1 research outputs found
Point-of-Care Determination of Acetaminophen Levels with Multi-Hydrogen Bond Manipulated Single-Molecule Recognition (eMuHSiR)
This work aims to
face the challenge of monitoring small molecule
drugs accurately and rapidly for point-of-care (POC) diagnosis in
current clinical settings. Overdose of acetaminophen (AP), a commonly
used over the counter (OTC) analgesic drug, has been determined to
be a major cause of acute liver failure in the US and the UK. However,
there is no rapid and accurate detection method available for this
drug in the emergency room. The present study examined an AP sensing
strategy that relies on a previously unexplored strong interaction
between AP and the arginine (Arg) molecule. It was found that as many
as 4 hydrogen bonds can be formed between one Arg molecule and one
AP molecule. By taking advantages of this structural selectivity and
high tenability of hydrogen bonds, Arg, immobilized on a graphene
surface via electrostatic interactions, was utilized to structurally
capture AP. Interestingly, bonded AP still remained the perfect electrochemical
activities. The extent of Arg–AP bonds was quantified using
a newly designed electrochemical (EC) sensor. To verify the feasibility
of this novel assay, based on multihydrogen bond manipulated single-molecule
recognition (eMuHSiR), both pharmaceutical and serum sample were examined.
In commercial tablet measurement, no significant difference was seen
between the results of eMuHSiR and other standard methods. For measuring
AP concentration in the mice blood, the substances in serum, such
as sugars and fats, would not bring any interference to the eMuHSiR
in a wide concentration range. This eMuHSiR method opens the way for
future development of small molecule detection for the POC testing