1 research outputs found
Isolation, characterization and complete genome sequence of PhaxI: a phage of Escherichia coli O157 :H7
Bacteriophages are considered as promising biological agents for the control of infectious
diseases. Sequencing of their genomes can ascertain the absence of antibiotic resistance, toxin
or virulence genes. The anti-O157 :H7 coliphage, PhaxI, was isolated from a sewage sample in
Iran. Morphological studies by transmission electron microscopy showed that it has an
icosahedral capsid of 85–86 nm and a contractile tail of 115�15 nm. PhaxI contains dsDNA
composed of 156 628 nt with a G+C content of 44.5 mol% that encodes 209 putative proteins.
In MS analysis of phage particles, 92 structural proteins were identified. PhaxI lyses Escherichia
coli O157 :H7 in Luria-Bertani medium and milk, has an eclipse period of 20 min and a latent
period of 40 min, and has a burst size of about 420 particles per cell. PhaxI is a member of the
genus ‘Viunalikevirus’ of the family Myoviridae and is specific for E. coli O157 : H7