Effect of Artemisia extract on Argulus coregoni and Lernaea cyprinacea infestation in carp fish

Different concentrations of artemisia extract were experimentally tested to treat Lernaea and Argulus infestations in common carp (Cyprinus carpio) fish with different methods. A total number of 210 carps (50 ± 10 g) were collected from private farms at Kafr El-Sheikh Governorate, Egypt, and divided into 7-groups in triplicate: G1 negative-control (apparently healthy carps), G2 positive-control (infested carps with Lernaea and Argulus), G3: infested carps treated with dipping in a NaCl solution (5g /L for 5 min. for 3 consecutive days), G4-G5: infested carps treated with dipping in a solution of artemisia extract with concentration 50% and 60% respectively, for 5 minutes, then fishes were accommodated at a recovery tank and inspected for 10 min., G6-G7: infested carps were placed in aquarium supplied with artemisia extract (3 mg/ L and 6mg/L respectively, for 15 consecutive days). The results revealed that artemisia extract had a noticeable positive effect on survival, recovery of infested carps and the parasitic elimination% which increased proportionally with increasing the concentration of the extract, whereas the best results were obtained at G5. Also, there were an improvement in serum biochemical parameters (increased total protein, albumin and globulin with reduced AST, ALT, creatinine and urea), which were proportionally improved with increasing both concentration and the duration of exposure to the extract, (especially at G7). Gross and histopathological examination and alterations of skin, gills, liver and intestine also revealed a noticeable improvement to the normal in the groups treated with artemisia extract in comparison with G2 especially G6 and G7. Consequently, application of artemisia extract is a safe and optimal method for treatment of Argulus and Lernaea parasitism in cultured carp

Effect of Vitex agnus castus L. Extract in Carp Fish Infected with Vibrio anguillarum

This work studied in vitro; the antibacterial activity of Vitex agnus-castus (VAC) extract against V. anguillarum, its minimal inhibitory and bactericidal concentrations (MIC and MBC) and detected its bioconstituents using gas chromatography-mass spectrometry (GC-MS) analysis besides evaluated its effect in vivo in carp either alone or combined with antibiotic to prevent or treat V. anguillarum infection. A total of 180 fish were divided into 6 groups; G1: negative-control, G2: V. anguillarum infected-control, G3: infected-(marbofloxacin (MAR) 10 mg/kg body weight), G4: infected-(VAC 1gm/kg ration), G5: infected-(VAC 1 gm/kg + MAR 10 mg/kg) all treatments lasted 7 days and G6: prophylactic treated-(VAC 1 gm/kg/30 day) then challenged. Results of scanning electron microscope revealed changes in bacterial morphology and loss of flagella by VAC where its MIC and MBC were 1.95 and 15.63 mg/ml respectively. Growth performance and survival rate improved in all treated groups in this descending order (G6, G4 then G5). All treatments significantly increased total protein, albumin, globulin, superoxide dismutase and decreased aspartate and alanine aminotransferases activities, malondialdehyde, urea and creatinine than G2 and mostly near to G1 in this ascending order (G3, G4 then G5). In G6, VAC protected carp from the infection and improved growth, survivability and most of blood parameters. MAR residue in fish musculature needed 4 days as a withdrawal period to be less than the maximum residue limits (MRL), while combination of VAC with MAR decreased it less than the MRL from the 1st day post treatment. Finally, VAC had a considerable antibacterial activity against V. anguillarum in both prevention and treatment assays. It can be used either alone or adding with MAR