Additional file 1 of Immunoregulatory and neutrophil-like monocyte subsets with distinct single-cell transcriptomic signatures emerge following brain injury

Additional file 1: Figure S1. Dil-Liposome localizes in white blood cells, and is distributed in Iba1 + cells of liver and spleen, but not the brain. (A-B) Representative confocal images for brain cortex showing Dil-LPM (red) inside blood vessels (CD31 + , white) and not in microglia (Iba-1 + , green) or brain parenchyma. (E–G) Dil-LPM is not detected in the brain of naïve mice at 1 h (F) or 24 h after injection (G). (H) Representative confocal images showing Dil-LPM (red) staining in Iba1 + (green) cells in kidney, (I) liver parenchyma (J) and spleen. n = 5/group, *P < 0.05; **P < 0.01; ****P < 0.0001. One-way ANOVA with Bonferroni post hoc