The effects of Stanozolol and high protein diet on Colon Anastomosis

Background: Anastomotic leakage after colonic resection and anastomosis are serious complications with high morbidity and mortality. Collagen synthesis is an essential feature of anastomotic healing. Objective: Our study aimed to investigate individual and combined effects of high protein diet and Stanozolol on the intestinal anastomosis in rats. Methods: Total of 48 Wistar-albino rats were divided into four groups. Group C: Control group; Group P: high protein diet (4mg/day) administered by gavage; Group A: group to which stanozolol was administered intramuscularly (i.m) (2mg/kg/day); Group A+P: group to which both stanozolol (2mg/kg/day) i.m and high protein diet (4mg/day by gavage) was administered. In each group, half of the animals underwent surgery on the 3rd postoperative (PO) day and the other half on the 7th PO day for analysis procedures. Bursting pressures of each anastomotic segment, tissue hydroxyproline levels, and histologic tissue repair process were evaluated. Results: On 7th PO day, group A+P had statistically and significantly higher bursting pressures than the control group. Hydroxyproline levels at the anastomotic segment on both 3rd and 7th PO days were also significantly higher in group A+P. Conclusion: A high protein diet and stanozolol do not significantly impact the anastomotic healing when used separately. We concluded that high protein diet and Stanozolol can be used after colorectal surgery in humans but requires further research

Prognostic effects of SuPAR and Neopterin Levels on Patients with Lung Cancer

karahan, irfan/0000-0003-4669-1751WOS:000547384800001Background: Two unique biomarkers, soluble form of the urokinase-type plasminogen activator receptor (suPAR) and neopterin, play a crucial role in inflammatory processes. This study aimed to reveal whether it is possible to utilize these biomarkers in predicting tumor prognosis in patients with lung cancers. Methods: The present study was designed as a single center, prospective, and controlled research. The study was conducted with forty patients with lung cancer (case group) and 41 healthy individuals (control group) in Kirikkale University, Faculty of Medicine between 2016-2020. The case group was also divided into two of the early and advanced stages. The blood samples were drawn to evaluate suPAR and neopterin levels, and these parameters were compared between the case and control groups. Also, the prognostic effects of age, stage of the tumor, and the levels of mentioned parameters were investigated with the survival analysis. Results: The median duration of the follow-up was 32 (4-75) months. suPAR and neopterin levels were found to be higher in the case group than in the control group. Cox regression showed that the high levels of neopterin and suPAR increased mortality risk [1)=0.002, HR: 1.25 (1.081.45 95%CI) and p=0.023, HR:1.07 (1.01-1.13), respectively]. Finally, age and stage of the tumor were found to have no relationship with survival. Conclusion: suPAR and neopterin as members of the inflammatory pathway were found to be higher in cancer cases. Furthermore, both suPAR and neopterin levels were found to be predictive for the mortality of patients with lung cancers; therefore, they are thought to be used for the management of cancer

Effect of Naloxone on Oxidative Stress and Testicular Injury due to Spermatic Vessel Ligation of Rat Testis

altug, mustafa ugur/0000-0002-6475-2178; Aydos, Tolga Resat/0000-0002-1832-9336WOS: 000260236500007PubMed: 18931543Aims: Two-stage Fowler-Stephens orchiopexy has been accompanied by testicular atrophy in some cases but neither of the mechanisms responsible for testicular injury are clear, nor is there an effective agent that might prevent this injury. In this study we aimed to investigate the long-term effects of naloxone, a morphine antagonist, on testicular histopathology and oxidative stress after spermatic vessel ligation (SVL) in rats. Methods: 32 prepubertal rats were randomly divided into four equal groups: group 1: control (only bilateral orchiectomies were performed); group 2: sham-operated group; group 3: SVL, and group 4: SVL+naloxone (1 mg/kg twice daily for 1 month). One month postoperatively, bilateral orchiectomies were performed to evaluate histopathologic findings and measurement of malondialdehyde (MDA) and nitric oxide (NO) levels. Results: Considering group 3, left SVL resulted in significant tissue damage in both testes, more severe in the ipsilateral testis. The SVL resulted in a significant increase in testicular MDA levels of both testes in this group (p 0.05), the contralateral testicular histopathology improved significantly (p < 0.05). However, naloxone did not change either testicular MDA or NO levels. Conclusions: The SVL led to bilateral testicular injury, and oxidative stress may be a reason for this injury. Naloxone significantly improved contralateral testicular injury without showing any antioxidative effect. Copyright (c) 2008 S. Karger AG, Base