The Movement Model of Pilots' Visual Attention

This paper describes the application of an important tool which can capture shifting information from pilots' visual attention data. In order to investigate the shifting information, the shifting state space is defined by visual tracking, visual interference and visual dormancy. Using this analysis, the movement of pilots' visual attention can be completely measured. The results that the forecast of the probability shifting model is coincident with the fact suggest the use of the model as a powerful technique for measuring the movement of pilots' visual attention. Furthermore, the link between visual attention and driving experience or sexual distinction are also discussed in the probability shifting model

A Blue Native-PAGE analysis of membrane protein complexes in Clostridium thermocellum

Background Clostridium thermocellum is a Gram-positive thermophilic anaerobic bacterium with the unusual capacity to convert cellulosic biomass into ethanol and hydrogen. Identification and characterization of protein complexes in C. thermocellum are important toward understanding its metabolism and physiology. Results A two dimensional blue native/SDS-PAGE procedure was developed to separate membrane protein complexes of C. thermocellum. Proteins spots were identified by MALDI-TOF/TOF Mass spectrometry. 24 proteins were identified representing 13 distinct protein complexes, including several putative intact complexes. Interestingly, subunits of both the F1-F0-ATP synthase and the V1-V0-ATP synthase were detected in the membrane sample, indicating C. thermocellum may use alternative mechanisms for ATP generation. Conclusion Two dimensional blue native/SDS-PAGE was used to detect membrane protein complexes in C. thermocellum. More than a dozen putative protein complexes were identified, revealing the simultaneous expression of two sets of ATP synthase. The protocol developed in this work paves the way for further functional characterization of these protein complexes

Bacterial community assembly driven by temporal succession rather than spatial heterogeneity in Lake Bosten: a large lake suffering from eutrophication and salinization

Oligosaline lakes in arid and semi-arid regions play a crucial role in providing essential water resources for local populations. However, limited research exists on the impact of the environment on bacterial community structure in these lakes, co-occurrence patterns and the mechanisms governing bacterial community assembly. This study aims to address this knowledge gap by examining samples collected from five areas of Lake Bosten over four seasons. Using the 16S rRNA gene sequencing method, we identified a total of 510 to 1,005 operational taxonomic units (OTUs) belonging to 37 phyla and 359 genera in Lake Bosten. The major bacterial phyla were Proteobacteria (46.5%), Actinobacteria (25.9%), Bacteroidetes (13.2%), and Cyanobacteria (5.7%), while the major genera were hgcI_clade (12.9%), Limnohabitans (6.2%), and Polynucleobacter (4.7%). Water temperature emerged as the primary driver of these community structure variations on global level. However, when considering only seasonal variations, pH and nitrate were identified as key factors influencing bacterial community structures. Summer differed from other seasons in aspects of seasonal symbiotic patterns of bacterial communities, community assembly and function are different from other seasons. There were notable variations in bacterial community structures between winter and summer. Deterministic processes dominated community assembly, but there was an increase in the proportion of stochastic processes during summer. In summer, the functions related to photosynthesis, nitrogen fixation, and decomposition of organic matter showed higher abundance. Our findings shed light on the response of bacterial communities to environmental changes and the underlying mechanisms of community assembly in oligosaline lakes in arid regions

The Rice HGW Gene Encodes a Ubiquitin-Associated (UBA) Domain Protein That Regulates Heading Date and Grain Weight

Heading date and grain weight are two determining agronomic traits of crop yield. To date, molecular factors controlling both heading date and grain weight have not been identified. Here we report the isolation of a hemizygous mutation, heading and grain weight (hgw), which delays heading and reduces grain weight in rice. Analysis of hgw mutant phenotypes indicate that the hemizygous hgw mutation decreases latitudinal cell number in the lemma and palea, both composing the spikelet hull that is known to determine the size and shape of brown grain. Molecular cloning and characterization of the HGW gene showed that it encodes a novel plant-specific ubiquitin-associated (UBA) domain protein localized in the cytoplasm and nucleus, and functions as a key upstream regulator to promote expressions of heading date- and grain weight-related genes. Moreover, co-expression analysis in rice and Arabidopsis indicated that HGW and its Arabidopsis homolog are co-expressed with genes encoding various components of ubiquitination machinery, implying a fundamental role for the ubiquitination pathway in heading date and grain weight control

The functional differentiation of the post-PKS tailoring oxygenases contributed to the chemical diversities of atypical angucyclines

Angucyclines are one of the largest families of aromatic polyketides with various chemical structures and bioactivities. Decades of studies have made it easy for us to depict the picture of their early biosynthetic pathways. Two families of oxygenases, the FAD-dependent oxygenases and the ring opening oxygenases, contribute to the formation of some unique skeletons of atypical angucyclines. The FAD-dependent oxygenases involved in the biosynthetic gene clusters of typical angucyclines catalyze two hydroxylation reactions at C-12 and C-12b of prejadomycin, while their homolog JadH in jadomycin gene cluster catalyze the C-12 hydroxylation and 4a,12b-dehydration reactions of prejadomycin, which leads to the production of dehydrorabelomycin, a common intermediate during the biosynthesis of atypical angucyclines. Ring opening oxygenases of a unique family of oxygenases catalyze the oxidative CC bond cleavage reaction of dehydrorabelomycin, followed by different rearrangement reactions, resulting in the formation of the various chemical skeletons of atypical angucyclines. These results suggested that the functional differentiation of these oxygenases could apparently enrich the sources of aromatic polyketides with greater structure diversities. Keywords: Angucycline, Biosynthesis, Oxygenase, FAD-dependent monooxygenase, Ring opening oxygenas

IIHT: medical report generation with image-to-indicator hierarchical transformer

Automated medical report generation has become increasingly important in medical analysis. It can produce computer-aided diagnosis descriptions and thus significantly alleviate the doctors’ work. Inspired by the huge success of neural machine translation and image captioning, various deep learning methods have been proposed for medical report generation. However, due to the inherent properties of medical data, including data imbalance and the length and correlation between report sequences, the generated reports by existing methods may exhibit linguistic fluency but lack adequate clinical accuracy. In this work, we propose an image-to-indicator hierarchical transformer (IIHT) framework for medical report generation. It consists of three modules, i.e., a classifier module, an indicator expansion module and a generator module. The classifier module first extracts image features from the input medical images and produces disease-related indicators with their corresponding states. The disease-related indicators are subsequently utilised as input for the indicator expansion module, incorporating the “data-text-data” strategy. The transformer-based generator then leverages these extracted features along with image features as auxiliary information to generate final reports. Furthermore, the proposed IIHT method is feasible for radiologists to modify disease indicators in real-world scenarios and integrate the operations into the indicator expansion module for fluent and accurate medical report generation. Extensive experiments and comparisons with state-of-the-art methods under various evaluation metrics demonstrate the great performance of the proposed method.</p

Autonomous Planning and Robust Control for Wheeled Mobile Robot With Slippage Disturbances Based on Differential Flat

Abstract This paper proposes a wheeled mobile robot (WMR) robust control scheme. The feasible strategy is developed to achieve an efficient and robust autonomous mobile robot motion. To realize kinematic autonomous planning and control of the WMR, a novel controller is designed based on control Lyapunov function. This part can be divided into the following two aspects: 1) considering the nonholonomic constraints in the autonomous mobile robot trajectory tracking, a dynamic feedback‐linearization is adopted by utilizing differential flatness‐based integrated control framework to achieve full‐state controllability; 2) to compensate the structured uncertainties and slippage disturbances related to the robot kinematic model, a robust controller is designed based on control Lyapunov function with quadratic programming. Such a strategy can achieve autonomous motion even with unknown slippage disturbances subject to various constraints. Moreover, the sufficient condition is also analyzed to ensure the WMR system exponential stability. The effectiveness and performance of the proposed method are verified by numerical simulation