Mortality of Anthrax Toxins^*

*<p>n = the number of animals used in each dose study</p

Survival of Sprague Dawley rats (250 to 300 g) treated with single intravenous bolus infusion of LeTx (0.03 mg PA+0.015 mg LF) or EdTx (0.15 mg PA+0.075 mg EF) and monitored for two weeks.

<p>These are the same doses used for telemetry and ECHO experiments. N = 12 for each experiment. Kaplan-Meier graphs prepared.</p

Boxplots at different times post anthrax toxin bolus iv infusion of echocardiographic parameters:

<p>A. LeTx treated rats at zero, one and two hr post-infusion, velocity of propagation was 24±5, 34±7 and 46±17 cm/sec, respectively, with significant difference (P = 0.05 at one hr compared to controls and P = 0.007 at two hr compared to controls); B. LeTx treated rats at zero and one hr post-infusion, left ventricular diastolic area was 0.98±0.07 and 1.15±0.06, respectively, with significant difference (P = 0.01); C. LeTx treated rats at zero and one hr post-infusion, left ventricular systolic area was 0.79±0.08 and 0.98±0.10 cm, respectively, with significant difference (P = 0.02); D. EdTx treated rats at zero, one and two hr post-infusion, heart rate was 326±49, 371±28, and 393±10 beats per min, respectively, with significant difference (P = 0.05 at one hr and P = 0.001 at two hr compared to controls). The box represents the middle 50% of the data. The line through the box represents the median. The line (whiskers) extending from the box represent the upper and lower 25% of the data. The line of each plot connects the means of the sample.</p

Serum levels of PA after single bolus intravenous infusions of different doses of LeTx to Sprague Dawley rats (250 to 300 g).

<p>Concentrations of toxin components measured by ELISA as described in the text. Peak concentrations and half-lives shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0000466#pone-0000466-t002" target="_blank">Table 2</a>.</p

Anthrax toxins in serum samples^*

*<p>Three animals were used in each dose study; ND, not determined.</p