転写方程式の分子論的解釈

Considering the molecular mechanism, the equation for transcription, which describes the ratio of anygene-transcripts, is derived. RNA polymerase II moves from transcriptional starting point along a gene underaccelerative force F and resistant forces, both a frictional -rx˙ and -sx proportional to traveling distance x. Thenthe equation of motion of RNA polymerase II may be represented by the following, mx¨ = F - rx˙ - sx. In thisequation x, which means traveling distance of polymerase, may be considered as quantity of transcript, and that of inner standard gene is taken to be equal to unit, so that x can be regarded as the ratio of two gene-transcripts.Arranging the coefficients, the equation for transcription x¨ + 2ax˙ + bx = bD is obtained, where a = r/2m isexpected to be constant for any cases and D = F/bm to be almost same value for similar genes of same kinds ofcell. These predictions are examined for 9 kinds of interleukin

マウス抗体クラススィッチ過程の速度論に基づく解析

The class-switching process of immunoglobulin(Ig) gene-transcribing cells was investigated in the thymus of BALB/c female mice. The ratios of IgM,IgG1,IgG3 and IgE-transcripts were determined with aging by realtime PCR. Logarithmic values of IgG1/1gM and IgG3/IgM increased rapidly from birth to several weeks and then gradually. While that of IgE/IgM increased similarly to about 7.5 weeks and it decreased afterwards. Rate theory was applied to analyze these results with supposing both that transcript-quantity was proportional to the number of transcribing cells and that IgM-transcribing cells were supplied continually from stem cells in vivo. On the first order differential concurrent model, in which IgM cells recombined Ig genes and changed to other Ig class-transcribing with rate constants k, the rate equations were solved theoretically. The calculated values of logIgGl/IgM and logIgG3/IgM were good agreement with experimental ones for periods up to 400 days after birth. But, in the case of logIgE/IgM, which had a maximum, this theory could not agree the maximum valueマウス胸腺での抗体遺伝子の転写量比の経時変化を測定し,胸腺でも抗体のクラススィッチ過程が起きていることを示唆した。また,クラススィッチ過程の細胞分化を一次並行反応とみなすことにより,測定結果を速度論で解析する方法を示した。ただし,IgEの結果に関しては,濃度項が分化反応の駆動力である反応速度論では説明できず,他の因子を考慮した方法論が必要であることが判明した

アレキシサイミア ト コドクカン ソーシャル サポート トノ カンレン

The purpose of this study was to examine the relationship between alexithymia，loneliness， and social support. The participants were 313 undergraduate students (159 males and 154 females). Measures were Japanese version of the twenty-item Toronto Alexithymia Scale (TAS-20)， Japanese version of the revised UCLA loneliness scale， and the Scale of Expectancy for Social Support. The participants were divided into three groups (higher， middle，and lower scores) on the basis of their scores on the TAS-20. Results showed that the higher the level of the alexithymia was，the higher the loneliness was. Moreover，it was revealed that the higher the level of the alexithymia was，the lower the expectancy for social support was. This study suggested that alexithymic individuals feel more loneliness，and have lower expectancy for social support than non-alexithymic individuals. It was discussed that the difficulties of identifying feelings and describing feeling，which are two aspects of alexithymia，may cause higher loneliness and lower expectancy for social support

Investigation of simulated annealing method and its application to optimal design of die mold for orientation of magnetic powder

Factors affecting the convergence characteristics and results obtained by the optimal design method using the finite element method and simulated annealing are investigated systematically, and the optimal parameters for simulated annealing method are obtained. The optimal shape of the die mold for orientation of the magnetic powder (nonlinear magnetostatic problem) is obtained using finite elements and simulated annealing. The experimental verification is also carried out </p