qPCR multiplex detection of microRNA and messenger RNA in a single reaction.

Reverse Transcription-Quantitative PCR (RT-qPCR) is one of the standards for analytical measurement of different RNA species in biological models. However, current Reverse Transcription (RT) based priming strategies are unable to synthesize differing RNAs and ncRNAs especially miRNAs, within a single tube. We present a new methodology, referred to as RNAmp, that measures in parallel miRNA and mRNA expression. We demonstrate this in various cell lines, then evaluate clinical utility by quantifying several miRNAs and mRNA simultaneously in sera. PCR efficiency in RNAmp was estimated between 1.8 and 1.9 which is comparable to standard miRNA and random primer RT approaches. Furthermore, when using RNAmp to detect selected mRNA and miRNAs, the quantification cycle (Cq) was several cycles lower. This low volume single-tube duplex protocol reduces technical variation and reagent usage and is suitable for uniform analysis of single or multiple miRNAs and/or mRNAs within a single qPCR reaction

Cascading Cosmology

We develop a fully covariant, well-posed 5D effective action for the 6D cascading gravity brane-world model, and use this to study cosmological solutions. We obtain this effective action through the 6D decoupling limit, in which an additional scalar degree mode, \pi, called the brane-bending mode, determines the bulk-brane gravitational interaction. The 5D action obtained this way inherits from the sixth dimension an extra \pi self-interaction kinetic term. We compute appropriate boundary terms, to supplement the 5D action, and hence derive fully covariant junction conditions and the 5D Einstein field equations. Using these, we derive the cosmological evolution induced on a 3-brane moving in a static bulk. We study the strong- and weak-coupling regimes analytically in this static ansatz, and perform a complete numerical analysis of our solution. Although the cascading model can generate an accelerating solution in which the \pi field comes to dominate at late times, the presence of a critical singularity prevents the \pi field from dominating entirely. Our results open up the interesting possibility that a more general treatment of degravitation in a time-dependent bulk, or taking into account finite brane-thickness effects, may lead to an accelerating universe without a cosmological constant.Comment: [v2] 27 pages, 2 figures, corrected typos, expanded discussion of late-time cosmological behavio

Cascading Gravity is Ghost Free

We perform a full perturbative stability analysis of the 6D cascading gravity model in the presence of 3-brane tension. We demonstrate that for sufficiently large tension on the (flat) 3-brane, there are no ghosts at the perturbative level, consistent with results that had previously only been obtained in a specific 5D decoupling limit. These results establish the cascading gravity framework as a consistent infrared modification of gravity.Comment: 24 pages, minor changes, argument on vector perturbations improve

Weighing Neutrinos with Galaxy Cluster Surveys

Large future galaxy cluster surveys, combined with cosmic microwave background observations, can achieve a high sensitivity to the masses of cosmologically important neutrinos. We show that a weak lensing selected sample of ~100,000 clusters could tighten the current upper bound on the sum of masses of neutrino species by an order of magnitude, to a level of 0.03 eV. Since this statistical sensitivity is below the best existing lower limit on the mass of at least one neutrino species, a future detection is likely, provided that systematic errors can be controlled to a similar level.Comment: 4 pages, 1 figure, version accepted for publication in PR

A PRELIMINARY ELECTROMYOGRAPHIC INVESTIGATION INTO SHOULDER MUSCLE ACTIVITY IN CRICKET SEAM BOWLING

2 The aim of this investigation was to describe and compare surface electromyographic activity of shoulder musculature during cricket seam bowling between two elite bowlers with (bowler A) and without (bowler B) shoulder pathology. Activity of seven muscles were recorded at 500 Hz with a digital camera sampling at 210 Hz used to define phases within the movement. Whilst both the duration of the movement and ball velocity were similar between bowlers (bowler A: duration = 0.89 ± 0.04 s, ball velocity = 27.08 ± 1.21 m.s-1; bowler B: duration = 0.72 ± 0.02 s, ball velocity = 26.59 ± 1.49 m.s-1), variations in muscle activity particularly for biceps brachii and infraspinatus were established. Further research utilising larger sample sizes is required to establish if such variations occur as a consequence of shoulder pathology or if these are due to other contributing factors

A Mobile Transient Internet Architecture

This paper describes a new architecture for transient mobile networks destined to merge existing and future network architectures, communication implementations and protocol operations by introducing a new paradigm to data delivery and identification. The main goal of our research is to enable seamless end-to-end communication between mobile and stationary devices across multiple networks and through multiple communication environments. The architecture establishes a set of infrastructure components and protocols that set the ground for a Persistent Identification Network (PIN). The basis for the operation of PIN is an identification space consisting of unique location independent identifiers similar to the ones implemented in the Handle system. Persistent Identifiers are used to identify and locate Digital Entities which can include devices, services, users and even traffic. The architecture establishes a primary connection independent logical structure that can operate over conventional networks or more advanced peer-to-peer aggregation networks. Communication is based on routing pools and novel protocols for routing data across several abstraction levels of the network, regardless of the end-points’ current association and state. The architecture also postulates a new type of network referred to as the Green Network. The Green Network has protocols to coordinate routing traffic and to allow for the identification and authentication of devices, services, users and content characterized as Digital Entities. Transmission is assumed to initiate and terminate at transient physical locations. The network implements every reasonable effort to coordinate a prompt delivery to the transient end-points using whatever means available. This paper is a conceptual logical model of the intended architecture and specifics about its particular components and their implementations will be discussed in future papers

Expression of microRNAs in HPV negative tonsil cancers and their regulation of PDCD4

© 2016. Global rates of tonsil cancer have been increasing since the turn of the millennia, however we still have a limited understanding of the genes and pathways which control this disease. This array dataset which is linked to our publication (Zhang et al., 2015) describes the profiling of human miRNAs in tonsil and normal adjacent tissues. With this dataset, we identified a list of microRNA (miRNA) which were highly over represented in tonsil cancers and showed that several miRNAs were able to regulate the tumour suppressor PDCD4 in a temporal manner. The dataset has been deposited into Gene Expression Omnibus (GSE75630)

The IR-Completion of Gravity: What happens at Hubble Scales?

We have recently proposed an "Ultra-Strong" version of the Equivalence Principle (EP) that is not satisfied by standard semiclassical gravity. In the theory that we are conjecturing, the vacuum expectation value of the (bare) energy momentum tensor is exactly the same as in flat space: quartically divergent with the cut-off and with no spacetime dependent (subleading) ter ms. The presence of such terms seems in fact related to some known difficulties, such as the black hole information loss and the cosmological constant problem. Since the terms that we want to get rid of are subleading in the high-momentum expansion, we attempt to explore the conjectured theory by "IR-completing" GR. We consider a scalar field in a flat FRW Universe and isolate the first IR-correction to its Fourier modes operators that kills the quadratic (next to leading) time dependent divergence of the stress energy tensor VEV. Analogously to other modifications of field operators that have been proposed in the literature (typically in the UV), the present approach seems to suggest a breakdown (here, in the IR, at large distances) of the metric manifold description. We show that corrections to GR are in fact very tiny, become effective at distances comparable to the inverse curvature and do not contain any adjustable parameter. Finally, we derive some cosmological implications. By studying the consistency of the canonical commutation relations, we infer a correction to the distance between two comoving observers, which grows as the scale factor only when small compared to the Hubble length, but gets relevant corrections otherwise. The corrections to cosmological distance measures are also calculable and, for a spatially flat matter dominated Universe, go in the direction of an effective positive acceleration.Comment: 27 pages, 2 figures. Final version, references adde

Structure Of The Photochemical Reaction Path Populated Via Promotion Of Cf2i2 Into Its First Excited State

The photochemical reaction path following the promotion of CF2I2 into its lowest-lying excited electronic singlet state has been modeled using ab initio multiconfigurational quantum chemical calculations. It is found that a conical intersection drives the electronically excited CF2I2* species either to the CF2I + I radical pair or back to the starting CF2I2 structure. The structures of the computed relaxation pathways explain the photoproduct selectivity previously observed in the gas phase. Furthermore, the results provide the basis for explaining the condensed-phase photochemistry of CF2I2