Plant Nanobionics and Its Applications for Developing Plants with Improved Photosynthetic Capacity

In the present scenario, the ever-growing human population, a decreasing availability of land resources and loss of agricultural productivity are the major global concerns, and these possess a challenge for scientific community. To feed the increasing world population, an increase in the crop productivity with available land resources is one of the essential needs. Crop productivity can be increased by engineering the crop plants for tolerance against various environmental stresses and improving the yield attributes, especially photosynthetic efficiency. Nanomaterials have been developed with new functional properties like improved solar energy harvest. With these nanomaterials, nanobionic plants were developed by the facilitated kinetic trapping of nanomaterials within photosynthetic organelle, that is, chloroplast. The trapping of nanomaterials/nanotubes improved chloroplast carbon capture, that is, photosynthesis by improving chloroplast solar energy harnessing and electron transport rate. Besides improving photosynthesis, nanotubes like poly(acrylic acid) nanoceria (PAA-NC) and single-walled nanotube-nanoceria (SWNT-NC) decrease the amount of reactive oxygen species (ROS) inside extracted chloroplast and influence the sensing process in plants, and these are beneficial for a number of physiological processes. The nanobionic approach to engineer plant functions would lead to an era of plant research at the interface of nanotechnology and plant biology. In this chapter, nanobionic approach, transfer of nanomaterial to plants and their offspring and its potential applications to improve photosynthesis will be discussed

Spindle Cell Variant of Medullary Carcinoma Thyroid- A Case Report

A 14-year-old girl presented with a solitary thyroid nodule since 1 year. Fine needle aspiration cytology (FNAC) from the thyroid swelling revealed discretely arranged spindle cells with scant cytoplasm. Because of diagnostic dilemma, a provisional diagnosis of spindle cell lesion of thyroid was given as distinction of amyloid from collagen and basement-like material was difficult on cytology smears. Also, spindle cell might be seen in anaplastic carcinoma, melanoma, soft tissue sarcomas, etc. Tumor cells were positive for cytokeratin and chromogranin in cell block preparation. Thus, the diagnosis of spindle cell variant of medullary carcinoma thyroid was given

Functional genomic and transformation resources for commercially important red macroalgae (Rhodophyta)

Red macroalgae underpin many commercially important food, pharmaceutical and other important industries. To date, research into these species has generally focused on improving seaweed cultivation, developing new methods to extract useful compounds, or identify novel applications. Due to their economic importance, there is a requirement to develop a more complete understanding of the genome and metabolic pathways in these key seaweed species. This review describes progress in genomics, transcriptomics, protoplast isolation, and transformation approaches. It also explores the potential of genome editing using the CRISPR/Cas system to further our understanding of gene function related to different metabolic pathways and resolving unexplored aspects of macroalgal physiology traits linked to crop improvement. The application of functional genomics is essential to gain a complete understanding of both physiological and metabolomic processes, that will ultimately enhance the commercial resilience of macroalgae related industries that are subject to numerous pressures, including climate change. Although the use of genetic manipulation to alter growth characteristics or composition in seaweed will not readily apply to the macroalgae industry in the short term, it is likely to be critical for sustaining future commercial growth. The functional characterisation of macroalgal genes through the CRISPR/ Cas approach promises to open new avenues for translational research on utilising macroalgal resources for the sustainable development of these aquaculture systems

Effect of salinity and nitrogen fertilization levels on growth parameters of Sarcocornia fruticosa, Salicornia brachiata, and Arthrocnemum macrostachyum

Salinity negatively influences crop growth, but several salt-tolerant plant species (halophytes) are viable crops. Sarcocornia fruticosa (ecotypes EL and VM) is currently cultivated, but there is demand for new crop candidates and higher biomass production. Salicornia brachiata Roxb. and Arthrocneum macrostachyum L. are considered novel crops, and to realize their potential, their response to salinity and nitrogen nutrition was compared to S. fruticosa ecotypes. Experiments revealed that higher N supplemented with lower NaCl significantly increased fresh and dry shoot biomass. Lower biomass was obtained at lower nitrogen supplemented with elevated NaCl, whereas total soluble solids content positively correlated with NaCl fertigation in both Sarcocornia ecotypes. Protein content increased with a lower nitrogen supply. Anthocyanins and oxygen radical absorbance capacity were highest in S. fruticosa EL and A. macrostachyum at higher NaCl supply. The results show that halophytes have a variety of strategies to cope with high NaCl, even between ecotypes of the same species. Notably, repetitive harvesting of S. brachiata delayed flowering enabling year-round biomass production. Additionally, S. brachiata accumulated higher biomass than Sarcocornia VM when grown in a greenhouse at higher radiation than in a growth room and strongly supports its inclusion as a cash-crop halophyte.info:eu-repo/semantics/publishedVersio

A comparative study of analgesic activity of Plumbago zeylanica Linn. callus and root extracts in experimental mice

ABSTRACT Plumbago zeylanica Linn. (Plumbaginaceae) commonly known as 'Chitrak', is a perennial subscandant shrub and is listed as threatened medicinal plant. The roots of the plant were used traditionally as an abortifacient, germicidal and in treatment of liver disease, cancer, body pain, fever and inflammation. Since it is an threatened and potential medicinal plant therefore it is of great interest to evaluate the analgesic effect of callus developed by nodal explant and to compare its action with respect to root extract of parent plant. Dried callus and roots from parent plant were powdered and extracted with ethanol. The callus extract (PCE) and root extract (PRE) at dose 100,200 and 400 mg/kg were evaluated for peripheral and central analgesic activity by glacial acetic acid induced writhing and tail immersion model respectively. PRE significantly (P<0.01) reduced the writhing count at 200 mg/kg whereas PCE alters the pain threshold significantly at 400 mg/kg. In tail immersion model, PRE increase the reaction time significantly (P<0.01) at 400 mg/kg. while PCE failed to alters the reaction time significantly throughout the observation period (upto 3 hrs). In conclusion, a massive light creamish brown and granular callus formed with MS M\medium supplemented with naphthalene acetic acid (1.5 ppm) and kinetin (0.25 ppm) and it possess a significant peripheral analgesic activity

Comparison of analgesic efficacy of perineural dexamethasone in rectus sheath block and intravenous dexamethasone with levobupivacaine in bilateral rectus sheath block for patients undergoing midline abdominal surgery: a randomized controlled trial

Abstract Background Dexamethasone is used in regional analgesia to prolong the duration of analgesia and anaesthesia. The perineural and intravenous (IV) dexamethasone in fascial plane block have conflicting results. The study aimed to compare analgesic efficacy of perineurally and intravenously dexamethasone in rectus sheath block (RSB). This prospective, double-blinded, randomized control study was conducted in 105 patients, equally divided in three groups. All the patients received ultrasound-guided RSB block with 20 ml of 0.25% levobupivacaine on each side. Additionally, in group A, 1-ml normal saline (NS) on each side in RSB block and 2-ml NS IV; in group B, 4-mg dexamethasone on each side in RSB block and 2-ml NS IV; and in group C, 1-ml NS on each side in RSB block and 8-mg dexamethasone IV. The primary objective of the study was the duration of analgesia, and secondary objectives were total morphine consumption in the first 24 h, numeric rating scale (NRS) scores at rest and on cough, complications and patient’s satisfaction score. Results The duration of analgesia was maximum in group B (935.91 ± 121.82 min) and then in group C (730.31 ± 129.64 min) and group A (418.34 ± 29.22 min) (P < 0.0001). The morphine consumption and mean NRS score (at rest and cough) were lowest in group B and then group C and group A (P < 0.0001). Conclusions The perineural dexamethasone as an adjuvant to levobupivacaine prolongs the duration of analgesia with decreased analgesic requirements compared to intravenous dexamethasone in bilateral RSB in patients undergoing midline laparotomy surgery