A physiological measure of shifting connections in the Rana pipiens retinotectal system

The retinotectal connections of developing Rana tadpoles and froglets have been studied using light-pipe techniques to directly assay the pattern of the projection from the retina to the tectum. The projection site of the retina surrounding the optic nerve head was determined at two different stages of development (late larval and metamorphic frog) on the same animal. Small electrolytic marker lesions were used to mark the tectal sites to which the optic nerve head projected at these two times. Comparison of the positions of the two lesions gives a direct measure of the shift in the projection during the interlesion time interval of one week. The results indicate a shift in the projection of 275 µm week–1 in late larval life. Previous work in Xenopus using the light-pipe techniques indicated a qualitatively similar shift during equivalent stages of development, but significantly smaller in magnitude. In the present study, topographic postsynaptic units could be recorded at all stages investigated, indicating functional synapses between the optic nerve fibres and the tectum. Thus, these studies offer evidence of a significant shift in the functional connection pattern of the amphibian retinotectal map during development, in agreement with the recent anatomical data from other laboratories on the Rana and goldfish visual system

Evaluation of Lumicyano cyanoacrylate fuming process for the development of latent fingermarks on plastic carrier bags by means of a pseudo operational comparative trial

There are a number of studies discussing recent developments of a one-step fluorescent cyanoacrylate process. This study is a pseudo operational trial to compare an example of a one-step fluorescent cyanoacrylate product, Lumicyano™, with the two recommended techniques for plastic carrier bags; cyanoacrylate fuming followed by basic yellow 40 (BY40) dyeing and powder suspensions. 100 plastic carrier bags were collected from the place of work and the items were treated as found without any additional fingermark deposition. The bags were split into three and after treatment with the three techniques a comparable number of fingermarks were detected by each technique (average of 300 fingermarks). The items treated with Lumicyano™ were sequentially processed with BY40 and an additional 43 new fingermarks were detected. Lumicyano™ appears to be a suitable technique for the development of fingermarks on plastic carrier bags and it can help save lab space and time as it does not require dyeing or drying procedures. Furthermore, contrary to other one-step cyanoacrylate products, existing cyanoacrylate cabinets do not require any modification for the treatment of articles with Lumicyano™. To date, there is little peer reviewed articles in the literature on trials related to Lumicyano™ and this study aims to contribute to fill this gap

Pseudo-operational trials of Lumicyano solution and Lumicyano powder for the detection of latent fingermarks on various substrates

This study presents pseudo-operational trials comparing a one-step fluorescent cyanoacrylate process with a number of other enhancement techniques on a variety of substrates. This one-step process involves a product, 4% Lumicyano, which is a solution consisting of 4% by weight of a powdered dye (Lumicyano powder) dissolved in a cyanoacrylate-based solution (Lumicyano solution). The cyanoacrylate in the Lumicyano solution may be of a higher quality than that used in the two-step products.One hundred items were collected from the place of work for each trial. Trial 1 involved a comparison of 4% Lumicyano with the conventional two-step cyanoacrylate fuming-dye staining for the detection of latent fingermarks on plastic carrier bags. Trial 2 assessed the quality of the Lumicyano solution (with no powdered dye) but used in a two-step process with basic yellow 40 (BY40). Trial 1, using 4% Lumicyano powder and traditional cyanoacrylate → BY40 detected a similar amount of fingermarks (~295); however, sequential BY40 treatment (i.e., after 4% Lumicyano) detected an additional 30% marks. Trial 2 resulted in the detection of 565 marks after Lumicyano solution → BY40 in comparison to 489 marks after traditional cyanoacrylate fuming and BY40 staining. Trials 3 through 5 compared 4% Lumicyano, 1,2-indanedione-zinc, and ninhydrin on junk mail, magazines, and cardboard used for food or cosmetic packaging; the detection rate was low for all techniques and substrates. Trial 6 on cardboard packaging using 4% Lumicyano, black iron-oxide powder suspension, and magnetic powder also provided a low detection rate. Trial 7, using 4% Lumicyano → BY40, solvent black 3, and iron-oxide powder suspensions on cardboard packaging from a fast food chain, indicated that 4% Lumicyano → BY40 might be a suitable alternative to solvent black 3 and iron-oxide powder suspensions for suspected greasy marks

The Rarity of Star Formation in Brightest Cluster Galaxies as Measured by WISE

We present the mid-infrared (IR) star formation rates of 245 X-ray selected, nearby (z<0.1) brightest cluster galaxies (BCGs). A homogeneous and volume limited sample of BCGs was created by X-ray selecting clusters with L_x > 1x10^44 erg/s. The Wide-Field Infrared Survey Explorer (WISE) AllWISE Data Release provides the first measurement of the 12 micron star formation indicator for all BCGs in the nearby Universe. Perseus A and Cygnus A are the only galaxies in our sample to have star formation rates of > 40 M_sol/yr, indicating that these two galaxies are highly unusual at current times. Stellar populations of 99 +/- 0.6 % of local BCGs are (approximately) passively evolving, with star formation rates of <10 M_sol/yr. We find that in general, star formation produces only modest BCG growth at the current epoch.Comment: 5 pages, 3 figures, accepted for publication in MNRA

Physico-chemical properties of ionic-liquid water mixtures

In order for Ionic Liquids (ILs) to be utilized to their full potential, it is necessary to have a complete understanding of their physical properties, including phase transitions temperatures1. We have previously reported into the extent of structuring of ILs using photochromic molecular probes, and investigated the appropriate IL water content to yield hydrated IL systems for analysis of polarity and to create environments suitable for effective enzyme activity 1,2,3. In this study we investigated interactions in hydrated ILs containing variable hydrophobic and hydrophilic regions through optical and thermal analysis. The enthalpies and phase transitions of the systems were compared, between the temperature range -50°C to +30°C for the ILs with varying degrees of hydration. Reichardtʼs dye was used as a molecular probe to monitor changes in interactions in the ILs as a function of temperature. Comparisons were made between ILs and for ILs with varying degrees of hydration. Spectroscopic studies were performed using Perkin Elmer UV-Visible Spectrometer and phase transitions monitored using a Perkin Elmer Differential Scanning Calorimeter. The ILs examined are; Trihexyltetradecylphosphonium Chloride [P6,6,6,14 Cl] Tributyl-tetradecylphosphonium Chloride, [P4,4,4,14 Cl], 1-Ethyl-methyl-3-imidazolium-ethyl Sulfate [Emim][EtSO4] and Trihexyltetradecylphosphonium Dicyanamide [P6,6,6,14 DCA]. 1. Robert Byrne, Simon Coleman, Simon Gallagher, and Dermot Diamond. Designer Molecular Probes for Phosphonium Ionic Liquids. Physical Chemistry Chemical Physics, 2010. 2. Kyoko Fujita, Douglas R. MacFarlane, Maria Forsyth, Masahiro Yoshizawa-Fujita, Kenichi Murata,† Nobuhumi Nakamura, and Hiroyuki Ohno*,Solubility and Stability of Cytochrome c in Hydrated Ionic Liquids: Effect of Oxo Acid Residues and Kosmotropicity, 2007. 3. SergeiV.DzyubaandRichardA.Bartsch,Expandingthepolarityrange of ionic liquids, Tetrehedron Letters, 2002

Photo- and solvatochromic properties of nitrobenzospiropyran in ionic liquids containing the [NTf2]- anion

The photo-, thermo- and solvatochromic properties of 2,3-dihydro-10,30,30-trimethyl-6-nitrospiro- [1-benzopyran-2,20-1H-indole] (BSP-NO2) were studied in ILs containing the anion [NTf2]- by UV-Vis absorption spectroscopy, ab initio molecular orbital theory and density functional theory (DFT) calculations. It was found that the kinetics and thermodynamics of the BSP-NO2 MC (merocyanine) equilibrium was sensitive to the nature of the cation. It was also observed that the imidazolium cation can form a through-space orbital interaction with the MC isomer, rather than a simple electrostatic interaction, thus preventing the MC conversion back to the BSP-NO2 isomer. The BSP-NO2 MC equilibrium thus serves as a model system for studying modes of interaction of the cations in ionic liquids

Image patch analysis and clustering of sunspots: a dimensionality reduction approach

Sunspots, as seen in white light or continuum images, are associated with regions of high magnetic activity on the Sun, visible on magnetogram images. Their complexity is correlated with explosive solar activity and so classifying these active regions is useful for predicting future solar activity. Current classification of sunspot groups is visually based and suffers from bias. Supervised learning methods can reduce human bias but fail to optimally capitalize on the information present in sunspot images. This paper uses two image modalities (continuum and magnetogram) to characterize the spatial and modal interactions of sunspot and magnetic active region images and presents a new approach to cluster the images. Specifically, in the framework of image patch analysis, we estimate the number of intrinsic parameters required to describe the spatial and modal dependencies, the correlation between the two modalities and the corresponding spatial patterns, and examine the phenomena at different scales within the images. To do this, we use linear and nonlinear intrinsic dimension estimators, canonical correlation analysis, and multiresolution analysis of intrinsic dimension.Comment: 5 pages, 7 figures, accepted to ICIP 201

Portable Lab-on-a-Disc system integrating photo-switchable micro-valves for in-situ aquatic environmental monitoring

This work describes the first use of a portable centrifugal microfluidic analysis system (CMAS) for on-site lab-on-a-disc water quality monitoring. The centrifugal microfluidic platform designed for the detection of nitrite in multiple water samples incorporates photo-switchable microvalves, which are easily controlled using white light irradiation. Calibration of the CMAS system resulted in a linear response that obeys the Beer-Lambert Law. Excellent correlation of results between the CMAS device and a standard UV-Vis spectrophotometer were obtained