Evaluation of Technical Efficiency of Rabbit Production in Buuri Sub -County, Meru County, Kenya

Current rabbit production in Buuri Sub county stands at 1.2 Metric tons of meat against a potential of 8.4 Metric tons per year. This productivity gap is wide and indicative of poor and low performance of the enterprise in rural areas of Kenya and specifically Buuri Sub County. Thus the main objective of this study was to investigate the technical efficiency of rabbit production and its contribution to household food production and family welfare under conditions of resource scarcity. The technical efficiency of rabbit production was evaluated to explain the paradox behind the low productivity of rabbit enterprises in Buuri sub-county. A multistage simple random sampling procedure was employed to get 139 respondents for the study. A semi structured and pre-tested questionnaire was used to collect data from the selected small holder rabbit producers through face to face interview of the household heads. The study used descriptive statistics for the analysis of socioeconomic and institutional attributes of the rabbit producers. The stochastic frontier production parametric method was used for the efficiency analysis. The results showed mean technical efficiencies among the rabbit farms were 36.83%. The farmers are not producing the rabbit output at minimum costs.  Further the study found that the capital is the most important rabbit output enhancing variable among all studied parameters. The Tobit model results indicated that increased access to education, trainings and credit to the farmers led to improved rabbit efficiency. More importantly county government and non-governmental agencies should make deliberate attempts for improved farmer-extension and research linkage for better technology transfer and adoption by farmers, leading to more productive rabbit enterprises hence improved incomes and thus reducing poverty level among farmers. Keywords: Technical efficiency, rabbit breeds, efficiency, rabbit keeping, backyard farming enterprises, stochastic frontie

Unlocking the potential of substrate quality for the enhanced antibacterial activity of black soldier fly against pathogens

SUPPORTING INFORMATION : Pearson’s correlation coefficient (r) for proximate analysis and antibacterial activity of hexane extract (Figure S1); concentrations of methylated fatty acid from GC-MS (Table S1); mass of HIL in different growth stages (Table S2); antibacterial activity of HIL extracted with hexane (Table S3); antibacterial activity of HIL extracted with 20% acetic acid (Table S4); and antibacterial activity of HIL extracted with 80% methanol (Table S5).Globally, antibiotics are facing fierce resistance from multidrug-resistant bacterial strains. There is an urgent need for eco-friendly alternatives. Though insects are important targets for antimicrobial peptides, it has received limited research attention. This study investigated the impact of waste substrates on the production of antibacterial agents in black soldier fly (Hermetia illucens L.) larvae (HIL) and their implications in the suppression of pathogens [Bacillus subtilis (ATCC 6051), Staphylococcus aureus (ATCC 25923), Pseudomonas aeruginosa (ATCC 27853), and Escherichia coli (ATCC 25922)]. The 20% acetic acid (AcOH) extract from market waste had the highest antibacterial activity with an inhibition zone of 17.00 mm, followed by potato waste (15.02 mm) against S. aureus. Hexane extract from HIL raised on market waste also showed a significant inhibitory zone (13.06 mm) against B. subtilis. Minimum inhibitory concentration (MIC) values recorded were 25 mg/mL against all test pathogens. The fastest time-kill of 20% AcOH extract was 4 h against B. subtilis, E. coli, andP. aeruginosa. Lauric acid was also identified as the dominant component of the various hexane extracts with concentrations of 602.76 and 318.17 μg/g in HIL reared on potato and market waste, respectively. Energy from the market waste substrate correlated significantly (r = 0.97) with antibacterial activities. This study highlights the key role of substrate quality and extraction methods for enhancing the production of antibacterial agents in HIL, thus providing new insights into the development of potential drugs to overcome the alarming concerns of antimicrobial resistance.Australian Centre for International Agricultural Research, Norwegian Agency for Development Cooperation, Norad, Novo Nordisk Foundation, the Rockefeller Foundation, Bill & Melinda Gates Foundation, Horizon Europe, the Curt Bergfors Foundation Food Planet Prize Award, Norwegian Agency for Development Cooperation, the Section for Research, Innovation, and Higher Education, Egerton University and icipe core funding provided by the Swedish International Development Cooperation Agency (Sida); the Swiss Agency for Development and Cooperation (SDC); the Australian Centre for International Agricultural Research (ACIAR); the Norwegian Agency for Development Cooperation (Norad); the Federal Democratic Republic of Ethiopia; and the Government of the Republic of Kenya.https://pubs.acs.org/journal/acsodfhj2024ChemistrySDG-03:Good heatlh and well-bein

Unearthing Lactococcus lactis and Scheffersomyeces symbionts from edible wood-boring beetle larvae as a bio-resource for industrial applications

Abstract Background Gut microbiota have several advantages in influencing the host nutrition, metabolism, immunity and growth. However, the understanding of the gut microbiota in key edible wood-boring beetle larvae remain largely undefined. In the present study, the characteristics of the gut microbiota of two edible wood-boring species (Titocerus jaspideus and Passalus punctiger) from two indigenous forested areas were investigated. Results Over 50% of Amplicon Sequence Variants (ASVs) constituted of Firmicutes in T. jaspideus. The dominant phyla in both beetle species were Bacteroidota (4.20–19.79%) and Proteobacteria (15.10–23.90%). Lactococcus lactis was the most abundant and core prokaryote in the guts of T. jaspideus. The fungi identified in the gut of both insects belong to the phylum Obazoa (66%) and Ascomycota (> 15%). Scheffersomyeces sp. was the core eukaryote recorded. The diversity of gut microbiota in both insect species did not vary significantly. Most of the prokaryotic genes expressed were predominantly associated with biosynthesis and metabolism. Conclusion Our findings demonstrated that Lactococcus lactis and Scheffersomyeces are core gut microbes of wood boring beetle larvae with desirable probiotic properties and promising use in food product fermentation for improved growth performance, gut barrier health, intestinal flora balance and immune protection for human and animals. Further studies to highlight the latest medical-based applications of L. lactis as live-delivery vector for the administration of therapeutics against both communicable and non-communicable diseases are warranted

Unlocking the Potential of Substrate Quality for the Enhanced Antibacterial Activity of Black Soldier Fly against Pathogens

Globally, antibiotics are facing fierce resistance from multidrug-resistant bacterial strains. There is an urgent need for eco-friendly alternatives. Though insects are important targets for antimicrobial peptides, it has received limited research attention. This study investigated the impact of waste substrates on the production of antibacterial agents in black soldier fly (Hermetia illucens L.) larvae (HIL) and their implications in the suppression of pathogens [Bacillus subtilis (ATCC 6051), Staphylococcus aureus (ATCC 25923), Pseudomonas aeruginosa (ATCC 27853), and Escherichia coli (ATCC 25922)]. The 20% acetic acid (AcOH) extract from market waste had the highest antibacterial activity with an inhibition zone of 17.00 mm, followed by potato waste (15.02 mm) against S. aureus. Hexane extract from HIL raised on market waste also showed a significant inhibitory zone (13.06 mm) against B. subtilis. .Minimum inhibitory concentration (MIC) values recorded were 25 mg/mL against all test pathogens. The fastest time-kill of 20% AcOH extract was 4 h againstB. subtilis, E. coli, ,andP. aeruginosa. Lauric acid was also identified as the dominant component of the various hexane extracts with concentrations of 602.76 and 318.17 μg/g in HIL reared on potato and market waste, respectively. Energy from the market waste substrate correlated significantly (r = 0.97) with antibacterial activities. This study highlights the key role of substrate quality and extraction methods for enhancing the production of antibacterial agents in HIL, thus providing new insights into the development of potential drugs to overcome the alarming concerns of antimicrobial resistance