Question on a Bus

May I - , George cleared his throat. I wonder if I could take Jean to the show with me tonight. His words were engulfed by the silence that spread over the table. (Why doesn\u27t somebody say something\u27? They can\u27t say no, they just can\u27t. What are they waiting for?) I wouldn\u27t be in late and there is no school tomorrow. The mother looked up and smiled, and looked to the father for the first word. He said, Jean who? Jean Newcomb. She is in my English class. (And she smiles when she looks at me, and when she smiles I want to smile too. But I can\u27t say that.) She lives on the south side. Is she a nice girl? Sure, Mom, she\u27s swell! The mother looked down at her plate. That is, she\u27s alright. She\u27s a nice girl

Microbiological methods for the water recovery systems test, revision 1.1

Current microbiological parameters specified to verify microbiological quality of Space Station Freedom water quality include the enumeration of total bacteria, anaerobes, aerobes, yeasts and molds, enteric bacteria, gram positives, gram negatives, and E. coli. In addition, other parameters have been identified as necessary to support the Water Recovery Test activities to be conducted at the NASA/MSFC later this year. These other parameters include aerotolerant eutrophic mesophiles, legionellae, and an additional method for heterotrophic bacteria. If inter-laboratory data are to be compared to evaluate quality, analytical methods must be eliminated as a variable. Therefore, each participating laboratory must utilize the same analytical methods and procedures. Without this standardization, data can be neither compared nor validated between laboratories. Multiple laboratory participation represents a conservative approach to insure quality and completeness of data. Invariably, sample loss will occur in transport and analyses. Natural variance is a reality on any test of this magnitude and is further enhanced because biological entities, capable of growth and death, are specific parameters of interest. The large variation due to the participation of human test subjects has been noted with previous testing. The resultant data might be dismissed as 'out of control' unless intra-laboratory control is included as part of the method or if participating laboratories are not available for verification. The purpose of this document is to provide standardized laboratory procedures for the enumeration of certain microorganisms in water and wastewater specific to the water recovery systems test. The document consists of ten separate cultural methods and one direct count procedure. It is not intended nor is it implied to be a complete microbiological methods manual