Responses to survey questions 10, 15, 16, and 17.

<p>Note: Data may not add up to 100% due to rounding.</p><p>Responses to survey questions 10, 15, 16, and 17.</p

Demographic characteristics of participants surveyed and their association with the likelihood of answering that it is important to ask patients about sexual orientation and gender identity when they register at the health center.

<p>* Statistically significant at the level of p<0.05</p><p>**Category was not included in the chi-square analysis due to small sample size</p><p>Q7. As part of a written registration form, do you think it is important to ask patients about sexual orientation when they register at the health center?</p><p>Q8. As part of a written registration form, do you think it is important to ask patients about gender identity when they register at the health center?</p><p>Note: Data may not add up to 100% due to rounding.</p><p>Demographic characteristics of participants surveyed and their association with the likelihood of answering that it is important to ask patients about sexual orientation and gender identity when they register at the health center.</p

Additional file 2: of Pretreatment loss to follow-up of tuberculosis patients in Chennai, India: a cohort study with implications for health systems strengthening

STROBE checklist for cohort studies. (DOC 85 kb

Additional file 1: of Pretreatment loss to follow-up of tuberculosis patients in Chennai, India: a cohort study with implications for health systems strengthening

Table S1 Revised National Tuberculosis Control Programme (RNTCP) designated microscopy centers (DMCs) in Chennai included in this study. (DOCX 127 kb

Anti-HIV-1 activity and levels of antimicrobials in CVL from HIV(−) women.

<p>Anti-HIV-1 activity and levels of antimicrobials in CVL from HIV(−) women.</p

Measurement of antiviral activity in CVL from HIV(+) women.

<p>Thirty two HIV(+) CVL were diluted 1∶4 and incubated with HIV-1 IIIB (X4-tropic) and BaL (R5-tropic) (Panel A) or NL4.3 (X4) and YU-2.c (R5) (Panel B) at a multiplicity of infection (MOI) of 1 for 1h at 37°C prior to infecting TZM-bl cells. Data points in each graph represents one individual patient. Media, “Control”, and “CVL only” wells (Panel A) were set up as negative controls, and replica wells of virus (IIIB, BaL, NL4.3 and YU-2.c) were set up as positive controls. Significant differences (p values) between the mean of the control (virus alone) and that of the cognate virus+CVL are shown in the Figure.</p

Correlation of levels of antimicrobials and anti-HIV IgG in CVL with anti-HIV activity against 5 different viruses.

<p>Correlation of levels of antimicrobials and anti-HIV IgG in CVL with anti-HIV activity against 5 different viruses.</p

Determination of the presence of infectious HIV-1 in CVL from HIV(+) women.

<p>HIV(+) CVL from 32 patients were diluted 1∶4 and added directly to TZM-bl cells. The assay was terminated 48h post-infection and HIV-1 infection was quantified by measuring luciferase reporter gene activity using a luminometer and expressed as relative light units (RLU). Each data point in graph represents one individual patient. In the Control (media only) column, each point represents replicate wells. When comparing Controls with the CVL samples (TZM-bl cells incubated with CVL), 3 out of 32 showed RLU levels at least 2-fold above background indicating that these 3 CVL contained infectious HIV-1 which trans-activated the LTR-driven reporter gene.</p

HIV-1 Patient Demographics^a.

a<p>CVL were recovered from HIV(+) women and stored in the HERS repository. The lower level of detection of our viral load assay was 400 copies/ml. Women self- identified by race as Black (B), Hispanic (H) or White (W). Also indicated is the age of each individual, plasma viral RNA load (PVL), genital tract viral RNA load (GTVL) and CD4 counts (cells/mm³).</p><p>*Denotes women with STDs; #1, 22, 25 had BV; #11 had Trichomonas; #9, 30 had BV/Trich; #29 had BV/fungus.</p

HIV GP160-specific IgG in CVL from HIV(+) and HIV(−) women.

<p>The results are in milliOD per minute but correspond directly to the amount of binding antibody. 15 is the usual cut-off by this criteria, only two HIV infected person lacked antibody. No antibodies were detected in HIV(−) CVL.</p