高感度ウイルス検出に向けたシグナル増幅型イムノアッセイの開発

博士（工学）創造科学技術大学院静岡大学甲第1235号ET

DEVELOPMENT OF SIGNAL AMPLIFIED IMMUNOASSAY FOR ADVANCED VIRUS DETECTION

博士（工学）創造科学技術大学院静岡大学甲第1235号ET

Signal-amplified surface-enhanced Raman scattering using core/shell satellite nanoparticles for norovirus detection

The development of an innovative approach is explored to amplify the signal of a surface-enhanced Raman scattering (SERS)-based detection system using a novel nanotag: Au@Ag NPs covered by satellite AuNPs and conjugated by 4-mercaptbenzoic acid (4-MBA) as a Raman tag (Au@Ag-MBA-AuNPs). The Au@Ag-MBA-AuNPs nanotags showed strong SERS activities with an enhancement factor in the 108 order of magnitude. This indicates the formation of many hot spots due to the combination of core–shell nanoparticles and satellite AuNPs on the surface of Au@Ag-MBA NPs. The newly fabricated nanotags were employed in a small-sized Palmtop Raman spectrometer. A concentration-dependent increase in SERS intensity was observed in the norovirus-like particle (NoV-LP) concentration range 10 fg/mL to 100 pg/mL with a detection limit of 0.76 fg/mL. Even in the severe interfering matrices, this detection method's coefficient of variation was less than 10%. This detection system was approximately 107 times more sensitive than commercially available ELISA kits. Norovirus in clinical samples was detected over a wide concentration range of 1.0 × 101 – 1.0 × 106 RNA copy number/mL with a detection limit of 7.8 RNA copy number/mL, indicating sensitivity comparable to real-time PCR. These results suggest that this detection system is stable in a complex matrix and has the potential for detecting norovirus in clinical samples with a small Palmtop Raman spectrometer

L-histidine doped CDs from Zingiber Montanum using hydrothermal method to enhance its antimicrobial activity and imply for latent fingerprint detection

Carbon dots (CDs) from Zingiber Montanum as a natural resource were prepared and tested as an antimicrobial activity with various types of bacteria. Then nitrogen doped carbon dots (N-CDs) were subject to obtain from the plant extract doped with L-histidine using hydrothermal method, indicating their N-CDs fluorescence intensity increases, and can be visualized more brightness under UV lamp compared with its bare CDs. Besides its antimicrobial purpose, fingerprint image is utmost known as one of the important characteristics of the crime evident, in this study, the N-CDs are then aimed to apply on various non-porous material surfaces for latent fingerprint detection. It was shown that the fine powder of N-CDs mixed with starch give more detailed minutiae compared with that from black commercial powder. The applicable fingerprint tracer using this N-CDs/starch powder shows a bit long time clear results until 90 days, indicating that the new N-CDs developed enhances much more the fingerprint performance than its usual. In addition, to confirm the antimicrobial activity the N-CDs alone were also used for such antimicrobial testing via agarose disk diffusion assay against both gram negative and gram-positive bacteria, resulted satisfactorily that this nanomaterial can be a high potential use for antibacterial activity

Advancement of capture immunoassay for real-time monitoring of hepatitis E virus-infected monkey

autho

Enhanced colorimetric detection of norovirus using in-situ growth of Ag shell on Au NPs

autho