The complete chloroplast genome sequence of Campanula zangezura (Campanulaceae)

The complete chloroplast genome sequence of Campanula zangezura was determined by Illumina pair-end sequencing. The complete cp genome was 166,331 bp in length, containing a large single-copy (LSC) region of 100,034 bp and a small single-copy (SSC) region of 7781 bp, which were separated by a pair of 29,258 bp inverted repeats (IRs). A total of 113 unique genes were annotated, including 79 protein-coding genes, 30 tRNA genes, and 4 rRNA genes. Among these genes, 17 genes contained one or two introns. The ML tree based on 74 protein-coding genes showed that C. zangezura formed a sister to the Campanula punctata and Campanula takesimana clade

The complete plastid genome sequence of Viola selkirkii Pursh ex Goldie (Violaceae)

Viola selkirkii, belonging to the genus Viola, has heart-shaped leaves and pale purple flowers, and it is widely distributed in the Northern Hemisphere, including Europe, North America, and Asia. In this study, the plastid genome of V. selkirkii was sequenced and phylogenetic analysis was performed on 11 Viola plastid genomes. The length of the plastid genome length of V. selkirkii was 156,774 bp, and it was identified as having a typical quadripartite structure with a large single-copy region (85,930 bp), a small single-copy region (17,982 bp), and two inverted repeat regions (26,431 bp each). A phylogenetic analysis was conducted with 77 protein-coding genes from the complete plastid genomes of 11 Viola and nine Salicaceae species; the complete plastid genome of Erythroxylum novogranatense was used as an outgroup. Viola formed a monophyletic clade, and V. selkirkii was closely related to V. ulleungdoensis. These results contribute to the clear identification of the phylogenetic position of V. selkirkii in Viola

The complete plastid genome sequence of Chloranthus fortunei (A. Gray) Solms-Laub. in Chloranthaceae

Chloranthus fortunei (A. Gray) Solms-Laub. is a perennial herb in a basal angiosperm family Chloranthaceae. Here, we reported the complete plastid genome of C. fortunei using Illumina short-read data. The total genome size was 157,063 bp in length, containing 79 protein-coding genes, 30 tRNA genes, and four rRNA genes. The gene content and order were consistent with previously reported Chloranthus plastid genomes. The overall GC content of the C. fortunei plastid genome was 39.0%. In the phylogenetic result, genus Chloranthus was monophyletic and divided into two subclades: C. japonicus+C. angustifolius+C. fortunei, and C. henryi+C. spicatus+C. erectus. Our phylogenetic result was consistent with previous phylogenetic studies, and was supported by a previously proposed infrageneric classification of the genus Chloranthus

The complete chloroplast genome sequence of Viola japonica (Violaceae)

We determined the complete chloroplast genome sequence of Viola japonica using Illumina pair-end sequencing data. The whole chloroplast genome was 156,504 bp in length, consisting of a pair of inverted repeats (IR) of 26,427 bp, a large single-copy (LSC) region of 85,647 bp, and a small single-copy (SSC) region of 18,003 bp. The gene contents and order were exactly the same as published five Violaceae plastomes. A total of 111 unique genes were annotated, including 77 protein-coding genes, 30 tRNAs, and 4 rRNAs. Among the annotated genes, 16 genes contained one or two introns. The ML tree showed that V. japonica formed a clade with Viola seoulensis

Phylogenetic tree reconstruction based on 76 protein coding genes using the ML.

<p>Bootstrap values are shown below the clades, and Bayesian posterior probabilities are shown above the clades.</p

IR contraction in the <i>Adenophora stricta</i> chloroplast genome.

<p>IR contraction in the <i>Adenophora stricta</i> chloroplast genome.</p

Comparison of the LSC, IR, and SSC junction positions in the eight Campanuloid chloroplast genomes.

<p>Comparison of the LSC, IR, and SSC junction positions in the eight Campanuloid chloroplast genomes.</p

Comparison of chloroplast genome features of three <i>Adenophora</i> species.

<p>Comparison of chloroplast genome features of three <i>Adenophora</i> species.</p

Comparison of the Pi value in four <i>Adenophora</i> species.

<p>(A) Coding genes and (B) Non-coding regions.</p

The gene order changes in the LSC regions in the eight Campanuloid chloroplast genomes.

<p>The gene order changes in the LSC regions in the eight Campanuloid chloroplast genomes.</p