Mechanisms underlying dual effects of serotonin during development of Helisoma trivolvis (Mollusca).

BACKGROUND: Serotonin (5-HT) is well known as widely distributed modulator of developmental processes in both vertebrates and invertebrates. It is also the earliest neurotransmitter to appear during neuronal development. In aquatic invertebrates, which have larvae in their life cycle, 5-HT is involved in regulation of stages transition including larval metamorphosis and settlement. However, molecular and cellular mechanisms underlying developmental transition in aquatic invertebrate species are yet poorly understood. Earlier we demonstrated that in larvae of freshwater molluscs and marine polychaetes, endogenous 5-HT released from the neurons of the apical sensory organ (ASO) in response to external stimuli retarded larval development at premetamorphic stages, and accelerated it at metamorphic stages. Here we used a freshwater snail Helisoma trivolvis to study molecular mechanisms underlying these dual developmental effects of 5-HT. RESULTS: Larval development of H. trivolvis includes transition from premetamorphic to metamorphic stages and shares the main features of metamorphosis with free-swimming aquatic larvae. Three types of 5-HT receptors (5-HT1-, 5-HT4- and 5-HT7-like) are functionally active at premetamorphic (trochophore, veliger) and metamorphic (veliconcha) stages, and expression patterns of these receptors and respective G proteins undergo coordinated changes during development. Stimulation of these receptors modulated cAMP-dependent regulation of cell divisions. Expression of 5-HT4- and 5-HT7-like receptors and their downstream Gs protein was down-regulated during the transition of pre- to metamorphic stage, while expression of 5-HT1 -like receptor and its downstream Gi protein was upregulated. In accordance with relative amount of these receptors, stimulation of 5-HTRs at premetamorphic stages induces developmental retardation, while their stimulation at metamorphic stages induces developmental acceleration. CONCLUSIONS: We present a novel molecular mechanism that underlies stage-specific changes in developmental tempo of H. trivolvis larvae in response to endogenous 5-HT produced by the neurons of the ASO. We suggest that consecutive changes in expression patterns of different receptors and their downstream partners in the course of larval development represent the molecular base of larval transition from premetamorphic (non-competent) to metamorphic (competent) state

Diffusive Shock Acceleration and Reconnection Acceleration Processes

Shock waves, as shown by simulations and observations, can generate high levels of downstream vortical turbulence, including magnetic islands. We consider a combination of diffusive shock acceleration (DSA) and downstream magnetic-island-reconnection-related processes as an energization mechanism for charged particles. Observations of electron and ion distributions downstream of interplanetary shocks and the heliospheric termination shock (HTS) are frequently inconsistent with the predictions of classical DSA. We utilize a recently developed transport theory for charged particles propagating diffusively in a turbulent region filled with contracting and reconnecting plasmoids and small-scale current sheets. Particle energization associated with the anti-reconnection electric field, a consequence of magnetic island merging, and magnetic island contraction, are considered. For the former only, we find that (i) the spectrum is a hard power law in particle speed, and (ii) the downstream solution is constant. For downstream plasmoid contraction only, (i) the accelerated spectrum is a hard power law in particle speed; (ii) the particle intensity for a given energy peaks downstream of the shock, and the distance to the peak location increases with increasing particle energy, and (iii) the particle intensity amplification for a particular particle energy, f(x,c/c_0)/f(0,c/c_0), is not 1, as predicted by DSA, but increases with increasing particle energy. The general solution combines both the reconnection-induced electric field and plasmoid contraction. The observed energetic particle intensity profile observed by Voyager 2 downstream of the HTS appears to support a particle acceleration mechanism that combines both DSA and magnetic-island-reconnection-related processes

Particle acceleration and reconnection in the solar wind

An emerging paradigm for the dissipation of magnetic turbulence in the supersonic solar wind is via localized quasi-2D small-scale magnetic island reconnection processes. An advection-diffusion transport equation for a nearly isotropic particle distribution describes particle transport and energization in a region of interacting magnetic islands [1; 2]. The dominant charged particle energization processes are 1) the electric field induced by quasi-2D magnetic island merging, and 2) magnetic island contraction. The acceleration of charged particles in a “sea of magnetic islands” in a super-Alfvénic flow, and the energization of particles by combined diffusive shock acceleration (DSA) and downstream magnetic island reconnection processes are discussed

Интерпретация понятия «модернизация» применительно к устойчивому развитию хозяйственного субъекта

Veral definitions of modernization "in general" were considered in order to develop an understanding of modernization at the level of a particular company (micro level). Modernization is a process of transforming a company into more modern condition characterized by the use of advanced technologies and the application of good practices of labor and production, leading to increased competitiveness. Today companies are under the influence of an ever-changing environment and increasing competitive pressures. This leads to the necessity of determining the long-term prospects, or "strategic management", and to ensure competitive advantages in the future. Companies should consider modernization as a strategy of development. The situation in Russia in the last decade requires the action of the modernization of economy by modernizing its structural units – companies, according to the statements of the top officials of the country, paying attention to the complexity of the modernization and the need to involve all sectors of the economy in the process. Additionally, because the technological backwardness leads to increased negative impacts on the environment, modernization should be considered in the context of sustainable development.Выявившаяся динамичность процесса модернизации проиллюстрирована некоторыми выска-зываниями специалистов в этой области. В современных условиях предлагается рассматривать модернизацию на микроуровне, как стратегическое направление социо-эколого-экономического устойчивого развития предприятий