sj-pdf-1-jao-10.1177_03913988231213722 – Supplemental material for Microaxial mechanical circulatory support after orthotopic heart transplantation

Supplemental material, sj-pdf-1-jao-10.1177_03913988231213722 for Microaxial mechanical circulatory support after orthotopic heart transplantation by Christopher Pritting, Danial Ahmad, Keyur Patel, Takuma Miyamoto, Taufiek K Rajab, Indranee N Rajapreyar, Howard T Massey and Vakhtang Tchantchaleishvili in The International Journal of Artificial Organs</p

Network associated with glomerular injury, metabolic disease, organismal injuries and abnormalities: Illustration of HNF4A as central gene.

<p>Network associated with glomerular injury, metabolic disease, organismal injuries and abnormalities: Illustration of HNF4A as central gene.</p

Upstream regulators predicted to be activated.

<p>Upstream regulators predicted to be activated.</p

Interferon signaling pathway cross-talk with other activated pathways in NASH.

<p>Interferon signaling pathway cross-talk with other activated pathways in NASH.</p

Top diseases and functions.

<p>Top diseases and functions.</p

Network of predicted activated upstream regulators.

<p>Network of predicted activated upstream regulators.</p

Significant pathways predicted to be activated or inhibited by z-score.

<p>A positive z-score predicts activation, a negative z-score predicts inhibition. Genes listed in Bold and <i>Italic</i> show up- and down-regulation, respectively.</p

Network of inflammatory response, glucose metabolism and lipid metabolism in NASH.

<p>Network of inflammatory response, glucose metabolism and lipid metabolism in NASH.</p

The genotoxicity of K₂CrO₄ in human and DT40 cells is drastically decreased when K₂CrO₄ incubation time is reduced.

<p>(A) <i>REV1</i> ko DT40 cells were incubated with K₂CrO₄ for 10 min to 8 hours followed by extensive washing. The cells were further cultivated for 3 days in fresh medium without addition of K₂CrO₄ to determine cell survival. (B) The exposure times (hours) were multiplicatively inversed followed by log transformation (x-axis). LC₅₀ data for each exposure time were log transformed (y-axis). Linear regression analysis was performed to determine the relationship between exposure time and LC₅₀ values (p<0.0001). (C) TK6 <i>POLD3</i> knock-down cells and TK6 mock shRNA-treated cells were incubated with K₂CrO₄ for 10 min followed by extensive washing. The cells were further cultivated for 3 days in fresh medium without addition of K₂CrO₄ to determine cell survival. The survival curves were compared between 10-min and 3-day exposure groups (p<0.0001). The green arrow indicates the Cr(VI) concentration (172,000 μg/L) that causes an increase in oral cancer, a finding that was previously shown in an NTP rodent study [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0167503#pone.0167503.ref007" target="_blank">7</a>].</p

K₂CrO₄ reduces the viability of human cells deficient in certain DNA repair pathways at Cr(VI) levels detected in U.S. city water.

<p>HeLa cells (A) and TK6 cells (B) transiently or stably knocked down with shRNA against <i>RAD54</i>, <i>BRCA1</i>, or <i>POLD3</i> were exposed to K₂CrO₄ for ~3 days to determine their survival rate. (C) Point of departure analysis of TK6 indicated significant differences (p<0.0001) for TK6 control (log10(BMD) = 1.53, exponential model) vs. <i>POLD3</i> shRNA knock down (log10(BMD) = 0.99 (9.8 μg/L), polynomial model). The black arrow indicates the maximum contaminant levels (MCL) set by the U.S. EPA. The open arrow shows the highest Cr(VI) levels detected in US city water.</p