Genetic relationships among collections of the Persian sturgeon, Acipenser persicus, in the south Caspian Sea detected by mitochondrial DNA–Restriction fragment length polymorphisms

In the present study, mitochondrial DNA polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay was used to assess the population structure and genetic relationships among six Persian sturgeon, Acipenser persicus populations from the south Caspian Sea along the Iranian coast. The complete nucleotide dehydrogenase subunit 5 (NADH 5) region of mtDNA amplified by PCR was digested with five restriction enzymes. In total, 154 individuals from six populations including: Guilan (Zone1-2), Mazandaran (Zone 3 and 5), Golestan (Zone 4) and Sefidroud River, from the south Caspian Sea along the Iranian coast were analyzed using five restriction endonucleases (Rsa І, Hinf І, HaeIII, Mbo І and Cfr13І), yielding 17 haplotypes. Samples from Sefidroud River were clearly identified by cluster and molecular variance model (AMOVA) analyses. This collection showed dominant haplotypes that were little in populations from the other geographic areas. The mean haplotype diversity (h) and nucleotide diversity (π) were 0.739±0.038 and 0.0105±0.0043, respectively. Based on heterogeneity test haplotype frequencies of Persian sturgeon populations and Monte-Carlo with 1000 replicates in PCR-RFLP method significant differences were seen (χ2 =37.12, P< 0.0001) and these results showed that haplotype distribution in different location were significant and populations of Sefidroud River were statistically significant (P< 0.0001). This result suggests that the unique genetic structure of Sefidroud River represents a highly valuable genetic resource and should now be treated as demographically independent and managed separately

Genetic diversity in the Persian sturgeon, Acipenser percicus, from the south Caspian Sea based on mitochondrial DNA sequences of the control region

The Persian sturgeon, Acipenser persicus (Borodin, 1897), is an economically important species, which mainly inhabits the Caspian Sea. However, little is known about its population genetic structure. In this study, variation in nucleotide sequences of the mitochondrial DNA (mtDNA) control region of wild stock Persian sturgeon was determined to assess the genetic diversity among different natural populations of this species. The fish (n = 46) were collected from four sites (Astara, Sefidrood, Noshahr and BandareTurkaman) in the south Caspian Sea. As a result 6 haplotypes and 44 variable sites were found. The average haplotype diversity (h) and nucleotide diversity (π) were 0.640±0.028 and 0.0442±0.011, respectively. Analysis of molecular variance (AMOVA) demonstrated that most variations occurred within samples, and the difference between the populations from Astara and Noshahr or Bandare- Turkaman was not significant (p <0.001). Estimates of gene flow indicated reproductive isolation between the Sefidrood River population and the other collections. The divergence might be related to geographical isolation. The results are consistent with the findings from PCR-RFLP analysis (PCR-RFLP) and suggest considerable genetic diversity of the population from Sefidrood River

Effects of zeolite levels on growth indexes of juvenile freshwater crayfish (Astacus leptodactylus)

To decrease cost of the commercial formulated food in culture of freshwater crayfish (Astacus leptodactylus), inexpensive additives like Zeolite can be incorporated into food. An eight week experiment was conducted on juvenile freshwater crayfish to determine the suitable level of Zeolite in food of the fish. Four levels of Zeolite 0.5, 1, 1.5 and 2 percent with three replications each and a digestible energy (DE) of 3500Kcal/kg diet were formulated. Five hundred and forty juvenile crayfish weighing 2 plus or minus 0.8 grams each were randomly distributed between twelve aquariums of 250 liter capacity. Juveniles were fed on pellet foods at 8 percent of body weight four times a day. Different nutritional responses in terms of weight gain (WG), Relative growth rate (RGR), food conversion ratio (FCR), protein efficiency ratio (PER) and survival rate (SR) were calculated and compared. WG, RGR and SR in treatments (1, 2, 3, 4) were not significant statistically (P>0.05) as compared to the control group

Effect of Weissella cibaria as probiotic on some on growth factors in Siberian sturgeon Acipenser baerii

The present study was designed to investigate the effect of bacteria Weissella cibaria as to probiotic on growth factors on Siberian sturgeon. The fish averaged 143.55 ± 0.04 g in weight were placed in 12 tanks for 60 days in four treatments everyone to three replicates including 0.2 g probiotic containing 107CFU / g for the first treatment (TA); 2 g probiotic containing 108CFU / g for the second treatment (TB) and 20 mg probiotic containing 109CFU / g for the third treatment (TC), and diet without probiotics for the control treatment (C), respectively and at the end of the trial meantime measurement the growth rate proceed to means Statistical analysis (ANOVA one- way- 95%) although, all probiotics-treated diets were resulted in to increases parameters feed conversation ration (FCR), specific growth rate (SGR) and protein efficiency ratio (PER), body weight index (BWI) comparing to the control group. However, no significant difference Condition Factor (CF) was observed between the treatments and group. Thus using this type of probiotics can be effective in improving the growth parameters

A New Approach to Nonsinusoidal Steady-State Power System Analysis

A new analysis method using wavelet domain for steady-state operating condition of power system is developed and introduced. Based on wavelet-Galerkin theory, the system components such as resistor, inductor, capacitor, transmission lines, and switching devices are modeled in discrete wavelet domain for the purpose of steady-state analysis. To solve system equations, they are transferred to wavelet domain by forming algebraic matrix-vector relations using the wavelet transform coefficients and the equivalent circuit is thus built for system simulation. After describing the new algorithm, two-case studies are presented and compared with the simulations in the time domain to verify the accuracy and computational performance

Coverage performance in multi-stream MIMO-ZFBF heterogeneous networks

We study the coverage performance of multiantenna (MIMO) communications in heterogenous networks (HetNets). Our main focus is on open-loop and multi-stream MIMO zero-forcing beamforming (ZFBF) at the receiver. Network coverage is evaluated adopting tools from stochastic geometry. Besides fixed-rate transmission (FRT), we also consider adaptive-rate transmission (ART) while its coverage performance, despite its high relevance, has so far been overlooked. On the other hand, while the focus of the existing literature has solely been on the evaluation of coverage probability per stream, we target coverage probability per communication link — comprising multiple streams — which is shown to be a more conclusive performance metric in multi-stream MIMO systems. This, however, renders various analytical complexities rooted in statistical dependency among streams in each link. Using a rigorous analysis, we provide closed-form bounds on the coverage performance for FRT and ART. These bounds explicitly capture impacts of various system parameters including densities of BSs, SIR thresholds, and multiplexing gains. Our analytical results are further shown to cover popular closed-loop MIMO systems, such as eigen-beamforming and space-division multiple access (SDMA). The accuracy of our analysis is confirmed by extensive simulations. The findings in this paper shed light on several important aspects of dense MIMO HetNets: (i) increasing the multiplexing gains yields lower coverage performance; (ii) densifying network by installing an excessive number of lowpower femto BSs allows the growth of the multiplexing gain of high-power, low-density macro BSs without compromising the coverage performance; and (iii) for dense HetNets, the coverage probability does not increase with the increase of deployment densities

Coverage performance of MIMO-MRC in heterogeneous networks:a stochastic geometry perspective

We study the coverage performance of multi-antenna (MIMO) communications with maximum ratio combining (MRC) at the receiver in heterogeneous networks (HetNets). Our main interest in on multi-stream communications when BSs do not have access to channel state information. Adopting stochastic geometry we evaluate the network-wise coverage performance of MIMO-MRC assuming maximum signal- to-interference ratio (SIR) cell association rule. Coverage analysis in MIMO-MRC HetNets is challenging due to inter-stream interference and statistical dependencies among streams' SIR values in each communication link. Using the results of stochastic geometry we then investigate this problem and obtain tractable analytical approximations for the coverage performance. We then show that our results are adequately accurate and easily computable. Our analysis sheds light on the impacts of important system parameters on the coverage performance, and provides quantitative insight on the densification in conjunction with high multiplexing gains in MIMO HetNets. We further observe that increasing multiplexing gain in high- power tier can cost a huge coverage reduction unless it is practiced with densification in femto-cell tier