Additional file 1: of Role of BMI1 in epithelial ovarian cancer: investigated via the CRISPR/Cas9 system and RNA sequencing

Figure S1. PARP expression detected by western blotting. (PNG 29 kb

Association of the <i>GADD45A (1506T>C)</i> genotype and <i>GADD45A</i> mRNA expression.

<p>A, Relative levels of GADD45A mRNA expression in ovarian cancer tissues compared to normal ovarian tissues. B, Relative levels of GADD45A mRNA expression in the ovarian cancer tissues of patients with different 1506T>C genotypes. C, Relative levels of GADD45A mRNA expression in the normal control tissues of subjects with different 1506T>C genotypes. One spot represents the mean of three independent measurements obtained from one subject. Distributions of the three genotypes were random between the groups. N represents the number of samples in each respective group. Bars represent the standard deviation. Student’s <i>t</i>-test was used to evaluate the differences in the expression levels of different constructs.</p

Sequencing chromatograms of <i>GADD45A (1506T>C)</i>.

<p>A-C, the reverse complement sequencing chromatogram results of the genotypes TT, TC and CC, respectively. The samples were randomly chosen.</p

<i>GADD45A</i> genotypes and ovarian cancer prognosis.

<p>A, Genotype TT+TC of <i>GADD45A</i> had longer relapse-free survival (P = 0.018). B, Genotype TT+TC of <i>GADD45A</i> had longer overall survival (P = 0.0093).</p

The <i>GADD45A</i> (1506T>C) genotype and allele distribution in ovarian cancer and controls.

<p>† The X² for Hardy-Weinberg equilibrium test results of the ovarian cancer group and the control group are 0.51 and 0.47, respectively (both <i>P</i> > 0.05).</p><p>The <i>GADD45A</i> (1506T>C) genotype and allele distribution in ovarian cancer and controls.</p

Kaplan-Meier survival curves of venous thromboembolism (VTE) in ovarian CCC.

<p>(a). Overall survival analyzed for cases with or without VTE. (b). Progression-free survival analyzed for cases with or without VTE. (c) Overall survival analysis based on timing of VTE. (d) Progression-free survival analysis based on the timing of the VTE.</p

Pulmonary emboli in both pulmonary arteries, as demonstrated by autopsy in a patient who died of pulmonary embolism.

<p>Please refer to the main text for the details of the case. Fig. 1a macroscopic thrombus (arrows); Fig. 1b a 20-cm long thrombus; Fig. 1c microscopic thrombus (arrows), hematoxylin and eosin stain, magnification X100.</p

Features of venous thromboembolism (VTE) in ovarian clear cell carcinoma.

<p>Abbreviations: VTE, venous thromboembolism; DVT, deep vein thrombosis; PE, pulmonary embolism.</p><p>Features of venous thromboembolism (VTE) in ovarian clear cell carcinoma.</p

Clinicopathological features of ovarian CCC patients with or without VTE.

<p>*Chi-square test and parametric student <i>t</i> test.</p><p>Abbreviations: CCC, clear cell carcinoma; SD, standard deviation; VTE, venous thromboembolism; CA 125, cancer antigen 125.</p><p>Clinicopathological features of ovarian CCC patients with or without VTE.</p