Neurospora TS lethal genes involved in protein production, transport, or quality control.

<p>Neurospora TS lethal genes involved in protein production, transport, or quality control.</p

Temperature shift of growing tips of wild-type, <i>un-7</i> and <i>png-1</i> strains to 37°C.

<p>Panels A–C were taken after shifting overnight slide cultures incubated at room temperature to 37°C for 4 hours and panels D–F show the cultures grown on slides at room temperature. (A,D) Wild type strain 2489. (B, E) <i>png-1</i> strain 9860. (C, F) <i>un-7</i> strain 2176.</p

Selected cosmids spanning the genomic region including NCU00651.

<p>Open reading frames are indicated above the line representing the genome sequence while the cosmids are indicated below the line.</p

Alignment of the putative amino acid sequence from the mutated region of the NCU00651 protein from wild type and two mutants as well as select fungi.

<p>The altered amino acid residues in the UN-7 and PNG-1 proteins are indicated by grey shading and amino acid residue 273 is indicated with an “*” above the sequence. Amino acid residue 279 is indicated with a “∧” above the sequence. The locus designations for other species are as follows: <i>Sordaria macrospora</i> CBI51252; <i>Aspergillus fumigatus</i> EDP54057; <i>Coccidioides immitis</i> CIMG_08062; <i>Magnaporthe grisea</i> MGG_03598; <i>Saccharomyces cerevisiae</i> EE09111; <i>Phycomyces blakesleeanus</i> 178891.</p

Cumulative growth of wild type and mutant strains at 37°.

<p>Values are the average of three measurements plus or minus the standard deviation.</p