14 research outputs found

    Optimization of Energy Consumption in The Residential Sector, Using Automated Control Systems

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    With limited energy resources for sustainable and balanced national development it is becoming more and more important to solve tasks of optimization of energy consumption. This optimization is important not only for major Russian companies (which provide major share of tax inflows for the state budget), but also for homes in order toreduce the energy consumption. This article discusses a complex approach in the field monitoring and energy management of apartment buildings as part of the energy management program using an automated thermal and electrical energy management system developed by NGO VEST in Tomsk. It is shown that the power monitoring data allow to make economically effective decisions in reducing energy consumption costs in residential buildings. The creation of the Analytical Center and the use of an automated energy management system give the opportunity to reduce costs of heat consumption in apartment buildings and, as a consequence, increase the efficiency of use of budgetary funds, used for subsidizing the northern regions of Russia

    SELECTIVE PROCESSING OF CT IMAGES OF TRABECULAR BONE TISSUE IN THE PRESENCE OF ARTIFACTS IN THE FORM OF SUBMERSIBLE METAL STRUCTURES

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    Computed tomography is one of the informative methods of non-invasive diagnosis. Identifying artifacts can make them completely unsuitable for medical diagnosis. This work is devoted to the identification of CT-image artifacts and the search for methods for their elimination

    Synthetic gene for the hepatitis C virus nucleocapsid protein.

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    A synthetic gene encoding the hepatitis C virus (HCV) nucleocapsid protein was constructed and expressed in E. coli. To synthesize this gene, we developed a new method that results in the enzymatic synthesis of long polydeoxyribonucleotides from oligodeoxyribonucleotides. The method, designated as the 'Exchangeable Template Reaction' (ETR), uses oligonucleotides as templates for DNA polymerase. A special mechanism was designed to exchange the templates during the polymerase reaction. The mechanism relies on the formation of a single-stranded 3'-protrusion at the 'growing point' of the elongating DNA such that it can be subsequently annealed, in a sequence-specific manner, with the next synthetic oligonucleotide. When annealed to the 3'-protrusion, the added oligonucleotide becomes a template for DNA polymerase, and the protruding 3'-end of the double-stranded DNA is used as the primer. The HCV nucleocapsid gene was assembled with DNA ligase from three fragments synthesized by ETR. The data verify that this method is efficient. The main advantage of ETR is the ability to combine more than two oligonucleotides in one tube together with polymerase and an enzymatic activity that produces a 3'-protrusion (e.g., BstXI) rather than the sequential addition of each component. The data demonstrate that as many as five oligonucleotides can be used simultaneously, resulting in a synthesized DNA fragment of designed sequence. The synthetic gene expressed in E. coli produced a 27 kDa protein that specifically interacted with antibodies from sera obtained from HCV-infected individuals
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