2,763 research outputs found
Growth, detection, quantification, and inactivation of SARS-CoV-2
Severe acute respiratory syndrome coronavirus (SARS-CoV)-2 is the agent responsible for the coronavirus disease 2019 (COVID-19) global pandemic. SARS-CoV-2 is closely related to SARS-CoV, which caused the 2003 SARS outbreak. Although numerous reagents were developed to study SARS-CoV infections, few have been applicable to evaluating SARS-CoV-2 infection and immunity. Current limitations in studying SARS-CoV-2 include few validated assays with fully replication-competent wild-type virus. We have developed protocols to propagate, quantify, and work with infectious SARS-CoV-2. Here, we describe: (1) virus stock generation, (2) RT-qPCR quantification of SARS-CoV-2 RNA; (3) detection of SARS-CoV-2 antigen by flow cytometry, (4) quantification of infectious SARS-CoV-2 by focus-forming and plaque assays; and (5) validated protocols for virus inactivation. Collectively, these methods can be adapted to a variety of experimental designs, which should accelerate our understanding of SARS-CoV-2 biology and the development of effective countermeasures against COVID-19
Older, Less Regulated Medical Marijuana Programs Have Much Greater Enrollment Rates Than Newer âMedicalizedâ Programs
Twenty-three states and the District of Columbia have passed laws implementing medical marijuana programs. The nineteen programs that were in operation as of October 2014 collectively had over one million participants. All states (including D.C.) with medical marijuana laws require physicians directly or indirectly to authorize the use of marijuana at their discretion, yet little is known about how medical marijuana programs vary regarding adherence to basic principles of medical practice and associated rates of enrollment. To explore this, we analyzed marijuana programs according to seven components of traditional medical care and pharmaceutical regulation. We then examined enrollment rates, while controlling for potentially confounding state characteristics. We found that fourteen of the twenty-four programs were nonmedical and collectively enrolled 99.4 percent of participants nationwide, with enrollment rates twenty times greater than programs deemed to be âmedicalized.â Policy makers implementing or amending medical marijuana programs should consider the powerful relationship between less regulation and greater enrollment. Researchers should consider variations across programs when assessing programsâ population-level effects
ELVIS: Entertainment-led video summaries
© ACM, 2010. This is the author's version of the work. It is posted here by permission of ACM for your personal use. Not for redistribution. The definitive version was published in ACM Transactions on Multimedia Computing, Communications, and Applications, 6(3): Article no. 17 (2010) http://doi.acm.org/10.1145/1823746.1823751Video summaries present the user with a condensed and succinct representation of the content of a video stream. Usually this is achieved by attaching degrees of importance to low-level image, audio and text features. However, video content elicits strong and measurable physiological responses in the user, which are potentially rich indicators of what video content is memorable to or emotionally engaging for an individual user. This article proposes a technique that exploits such physiological responses to a given video stream by a given user to produce Entertainment-Led VIdeo Summaries (ELVIS). ELVIS is made up of five analysis phases which correspond to the analyses of five physiological response measures: electro-dermal response (EDR), heart rate (HR), blood volume pulse (BVP), respiration rate (RR), and respiration amplitude (RA). Through these analyses, the temporal locations of the most entertaining video subsegments, as they occur within the video stream as a whole, are automatically identified. The effectiveness of the ELVIS technique is verified through a statistical analysis of data collected during a set of user trials. Our results show that ELVIS is more consistent than RANDOM, EDR, HR, BVP, RR and RA selections in identifying the most entertaining video subsegments for content in the comedy, horror/comedy, and horror genres. Subjective user reports also reveal that ELVIS video summaries are comparatively easy to understand, enjoyable, and informative
The Deuterium to Hydrogen Abundance Ratio Towards a Fourth QSO: HS0105+1619
We report the measurement of the primordial D/H abundance ratio towards QSO
\object. The column density of the hydrogen in the Lyman limit
system is high, \lnhi \cmm, allowing for the deuterium to
be seen in 5 Lyman series transitions. The measured value of the D/H ratio
towards QSO \object is found to be D/H. The
metallicity of the system showing D/H is found to be solar,
indicating that the measured D/H is the primordial D/H within the measurement
errors. The gas which shows D/H is neutral, unlike previous D/H systems which
were more highly ionized. Thus, the determination of the D/H ratio becomes more
secure since we are measuring it in different astrophysical environments, but
the error is larger because we now see more dispersion between measurements.
Combined with prior measurements of D/H, the best D/H ratio is now D/H, which is 10% lower than the previous value. The new
values for the baryon to photon ratio, and baryonic matter density derived from
D/H are and \ob
respectively.Comment: Minor text and reference changes. To appear in the May 10, 2001 issue
of the Astrophysical Journa
Asymptotics and local constancy of characters of p-adic groups
In this paper we study quantitative aspects of trace characters
of reductive -adic groups when the representation varies. Our approach
is based on the local constancy of characters and we survey some other related
results. We formulate a conjecture on the behavior of relative to
the formal degree of , which we are able to prove in the case where
is a tame supercuspidal. The proof builds on J.-K.~Yu's construction and the
structure of Moy-Prasad subgroups.Comment: Proceedings of Simons symposium on the trace formul
Structure of Venezuelan equine encephalitis virus in complex with the LDLRAD3 receptor
LDLRAD3 is a recently defined attachment and entry receptor for Venezuelan equine encephalitis virus (VEEV
Gravitating Model Solitons
We study axially symmetric static solitons of O(3) nonlinear model
coupled to (2+1)-dimensional anti-de Sitter gravity. The obtained solutions are
not self-dual under static metric. The usual regular topological lump solution
cannot form a black hole even though the scale of symmetry breaking is
increased. There exist nontopological solitons of half integral winding in a
given model, and the corresponding spacetimes involve charged Baados-Teitelboim-Zanelli black holes without non-Abelian scalar hair.Comment: 35 pages, RevTe
- âŠ