1 research outputs found
Bifunctional Glass Membrane Designed to Interface SDS-PAGE Separations of Proteins with the Detection of Peptides by Mass Spectrometry
We describe the construction and
characterization of a novel membrane
designed to allow proteins separated by gel electrophoresis (SDS-PAGE)
to be detected as peptides by mass spectrometry in an efficient and
comprehensive manner. The key attribute of the membrane is a bifunctional
design that allows for the digestion of protein(s) and retention of
the resulting peptides with minimal lateral diffusion. Silane chemistries
are used to differentially treat the opposing surfaces of a glass
filter paper to enable this unique capability