Predicting the Identities of su(met-2) and met-3 in Neurospora crassa by Genome Resequencing

A significant number of classical genetic Neurospora crassa biochemical mutants remain anonymous, unassociated with a physical genome locus. By utilizing short read next-generation sequencing methods, it is possible to sequence the genomes of mutant strains rapidly and economically for the purpose of identifying genes associated with mutant phenotypes. We have taken this approach to connect genes and mutations to “methionineless” phenotypes in N. crassa

Circulating emm types of Streptococcus pyogenes in Scotland: 2011-2015

No abstract available

Standards for the care of people with cystic fibrosis; establishing and maintaining health

Cystic fibrosis; Health; StandardsFibrosis quística; Salud; EstándaresFibrosi quística; Salut; EstàndardsThis is the second in a series of four papers updating the European Cystic Fibrosis Society (ECFS) standards for the care of people with CF. This paper focuses on establishing and maintaining health. The guidance is produced using an evidence-based framework and with wide stakeholder engagement, including people from the CF community. Authors provided a narrative description of their topic and statements, which were more directive. These statements were reviewed by a Delphi exercise, achieving good levels of agreement from a wide group for all statements. This guidance reinforces the importance of a multi-disciplinary CF team, but also describes developing models of care including virtual consultations. The framework for health is reinforced, including the need for a physically active lifestyle and the strict avoidance of all recreational inhalations, including e-cigarettes. Progress with cystic fibrosis transmembrane conductance regulator (CFTR) modulator therapy is reviewed, including emerging adverse events and advice for dose reduction and interruption. This paper contains guidance that is pertinent to all people with CF regardless of age and eligibility for and access to modulator therapy

Enhanced balance associated with coordination training with stochastic resonance stimulation in subjects with functional ankle instability: an experimental trial

<p>Abstract</p> <p>Background</p> <p>Ankle sprains are common injuries that often lead to functional ankle instability (FAI), which is a pathology defined by sensations of instability at the ankle and recurrent ankle sprain injury. Poor postural stability has been associated with FAI, and sports medicine clinicians rehabilitate balance deficits to prevent ankle sprains. Subsensory electrical noise known as stochastic resonance (SR) stimulation has been used in conjunction with coordination training to improve dynamic postural instabilities associated with FAI. However, unlike static postural deficits, dynamic impairments have not been indicative of ankle sprain injury. Therefore, the purpose of this study was to examine the effects of coordination training with or without SR stimulation on static postural stability. Improving postural instabilities associated with FAI has implications for increasing ankle joint stability and decreasing recurrent ankle sprains.</p> <p>Methods</p> <p>This study was conducted in a research laboratory. Thirty subjects with FAI were randomly assigned to either a: 1) conventional coordination training group (CCT); 2) SR stimulation coordination training group (SCT); or 3) control group. Training groups performed coordination exercises for six weeks. The SCT group received SR stimulation during training, while the CCT group only performed coordination training. Single leg postural stability was measured after the completion of balance training. Static postural stability was quantified on a force plate using anterior/posterior (A/P) and medial/lateral (M/L) center-of-pressure velocity (COPvel), M/L COP standard deviation (COPsd), M/L COP maximum excursion (COPmax), and COP area (COParea).</p> <p>Results</p> <p>Treatment effects comparing posttest to pretest COP measures were highest for the SCT group. At posttest, the SCT group had reduced A/P COPvel (2.3 ± 0.4 cm/s vs. 2.7 ± 0.6 cm/s), M/L COPvel (2.6 ± 0.5 cm/s vs. 2.9 ± 0.5 cm/s), M/L COPsd (0.63 ± 0.12 cm vs. 0.73 ± 0.11 cm), M/L COPmax (1.76 ± 0.25 cm vs. 1.98 ± 0.25 cm), and COParea (0.13 ± 0.03 cm²vs. 0.16 ± 0.04 cm²) than the pooled means of the CCT and control groups (P < 0.05).</p> <p>Conclusion</p> <p>Reduced values in COP measures indicated postural stability improvements. Thus, six weeks of coordination training with SR stimulation enhanced postural stability. Future research should examine the use of SR stimulation for decreasing recurrent ankle sprain injury in physically active individuals with FAI.</p

Prospectus, March 11, 1976

TUITION COULD GO UP: STATE CUTS EDUCATION $; Schorr to speak?; PC news in brief: Breaker good buddy, SSU and EIU here, Health Fair March 23, 24; First time ever: President Ford comes to C-U; Students; Letters to the Editor: She is the one, What is Stu-Go?; Primaries next Tuesday; Petition to start; Use this coupon; Krigbaum Billiard Ace; McCabe, Williams honored; Three sophs awarded scholarships; Faculty Senate votes on Mid-Term grades; Vinyl Love: Run with the pack; Senate stays scholarship cuts; Scouffas joins Mugs ; Dear Bonnie; Ping Pong; Applications available; Parkland offers care course; Sign language; Ron Chrastka midnight-three; Edlefsen takes first; Interns to aid; Parkland night; Woodard V.P.; Game Tourney; Award Letter; Historical needlework: Bicentennial tapestry; Scholarships available; Respiratory Therapy: Programs approved; Skylines: Comet West; Burn your draft card, no lottery; Psych lunch and lecture; Theta raffle; Classifieds; Sports Views: Amateur - Professional; Cobra\u27s Corner: Blackie Blackwell comes to Parkland; Bouncing Bob\u27s Basketball Bonanza; Reed predicts: Our pitching is going to be strong! ; Jay Ogden All-American; Cagers Honored; Four starters to return: Women finish fifth in statehttps://spark.parkland.edu/prospectus_1976/1020/thumbnail.jp

Prospectus, March 25, 1976

CHAMPIONSHIPS BECKON P/C WINNING SPEAKERS; CBS\u27 Edwards Commencement speaker: Schorr to speak here Mar. 29; Pre-registration set for Apr. 19-30; PC news in brief: Bicentennial Week Activities, Craft Festival Day, On-Going Activities; Letters to the Editor: Peoples\u27 Forum; PC Board of Trustees Election set April 10; Vets Elect; Smith: \u27Parkland has spoken\u27; Bunnies revolutionize Stu-Go; Parkland Observes 10th Anniversary; Fiddler, Singer perform; Exhibits, spinning to be shown; Craft festival Apr. 3 at P/C; Micro Tech comes to the rescue: Clock to tick again July 4; Commager campus appearance highlight of Bicentennial week; Talents of older people on exhibit; Student Government Calendar of Events; 2-day Health Fair on campus; Debaters take third; Recycle contest; Thanks; Skylines: Stellar Origin of Life; LRC reports on latest requests: no, yes, no....; Hands; Bookstore Bulletins; Country Bouquet; Audio-Visual Dept. offers study materials; Judge speaks April 6; April 1 Meeting; Award Letter; Under hypnosis, \u27Bear\u27 bares all; Classifieds; Cobra\u27s Corner: Jim Reed, \u27Class A\u27 Coach for 5 seasons; Cobra\u27s .500 after 8 games; Emmet Fairfield wins Bouncing Bob\u27s Bonanza; Bouncing Bob\u27s Basketball Bonanza; \u2724 oz.\u27 win IM Basketball; IM Softball starts; Rooms provide needed P.E. space; 1st home game April 5th: Womens\u27 softball beginshttps://spark.parkland.edu/prospectus_1976/1019/thumbnail.jp

Monomerization of Far-Red Fluorescent Proteins

Anthozoa-class red fluorescent proteins (RFPs) are frequently used as biological markers, with far-red (λ_(em) ∼ 600–700 nm) emitting variants sought for whole-animal imaging because biological tissues are more permeable to light in this range. A barrier to the use of naturally occurring RFP variants as molecular markers is that all are tetrameric, which is not ideal for cell biological applications. Efforts to engineer monomeric RFPs have typically produced dimmer and blue-shifted variants because the chromophore is sensitive to small structural perturbations. In fact, despite much effort, only four native RFPs have been successfully monomerized, leaving the majority of RFP biodiversity untapped in biomarker development. Here we report the generation of monomeric variants of HcRed and mCardinal, both far-red dimers, and describe a comprehensive methodology for the monomerization of red-shifted oligomeric RFPs. Among the resultant variants is mKelly1 (emission maximum, λ_(em) = 656 nm), which, along with the recently reported mGarnet2 [Matela G, et al. (2017) Chem Commun (Camb) 53:979–982], forms a class of bright, monomeric, far-red FPs

Prospectus, September 28, 1976

PINS\u27 COMING SOON; Gonzalez and Steffey ineligible: McCabe, Kirkpatrick elected; 25%-33%: Drop rate high; Stewart speaks to com 111 \u27I love the business\u27; Faculty plans \u2777-\u2778 curriculum; Letters to the editor: Pins still bouncing, Oops, We\u27re waiting; Guest Editorial: Mellowspread does not mind campaign attack on Mr. Big ; Student activities provides \u27Driver exchange board\u27; Vandalizes and burns own car; Jesus Christ is alive and well: Hold everything!; Guest opinion: containers big waste; \u27Antonia\u27 on Thursday; PC news in brief: Game tourney, Joe Matthews, It\u27s debatable, Scott to speak, Peanuts popcorn candy, IOC, Soc. Assoc. wake-up, Kirk elected president, Weight control seminar; United Way needs more than donations: Walker wants community help; Tau Epsilon gleans local corn fields; Head Start Follow-Up tutor program; Nadia will dance Oct. 5; Dear Bonnie; Prairie Festival; Burgoo treat of the day; Concert review: Basie Band swings, Williams sings; \u27Birthday Party\u27 opens tomorrow; Coral Reefers back him up: Buffet a hit at auditorium; Uncle Bob\u27s review: Debate get you down?; Elvis tickets for concert sold out: Classifieds; Cobra\u27s Corner: Abbey named USCSC games committee; First home volleyball game tonight; Mudrock 1st, Cobras 6th; Bob Zettler wins Fast Freddy; Fast Freddy\u27s Football Forecast; Harriers bow to Illini JVs; Games of October 2https://spark.parkland.edu/prospectus_1976/1010/thumbnail.jp

Phase space density and chiral symmetry restoration in relativistic heavy ion collisions

The effect of altered hadron masses is studied for its effect with regard to final-state hadronic observables. It is shown that the final phase space densities of pions and kaons, which can be inferred experimentally, are sensitive to in-medium properties of the excited matter at earlier stages of the collision, but that the sensitivity is significantly moderated by interactions that change the effective numbers of pions and kaons during the latter part of the collision.Comment: 5 pages, 4 fig.