3 research outputs found
Hospitalization due to norovirus and genotypes of rotavirus in pediatric patients, state of EspÃrito Santo
Viruses are the leading cause for hospitalization due to
gastroenteritis worldwide. Group A rotaviruses (RV) are the most
prevalent and are assorted in glycoproteins (G) and protease sensitive
(P) dual genotypes based on polymorphic genes that encode the external
VP7 and VP4 capsid proteins, respectively. Noroviruses (NoV) have
increasingly answered by sporadic gastroenteritis. This study aimed to
determine the prevalence of NoV and RV in 68 hospitalized children,
between July 2004 and November 2006, at a pediatric hospital in
Vitória city, state of EspÃrito Santo, Southeastern Brazil.
Nucleic acid was extracted from fecal suspension following the
guanidine-silica procedure. Reverse transcriptase-polymerase chain
reaction (RT-PCR) and polyacrylamide gel electrophoresis were employed
for NoV and RV detection, respectively. RV genotyping was accomplished
using RT-PCR followed by heminested multiplex PCR with specific primers
for the most prevalent types of G and P. Fecal samples were positive
for NoV and RV in 39.7% (27/68) and 20.5% (14/68), respectively and
together were responsible for 60% (41/68) of the cases. RV genotypes
were: 50% G9P[8], 28.7% G2P[4], 7.1% G1P[8], G2P[8] and G?P[8]. Vomit
was a prominent manifestation observed in 92% and 85% of the NoV and RV
cases, respectively. The median hospitalization was 5 and 5.5 days for
the patients infected with NoV and RV, respectively. The data showed
that NoV prevailed over RV and it also corroborated the emergence of RV
G9 genotype followed by G2P[4], reinforcing the need for RV genotype
surveillance