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京都大学0048新制・課程博士博士(農学)甲第10886号農博第1392号新制||農||888(附属図書館)学位論文||H16||N3897(農学部図書室)UT51-2004-G733京都大学大学院農学研究科食品生物科学専攻(主査)教授 安達 修二, 教授 井上 國世, 教授 北畠 直文学位規則第4条第1項該当Doctor of Agricultural ScienceKyoto UniversityDA

Isomerization and epimerization of glucose and galactose in arginine solution and phosphate buffer under subcritical fluid conditions

Reaction of glucose or galactose was performed in arginine solution or phosphate buffer (pH 7.0) using a batch reactor at 110°C. The yields of products, pH, and absorbances at 280 and 420 nm were measured during the reaction. Fructose, mannose, and allulose were formed from glucose; tagatose, talose, and sorbose were done from galactose. The reaction proceeded more rapidly in arginine solution than in phosphate buffer. In arginine solution, yields of fructose and tagatose were 20% and 16%, respectively, after 30-min reaction; in phosphate buffer, they were 14% and 10%, respectively. However, in both reaction media, the pH drop and increase in absorbances continued even after the yield became almost constant. The absorbance increased particularly in the latter half of the reaction due to formation of browning products. Therefore, to avoid browning, the reaction should be stopped as soon as possible after the yield approaches its maximum value

Properties of Extract from Okara by Its Subcritical Water Treatment

Okara was treated with subcritical water at temperatures ranging from 170 to 260°C for various times. After clarification, the extracts were analyzed for their protein and carbohydrate contents, DPPH radical scavenging activity, and antioxidative activity. The carbohydrate content significantly decreased with the increasing treatment temperature and time. The protein content, however, increased with the increasing treatment temperature and slightly decreased with a heating time longer than 10 min. The extract obtained from the subcritical water treatment at 240°C for 5 min, which would be used to evaluate the antioxidative activity, provided the relatively highest radical scavenging activity and the activity tended to decrease with the prolonging heating time and temperature. The extract also exhibited a suppressive activity to the autoxidation of linoleic acid with the increasing weight ratio of the extract to linoleic acid. The results clearly showed okara still contained highly valuable substances for human consumption

Decomposition kinetics of glucose and fructose in subcritical water containing sodium chloride

The kinetics of the decomposition and isomerization of glucose and fructose in pure water and water containing sodium chloride (1–20 % w/w) under subcritical conditions at 180–220 °C was investigated. The addition of sodium chloride in subcritical water accelerated the decrease of glucose, and the rate was expressed by the Weibull equation. Although the isomerization of glucose to fructose was observed in parallel with decomposition, the yield of fructose was lower at higher sodium chloride concentrations. Mannose was also formed from glucose with very low yield. It was seen that fructose decomposed much faster than glucose, in pure and salty subcritical water. The decomposition of fructose obeyed first-order kinetics in the initial stages of the reaction and could be expressed by the autocatalytic model in the later stages. The formation of glucose and mannose from fructose was not observed under any of the conditions investigated

Osmotic Dehydration, Drying Kinetics, and Quality Attributes of Osmotic Hot Air-Dried Mango as Affected by Initial Frozen Storage

Using frozen mango for osmotic hot air drying is still uncommon due to a lack of knowledge on the effect of the freezing process on the final product’s quality attributes. This study aimed to investigate the effect of the freezing method (slow and quick freezing) and frozen storage time at −18 °C (0, 1, and 2 months) on mass transfer kinetics during osmotic dehydration, drying kinetics during hot air drying, and final quality attributes of the dried mango. The results indicated that Peleg’s model could describe the water loss and solid gain during the osmotic dehydration in a 38° Brix sugar solution. Freezing before osmotic dehydration reduced the water loss rate while increasing the solid uptake content. Frozen mangoes showed slightly higher drying rates at 50 and 60 °C than the fresh ones. Freezing and frozen storage also retarded the browning reaction and polyphenol oxidase activities. The osmotic-dried mango obtained from frozen mangoes showed a chewy and gummy texture, which could be considered a distinctive texture characteristic for dried mango

Compositions, flavour and antiradical properties of products from subcritical water treatment of raw Isada krill

Comparative analysis of subcritical water (SCW) treatment and ambient pressure boiling one of raw Isada krill was performed for the nutritive, flavour and antiradical properties of krill extracts and residues as well as the molecular mass and odour intensity of the extract. SCW treatment was performed for 10 min in a batch-type vessel in the temperature range of 100–240 °C, using a 1:1 weight ratio of raw krill to water. Higher protein and lipid contents were obtained by SCW treatment relative to boiling. The lipid content of the SCW extracts increased with increasing treatment temperature. Protein was the main component in these extracts, and the highest protein content was achieved by SCW treatment at 200 °C. High-molecular-mass species decomposed under SCW treatment at high temperatures with consequent generation of smaller molecules. The antiradical activity of the SCW extract, determined by DPPH and ABTS assays, increased with increasing treatment temperature. The krill extracts and residues exhibited shrimp-like flavour, and the most desirable flavour was obtained by SCW treatment at 140 °C or 160 °C for 10 min. The treatment would be applicable for the production of seasonings from Isada krill

Subcritical Water Treatment for Producing Seasoning From Semidried Isada Krill

Semidried Isada krill (Euphausia pacifica) was treated with boiling water and subcritical water at 100–240C for 10 min with a semidried krill to a water ratio of 8/52 w/v to produce seasoning. The protein concentration, lipid content and odor concentration of the extract increased after increasing the treatment temperature. The highest protein content of the extract (0.047–0.049 kg/kg-extract) was obtained by subcritical water treatment at 180 or 200C. Compounds with lower molecular mass were detected in the extracts derived from subcritical water treatment at temperatures higher than 160C. The extract prepared by subcritical water treatment at 160 and 180C received the high score in the flavor preference test by a group of panelists. Treatment at temperatures lower and higher than 160–180C, respectively, conferred undesirable fishy and smoky or burnt odors to the extracts and residues

Degradation of Caffeic Acid in Subcritical Water and Online HPLC-DPPH Assay of Degradation Products

Caffeic acid was subjected to degradation under subcritical water conditions within 160–240 °C and at a constant pressure of 5 MPa in a continuous tubular reactor. Caffeic acid degraded quickly at these temperatures; the main products identified by liquid chromatography-diode array detection/mass spectrometry were hydroxytyrosol, protocatechuic aldehyde, and 4-vinylcatechol. The reaction rates for the degradation of caffeic acid and the formation of products were evaluated. Online high-performance liquid chromatography/2,2-diphenyl-1-picryhydrazyl assay was used to determine the antioxidant activity of each product in the solution. It was found that the overall antioxidant activity of the treated solution did not change during the degradation process. This study showed a potential of formation of antioxidants from natural phenolic compounds under these subcritical water conditions, and this may lead to a discovering of novel antioxidants compounds during the extraction by this technique