DNA recovery from microhymenoptera using six non-destructive methodologies with considerations for subsequent preparation of museum slides

Abstract: Due to the tiny size of microhymenoptera, successful morphological identification typically requires specific mounting protocols that require time, skills and experience. Molecular taxonomic identification is an alternative, but many DNA extraction protocols call for maceration of the whole specimen, which is not compatible with preserving museum vouchers. Thus, non-destructive DNA isolation methods are attractive alternatives for obtaining DNA without damaging sample individuals. However, their performance needs to be assessed in microhymenopterans. We evaluated six non-destructive methods: A) DNeasy速 Blood Tissue, B) DNeasy速 modified, C) Protocol with CaCl2 buffer, D) CaCl2 modified, E) HotSHOT modified and F) Direct PCR kit. The performance of each DNA extraction method was tested across several microhymenopteran species by attempting to amplify the mitochondrial gene COI from insect specimens of varying ages: 1 day, 4 months, 3 years, 12 years and 23 years. Methods B and D allowed COI amplification in all insects, while techniques A, C and E were successful in DNA amplification from insects up to 12 years old. The technique F, the fastest, was useful in insects up to 4 months-old. Finally, we adapted permanent slide preparation in Canada balsam for every technique. The results reported allow for combining morphological and molecular methodologies for taxonomic studies.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author

First record of Tamarixia dahlsteni Zuparko (Hymenoptera, Eulophidae), a parasitoid of Trioza eugeniae Froggatt (Hemiptera, Triozidae) and current status of Tamarixia species in Mexico

Parasitic wasps of the genus Tamarixia represent important biological control agents of members of the Psylloidea group and are host specific; therefore, they can be delivery used to control insect pests. In this study we report for the first time the parasitoid Tamarixia dahlsteni in Mexico and its mitochondrial barcode region of the cytochrome oxidase 1 gene (CO1). We also reviewed the species diversity of the genus Tamarixia in Mexico

First record of Tamarixia dahlsteni Zuparko (Hymenoptera, Eulophidae), a parasitoid of Trioza eugeniae Froggatt (Hemiptera, Triozidae) and current status of the Tamarixia species in Mexico

Parasitic wasps of the genus Tamarixia represent important biological control agents of members of the true bug group, Psylloidea, and are host specific; therefore, they can be used to control insect pests. In this study we report, for the first time, the presence of the parasitoid Tamarixia dahlsteni in Mexico and its mitochondrial barcode region of the cytochrome oxidase I gene (COI). We also review the species diversity of the genus Tamarixia in Mexico