Seven piRNAs expression profile in the porcine testes.

<p>(A) Seven piRNAs expression profile in the porcine testes from 60-day and 180-day testes of Chinese Meishan and Large White pigs using stem-loop qRT-PCR method. The X-axis represents the small RNAs and the Y-axis shows the relative expression levels of small RNAs (–ΔC_t values for qRT-PCR). The significance of differences for the expression between 60-day (sexually immature) and 180-day (sexually mature) testes of Chinese Meishan and Large White pigs was calculated using two-tailed T-test. *, p≤0.05; **, p≤0.01. (B) Seven piRNAs expressed in the porcine testes by stem-loop semi-quantitative RT-PCR. M, 25 bp DNA ladder; He, heart; Li, liver; Sp, spleen; Lu, lung; Ki, kidney; LM, <i>longissimu</i>s <i>dorsi</i> muscle; BF, backfat; Te, testis; Ov, ovary.</p

Characterization of <i>sus scrofa</i> small RNAs.

<p>(A) Length distribution of small RNAs. <i>Sus scrofa</i> small RNAs displayed a bimodal length distribution with two peaks at 21 nt and 29 nt. (B) Bar chart summarizing the annotation of small RNA populations in total RNA from testes. (C) Chromosomal distribution of small RNAs.</p

The chromosomal distribution of piRNAs and piRNA clusters in the genome.

*<p>All piRNAs with the unique perfectly mapping to pig genome were used to analyze to chromosome distribution. MT, mitochondrial genome.</p