Patient inclusion, overall adequacy for NGS, and reason inadequate for NGS.

<p>Footnote: NGS: Next generation sequencing. ^a DNA quantity < 10 ng. ^b Less than 20% tumor cellularity due to necrosis, fibrosis and quantity of tissue available for analysis.</p

Example of likelihood of adequacy score 3 (high).

<p><b>(A)</b> PET/CT showing right lung lesion that is large and FDG avid. Also, there is no aerated lung in the planned biopsy path. <b>(B)</b> Arrow points to needle in right lung mass.</p

Example of likelihood of adequacy score 2 (equivocal).

<p><b>(A)</b> Chest CT showing right lung nodule that is small and in location (behind rib) requiring angled approach <b>(B)</b> Arrow points to needle in right lung nodule. Small pneumothorax is noted.</p

Univariate logistic regression model of characteristics associated with adequacy for NGS of biopsy specimens.

<p>Univariate logistic regression model of characteristics associated with adequacy for NGS of biopsy specimens.</p

Patient age and days between biopsy and NGS request with adequacy for NGS of biopsy specimens.

<p>Patient age and days between biopsy and NGS request with adequacy for NGS of biopsy specimens.</p

Problematic lesion imaging characteristics affecting likelihood of adequacy score^a.

<p>Problematic lesion imaging characteristics affecting likelihood of adequacy score<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0189651#t001fn001" target="_blank">^a</a>.</p

Biopsy outcome of likelihood of adequacy scores.

<p>Footnote: NGS: Next Generation Sequencing.</p