Immunofluorescence for GFP and NeuN.

<p>A–C shows the results form an animal that did not receive any TMP in the drinking water. D–F shows the results from an animal that received 0.1 mg/ml TMP in the drinking water for 3 weeks. Note the large number of GFP positive cells in the injected striatum. The squares at the bottom right show enlargements of colabeled cells, arrows indicate examples. A,D: GFP staining; B,E; NeuN staining; C, F: overlay.</p

Dose-response histogram for YFP regulation.

<p>The number of YFP expressing cells in the striatum increases in correlation to increasing TMP doses. The number of GFP expressing cells from a non-regulated GFP control vector is also included in the histogram. There were no significant differences between the numbers of GFP expressing cells in the 0.1 and 02 mg/ml TMP groups compared to the control Lv.</p

Vector details and experimental design.

<p>(A) A schematic view of the vectors used in the present study. All transgenes were expressed from the human CMV promoter, a central poly-purine tract (cPPT) was included as well as the post-transcriptional regulatory element WPRE. The destabilizing domain (DD) was placed either downstream or upstream of the yellow fluorescent protein (YFP). A vector expressing unregulated enhanced green fluorescent protein (GFP) was used as control. (B) Shows the different experimental designs. All groups included 5 animals.</p

Regulating GDNF expression using DD.

<p>(A) Histogram showing the levels of GDNF produced from DD-YFP vector, wild type GDNF, GDNF-DD and DD-GDNF, as measured by GDNF ELISA. C-terminal fusion of the DD to GDNF did not result in any GDNF production, while N-terminal DD-GDNF fusion resulted in TMP-induced GDNF production. Furthermore, the DD-GDNF was functional in that it induced tyrosine hydroxylase expression in the TGW cell line as shown in (B).</p

Regulation of YFP by destabilizing domains in vivo.

<p>Histograms showing the number of YFP expressing cells in the transduced striatum at 3 weeks (A) and 6 weeks (B) post injections. Addition of 0.1 mg/ml TMP to the drinking water induced expression of transgene expressing cells in all animals. The effect was not dependent on whether the DD was fused to the N- or C- terminus of YFP. (C) and (D) show the dynamics of YFP protein expression 1 week or 3 weeks with or without TMP, for YFP-DD and DD-YFP, respectively. One week of adding TMP to the drinking water or omitting it was not enough to fully regulate the protein expression ,On the other hand, 3 weeks of treatment was sufficient to reach levels of YFP expression similar to those attained from chronic TMP treatment or no TMP at all, respectively. *** = <i>p</i><0.001, ** = <i>p</i><0.01.</p

Primula kisoana Miquel

原著和名: カッコサウ キソコザクラ科名: サクラソウ科 = Primulaceae採集地: 群馬県 鳴神山 (上野 鳴神山)採集日: 1963/5/5採集者: 萩庭丈壽整理番号: JH024269国立科学博物館整理番号: TNS-VS-97426

Additional file 3: Figure S3. of Astroglial NF-kB contributes to white matter damage and cognitive impairment in a mouse model of vascular dementia

Histological changes in the internal and external capsule. Reactive astrogliosis (A, E), microgliosis (B, F), axonal degeneration (C, G) and demyelination (D, H) in sham and BCAS animals in the internal capsule (upper row) and external capsule (lower row). (PDF 118 kb