Rapid and stimulus dependent discrimination times are observed in a go/no-go olfactory task under head-restrained conditions.

<p>(<b>A–B</b>) Determination of discrimination times in presence (WLS) and absence of warning stimuli (WOLS): No influence of warning stimuli (light+sound) in the DT measurements. (WOLS = Without Light and Sound, WLS = With Light and Sound). (<b>A</b>) Accuracies measured for simple and complex odor tasks of amyl acetate and ethyl butyrate, 300 trial blocks from which DT is calculated (for AA vs EB, blue bars, n = 8 mice, average ± sem; for binary mixtures, red bars, n = 8 mice, average ± sem). (<b>B</b>) DTs were increased for complex odors compared to simple odors even in the presence and absence of warning stimuli (for AA vs EB, blue bars, n = 8 mice, average ± sem; for binary mixtures, red bars, n = 8 mice, average ± sem. (<b>C–D</b>) Accuracy and discrimination times measured for cit vs but and clov vs cam, 300 trial blocks from which DT is calculated (for cit vs but, green bar and clov vs cam, brown bar, n = 8 mice, average ± sem). Dotted lines in (A) and (C) indicate chance levels.</p

Similar discrimination abilities were observed under freely moving and head-restrained conditions.

<p>(<b>A</b>) Schema depicting head-restrained and freely moving paradigms. (<b>B</b>) Discrimination accuracy shown as the average percentage of correct choices, 300 trial blocks from which DT is calculated (filled bars represent head-restrained conditions on a go/no-go task and empty bars represent freely moving conditions on a go/no-go task) for AA vs EB (blue, n = 8 mice, average ± sem), binary mixtures of AA and EB (red, n = 8 mice, average ± sem), cit vs but (green, n = 8 mice, average ± sem) and clove vs cam (brown, n = 8 mice, average ± sem). Dotted line indicates chance level. (<b>C</b>) Stimulus dependent DTs were observed for go/no-go conditioning task under freely moving conditions and were similar to the DTs measured under head-restrained conditions (color codes and number of mice: same as above, all values are expressed as average ± sem).</p

Go/no-go olfactory conditioning task under head-restrained conditions.

<p>(<b>A</b>) Mouse engaged in a go/no-go operant conditioning task. (<b>B</b>) Schema of rewarded and unrewarded trials. (<b>C</b>) Structure of a single trial. The criterion for a water reward was the total lick of 80 ms in three time bins of 500 ms out of four bins during the 2 s odor presentation, if there was no baseline licking. If mice were licking during the baseline, they had to lick double amount of time during the odor presentation to get the water reward.</p