Jiri on 1 July 2016. In this study, a new technique was developed to estimate vertical air velocities from drop size and fall velocity spectra measured by the Parsivel disdrometers and investigate characteristics of up-/downdrafts and related microphysics on the windward and leeward sides of the mountain. To validate results from this technique, vertical air velocities between the Parsivel disdrometers and anemometers were compared at different locations and were shown in quite good agreement with each other. It was shown that upward motion was relatively more dominant on the windward side and even during periods of heavy rainfall. In contrast, downward motion was more dominant on the leeward side during nearly the same periods of heavy rainfall. Occurrences of upward and downward motion were digitized as percentage values as they are divided by a total count of occurrences during the entire period. On the windward (leeward) side, the percentages of upward (downward) motion were much larger than those of downward (upward) motion. The mean rainfall intensity on the leeward side was stronger than on the windward side, suggesting that most of the rainfall on the leeward side was relatively more affected by the downward motion. With the estimated vertical air velocities, histogram characteristics of rainfall parameters were also examined between the windward and leeward sides

Labisia pumila extract protects skin cells from photoaging caused by UVB irradiation

Labisia pumila (Myrsinaceae), known as "Kacip Fatimah," has been used by many generations of Malay women to induce and facilitate child birth as well as a post partum medicine. However, its topical application on skin has not been reported yet. In this study, we have focused on the anti-photoaging effects of L. pumila. Extract of L. pumila was first analyzed for their antioxidant activities using DPPH (2,2-diphenyl-1-picrylhydrazyl) since UV irradiation is a primary cause of reactive oxygen species (ROS) generation in the skin. The 50% free radical scavenging activity (FSC(50)) of L. pumila extract was determined to be 0.006%, which was equal to that produced by 156 microM ascorbic acid. TNF-alpha and cyclooxygenase (COX-2) play a primary role in the inflammation process upon UV irradiation and are known to be stimulated by UVB. Treatment with L. pumila extract markedly inhibited the TNF-alpha production and the expression of COX-2. Decreased collagen synthesis of human fibroblasts by UVB was restored back to normal level after treatment with L. pumila extract. On the other hand, the enhanced MMP-1 expression upon UVB irradiation was down regulated by L. pumila extract in a dose-dependent manner. Furthermore, treatment of normal keratinocytes with L. pumila extract attenuated UVB-induced MMP-9 expression. These results collectively suggest L. pumila extract has tremendous potential as an anti-photoaging cosmetic ingredient

A Transformation-Based Learning Method on Generating Korean Standard Pronunciation

PACLIC 21 / Seoul National University, Seoul, Korea / November 1-3, 200